Contributions
Abstract: EP618
Type: E-Poster Presentation
Session title: Chronic lymphocytic leukemia and related disorders - Biology & Translational Research
Background
Chronic lymphocytic leukemia (CLL) is a malignant disorder of mature B lymphocytes, and the precise pathogenesis is unknown at present.
Aims
This study set out to screen the differential expression profile of long non-coding RNA (lncRNA) by microarray and explore the underlying mechanism, biological function, and clinical significance of lncRNA in CLL cells.
Methods
We performed microarray detection to screen the differential expression profile of lncRNA and related experiments to explore the underlying mechanism, biological function, and clinical significance of the lncRNA of interest.
Results
Compared to the lncRNA expression profiles of the control group, we picked out lncRNA LEF1-AS1 for further exploration. By quantitative real-time polymerase chain reaction, we validated that primary CLL cells harbor higher lncRNA LEF1-AS1 levels than normal B cells. Expression of LEF1 on RNA and protein level elevated in the two cell lines with stable overexpression of LEF1-AS1. In the term of biological functions, proliferation rates increased, and apoptosis rates decreased in the LEF1-AS1 overexpressed cell lines, as well as a trend of shortened G0/G1 phase and prolonged G2/M phase in the cell cycle. In primary CLL cells, mRNA expression of LEF1 decreased after negatively regulating the expression of LEF1-AS1. RNA Binding Protein Immunoprecipitation and RNA pull-down demonstrated that LncRNA LEF1-AS1 and LEF1 protein could combine especially. However, we have not determined the prognostic significance of lncRNA LEF1-AS1 in CLL patients.
Conclusion
The thesis concludes that LEF1-AS1 may be a potential oncogenic lncRNA that regulates the expression of the target gene LEF1 by interacting with protein LEF1, which promotes proliferation and suppresses apoptosis of CLL cells.
Keyword(s): Chronic lymphocytic leukemia, Microarray analysis, Oncogene
Abstract: EP618
Type: E-Poster Presentation
Session title: Chronic lymphocytic leukemia and related disorders - Biology & Translational Research
Background
Chronic lymphocytic leukemia (CLL) is a malignant disorder of mature B lymphocytes, and the precise pathogenesis is unknown at present.
Aims
This study set out to screen the differential expression profile of long non-coding RNA (lncRNA) by microarray and explore the underlying mechanism, biological function, and clinical significance of lncRNA in CLL cells.
Methods
We performed microarray detection to screen the differential expression profile of lncRNA and related experiments to explore the underlying mechanism, biological function, and clinical significance of the lncRNA of interest.
Results
Compared to the lncRNA expression profiles of the control group, we picked out lncRNA LEF1-AS1 for further exploration. By quantitative real-time polymerase chain reaction, we validated that primary CLL cells harbor higher lncRNA LEF1-AS1 levels than normal B cells. Expression of LEF1 on RNA and protein level elevated in the two cell lines with stable overexpression of LEF1-AS1. In the term of biological functions, proliferation rates increased, and apoptosis rates decreased in the LEF1-AS1 overexpressed cell lines, as well as a trend of shortened G0/G1 phase and prolonged G2/M phase in the cell cycle. In primary CLL cells, mRNA expression of LEF1 decreased after negatively regulating the expression of LEF1-AS1. RNA Binding Protein Immunoprecipitation and RNA pull-down demonstrated that LncRNA LEF1-AS1 and LEF1 protein could combine especially. However, we have not determined the prognostic significance of lncRNA LEF1-AS1 in CLL patients.
Conclusion
The thesis concludes that LEF1-AS1 may be a potential oncogenic lncRNA that regulates the expression of the target gene LEF1 by interacting with protein LEF1, which promotes proliferation and suppresses apoptosis of CLL cells.
Keyword(s): Chronic lymphocytic leukemia, Microarray analysis, Oncogene