Contributions
Abstract: EP627
Type: E-Poster Presentation
Session title: Chronic lymphocytic leukemia and related disorders - Biology & Translational Research
Background
Increasing studies have identified that non-coding RNAs play critical roles in the initiation and progression of tumors via participating in competitive endogenous RNA (ceRNA) networks. However, the roles and functions of the ceRNA network in chronic lymphocytic leukemia (CLL) remain poorly understood.
Aims
The present study aims to explore the transcriptomatic profile and construct the distinct ceRNA network in CLL.
Methods
The expression profile of RNAS of CLL patients, CLL cell lines (MEC-1 and EHEB) and healthy group were obtained by the illumina sequencing. R software was used for functional enrichment analysis. The data in the genome microarray map GSE22762 was used for survival analysis. The lncRNA/circRNA-miRNA-mRNA ceRNA networks were visualized by Cytoscape 3.7.2. Four DEmRNAs and the circRNA hsa_circ_0007675/hsa-miR-185-3p/TCF7L1 axis were verified by Quantitative real-time PCR.
Results
In the present study, 57 differentially expressed- (DE-)mRNAs, 1391 DElncRNAs, 335 DEmiRNAs and 10013 DEcircRNAs were identified comparing CLL patients with healthy donors. Meanwhile, 482 mRNAs, 6085 lncRNAs, 302 miRNAs and 1847 circRNAs were explored differently expressed between CLL cell lines and healthy donors. GO and KEGG analysis results showed that these differentially expressed genes (DEGs) are related to the occurrence and development of tumors.The survival analyses showed that 16 DEGs (INIP, IL3RA, CHD1, NLRP12, IL20RB, B3GALT4, SIT1, ACOT8, AGFGR, PCLAF, C19orf18, SELENOS, OR7A17, PCDH7, HNRNPC, PHGDH) were significantly differentially expressed.
The ceRNA network were constructed by Cytoscape software. In total, 11 mRNA nodes, 19 miRNA nodes, 105 lncRNA nodes were identified as differentially expressed profiles between CLL patients and control. Meanwhile, a total of 256 DEcircRNAs, 19 DEmiRNAs, and 11 DEmRNAs were employed to construct the ceRNA network between CLL patients and control. We verified four DEGs (TCF7L1, TRIM34, SLC30A10, HOXD4) and the circRNA hsa_circ_0007675/hsa-miR-185-3p/TCF7L1 axis. Compared with normal people, the expression of these four genes and hsa_circ_0007675 in patient specimens were significantly increased whereas the expression of hsa-miR-185-3p was downregulated (p<0.05) .
Conclusion
In this study, we identified the expression profile of RNAs in CLL patients and CLL cell lines. Functional enrichment analysis and survival analysis revealed the potential functions of DEGs. The ceRNA network we established can help to further understand the pathogenesis of CLL and provide potential prognostic markers and novel therapeutic targets.
Keyword(s): Chronic lymphocytic leukemia, Prognosis
Abstract: EP627
Type: E-Poster Presentation
Session title: Chronic lymphocytic leukemia and related disorders - Biology & Translational Research
Background
Increasing studies have identified that non-coding RNAs play critical roles in the initiation and progression of tumors via participating in competitive endogenous RNA (ceRNA) networks. However, the roles and functions of the ceRNA network in chronic lymphocytic leukemia (CLL) remain poorly understood.
Aims
The present study aims to explore the transcriptomatic profile and construct the distinct ceRNA network in CLL.
Methods
The expression profile of RNAS of CLL patients, CLL cell lines (MEC-1 and EHEB) and healthy group were obtained by the illumina sequencing. R software was used for functional enrichment analysis. The data in the genome microarray map GSE22762 was used for survival analysis. The lncRNA/circRNA-miRNA-mRNA ceRNA networks were visualized by Cytoscape 3.7.2. Four DEmRNAs and the circRNA hsa_circ_0007675/hsa-miR-185-3p/TCF7L1 axis were verified by Quantitative real-time PCR.
Results
In the present study, 57 differentially expressed- (DE-)mRNAs, 1391 DElncRNAs, 335 DEmiRNAs and 10013 DEcircRNAs were identified comparing CLL patients with healthy donors. Meanwhile, 482 mRNAs, 6085 lncRNAs, 302 miRNAs and 1847 circRNAs were explored differently expressed between CLL cell lines and healthy donors. GO and KEGG analysis results showed that these differentially expressed genes (DEGs) are related to the occurrence and development of tumors.The survival analyses showed that 16 DEGs (INIP, IL3RA, CHD1, NLRP12, IL20RB, B3GALT4, SIT1, ACOT8, AGFGR, PCLAF, C19orf18, SELENOS, OR7A17, PCDH7, HNRNPC, PHGDH) were significantly differentially expressed.
The ceRNA network were constructed by Cytoscape software. In total, 11 mRNA nodes, 19 miRNA nodes, 105 lncRNA nodes were identified as differentially expressed profiles between CLL patients and control. Meanwhile, a total of 256 DEcircRNAs, 19 DEmiRNAs, and 11 DEmRNAs were employed to construct the ceRNA network between CLL patients and control. We verified four DEGs (TCF7L1, TRIM34, SLC30A10, HOXD4) and the circRNA hsa_circ_0007675/hsa-miR-185-3p/TCF7L1 axis. Compared with normal people, the expression of these four genes and hsa_circ_0007675 in patient specimens were significantly increased whereas the expression of hsa-miR-185-3p was downregulated (p<0.05) .
Conclusion
In this study, we identified the expression profile of RNAs in CLL patients and CLL cell lines. Functional enrichment analysis and survival analysis revealed the potential functions of DEGs. The ceRNA network we established can help to further understand the pathogenesis of CLL and provide potential prognostic markers and novel therapeutic targets.
Keyword(s): Chronic lymphocytic leukemia, Prognosis