Contributions
Abstract: EP1349
Type: E-Poster Presentation
Session title: Transfusion medicine
Background
Any technological procedure influences the functional activity of platelets. Therefore, to standardize the quality of platelet components (PLTCs), it is important to assess its phenotypic and aggregation properties. According to the immunophenotypic profile of platelets, one can judge its structural integrity and functional activity.
Aims
To study some phenotypic and functional features of leukocyte-depleted (LD) pathogen-reduced platelets obtained by automatic apheresis.
Methods
Phenotypic and functional characteristics were studied on 64 samples obtained from 32 donors of PLTCs. Platelet samples obtained from wholeblood before apheresis, prepared with 3.8% sodium citrate (n = 32) and obtained by automatic apheresis, pathogen-reduced LD (INTERCEPT (n = 23) and MIRASOL (n= 9)) were studied. The expression of adhesion molecules CD 62P, integrin complex (CD41 /CD61) and platelet activation marker CD36 was assessed using flow cytometry. The studies of the functional aggregation activity of platelets were carried out by theturbidimetric method. Statistical analysis was performed using the STATISTICA v.10software. To assess the quality of the functional aggregation activity of platelets by an opticalturbidimetric method, LD pathogen-reduced PLTCs obtained by automatic apheresiswere diluted to a standard platelet concentration (200-250 thousand / μL) with a poolof platelet-free universal plasma obtained from male donors AB ( IV) blood groupRhesus (D)-negative in a ratio of 1: 3 (PLTCs: AB (IV) Rh (D)-negative (diluent))and incubated for 20-30 min.
Results
The ADP-induced (2.5 μM; 5 μM) platelets aggregation activity before apheresis was 48.1 (36.5; 65.8)% and 66.9 (55.6; 75.7)% respectively, which was significantly higher (p <0.05) than one on the 1st day in LD pathogen-reduced platelets obtained by the method of automatic apheresis. The ADP-induced platelets aggregation activity of that PLTC was 7.5 (1.2; 12.3)% for plain and 10 (7.0; 15.4)% for diluted PLTC in case of 2.5 μM ADP and 15.7 (7.1; 20.1)% for plain and 19.4(14.3; 27.1)% for diluted PLTC in case of 5 μM ADPA statistically significant positive correlation was found in platelet samples from whole blood before apheresis between indicators of the ADP-induced (5 μM) platelet aggregation activity 66.9 (55.6; 75.7)% and the relative number of cells expressing CD62P 13.1 (6.4; 20.7)% (R = 0.82, p = 0). A similar positive correlation was determined in LD pathogen-reduced PLTCs afterapheresis which were stored for one day in a thermal shaker at 22 ± 20 C diluted with plasma. In that case ADP-induced (5 μM) platelet aggregation activity was 19.4(14.3; 27.1))% and the relative number of cells expressing CD62P 13.9 (8.0; 19.1)% (R = 0.36, p = 0.003)
Conclusion
Thus, a positive statistically significant correlation was revealed in ADP-induced (5 μM) platelet aggregation activity and the relative number of cells expressing P-selectin (CD62P) for LD pathogen-reduced PLTCs after apheresis after dilution with plasma and one day storage. The data obtained allow us to consider indicators for assessing the aggregation activity of platelets as one of thecharacteristics of quality control of platelet components.
Keyword(s): Apheresis, Pathogen reduction, Platelet aggregation
Abstract: EP1349
Type: E-Poster Presentation
Session title: Transfusion medicine
Background
Any technological procedure influences the functional activity of platelets. Therefore, to standardize the quality of platelet components (PLTCs), it is important to assess its phenotypic and aggregation properties. According to the immunophenotypic profile of platelets, one can judge its structural integrity and functional activity.
Aims
To study some phenotypic and functional features of leukocyte-depleted (LD) pathogen-reduced platelets obtained by automatic apheresis.
Methods
Phenotypic and functional characteristics were studied on 64 samples obtained from 32 donors of PLTCs. Platelet samples obtained from wholeblood before apheresis, prepared with 3.8% sodium citrate (n = 32) and obtained by automatic apheresis, pathogen-reduced LD (INTERCEPT (n = 23) and MIRASOL (n= 9)) were studied. The expression of adhesion molecules CD 62P, integrin complex (CD41 /CD61) and platelet activation marker CD36 was assessed using flow cytometry. The studies of the functional aggregation activity of platelets were carried out by theturbidimetric method. Statistical analysis was performed using the STATISTICA v.10software. To assess the quality of the functional aggregation activity of platelets by an opticalturbidimetric method, LD pathogen-reduced PLTCs obtained by automatic apheresiswere diluted to a standard platelet concentration (200-250 thousand / μL) with a poolof platelet-free universal plasma obtained from male donors AB ( IV) blood groupRhesus (D)-negative in a ratio of 1: 3 (PLTCs: AB (IV) Rh (D)-negative (diluent))and incubated for 20-30 min.
Results
The ADP-induced (2.5 μM; 5 μM) platelets aggregation activity before apheresis was 48.1 (36.5; 65.8)% and 66.9 (55.6; 75.7)% respectively, which was significantly higher (p <0.05) than one on the 1st day in LD pathogen-reduced platelets obtained by the method of automatic apheresis. The ADP-induced platelets aggregation activity of that PLTC was 7.5 (1.2; 12.3)% for plain and 10 (7.0; 15.4)% for diluted PLTC in case of 2.5 μM ADP and 15.7 (7.1; 20.1)% for plain and 19.4(14.3; 27.1)% for diluted PLTC in case of 5 μM ADPA statistically significant positive correlation was found in platelet samples from whole blood before apheresis between indicators of the ADP-induced (5 μM) platelet aggregation activity 66.9 (55.6; 75.7)% and the relative number of cells expressing CD62P 13.1 (6.4; 20.7)% (R = 0.82, p = 0). A similar positive correlation was determined in LD pathogen-reduced PLTCs afterapheresis which were stored for one day in a thermal shaker at 22 ± 20 C diluted with plasma. In that case ADP-induced (5 μM) platelet aggregation activity was 19.4(14.3; 27.1))% and the relative number of cells expressing CD62P 13.9 (8.0; 19.1)% (R = 0.36, p = 0.003)
Conclusion
Thus, a positive statistically significant correlation was revealed in ADP-induced (5 μM) platelet aggregation activity and the relative number of cells expressing P-selectin (CD62P) for LD pathogen-reduced PLTCs after apheresis after dilution with plasma and one day storage. The data obtained allow us to consider indicators for assessing the aggregation activity of platelets as one of thecharacteristics of quality control of platelet components.
Keyword(s): Apheresis, Pathogen reduction, Platelet aggregation