Contributions
Abstract: EP1334
Type: E-Poster Presentation
Session title: Thrombosis and vascular biology - Biology & Translational Research
Background
Inherited thrombophilia is one of the leading causes of coagulating disorders. Prothrombotic disorders are considered to be risk factors for many obstetric complications of placental origin starting with the implantation process and ending with the formation of the placenta (stillbirth, intrauterine growth retardation, severe preeclampsia, and placental abruption, recurrent pregnancy loss).
Aims
To assess the distribution of genes of thrombophilia and prothrombotic conditions (F2: 20210 G>A, F5: 1691 G>A, F7: 10976 G>A, F13: 103 (163) G>T, FGB: -455 G>A, ITGA2: 807 C>T, ITGB3: 1565 T>C, PAI-1: -675 5G>4G) by women with reproductive failure in anamnesis and by healthy women.
Methods
Genes of thrombophilia and prothrombotic conditions genes were carried out by polymerase chain reaction in real time on the detecting amplifier DT-96 (DNA Technology, Russia).
The crtiterion X2 was used for statistical data processing.
Depending on the diagnosis eastablished in the hospital on the basis of the international classification of diseases (ICD-10), 367 examined women were devided into such groups:
group 1 - female infertility, unspecified (ICD-10: N97.9), n=9;
group 2 - female infertility of other origin (ICD-10: N97.8), n=175;
group 3 - two complete or unspecified abortions, without complications (ICD-10: O03.9), n=19;
group 4 - habitual aborter (ICD-10: N96), n=59;
comparison group - no problems with the onset and gestation of pregnancy, two or more children, n=105.
Results
The data of the performed study showed, that the C allele of the ITGB3: 1565 T>C gene was more common in women with other forms of female infertility (ICD-10: N97.8) (34.9% to 22.9% in the comparison group, p<0.05). Polymorphism of the remaining genes (F2: 20210 G>A, F5: 1691 G>A, F7: 10976 G>A, F13: 103 (163) G>T, FGB: -455 G>A, ITGA2: 807 C>T, PAI-1: -675 5G>4G) was detected with comparable frequency in women with and without pathology of pregnancy.
Conclusion
The presence of the allele C of the ITGB3:1565 T>C gene predosposes to female infertility.
Keyword(s): Gene polymorphism, Pregnancy, Thrombophilia
Abstract: EP1334
Type: E-Poster Presentation
Session title: Thrombosis and vascular biology - Biology & Translational Research
Background
Inherited thrombophilia is one of the leading causes of coagulating disorders. Prothrombotic disorders are considered to be risk factors for many obstetric complications of placental origin starting with the implantation process and ending with the formation of the placenta (stillbirth, intrauterine growth retardation, severe preeclampsia, and placental abruption, recurrent pregnancy loss).
Aims
To assess the distribution of genes of thrombophilia and prothrombotic conditions (F2: 20210 G>A, F5: 1691 G>A, F7: 10976 G>A, F13: 103 (163) G>T, FGB: -455 G>A, ITGA2: 807 C>T, ITGB3: 1565 T>C, PAI-1: -675 5G>4G) by women with reproductive failure in anamnesis and by healthy women.
Methods
Genes of thrombophilia and prothrombotic conditions genes were carried out by polymerase chain reaction in real time on the detecting amplifier DT-96 (DNA Technology, Russia).
The crtiterion X2 was used for statistical data processing.
Depending on the diagnosis eastablished in the hospital on the basis of the international classification of diseases (ICD-10), 367 examined women were devided into such groups:
group 1 - female infertility, unspecified (ICD-10: N97.9), n=9;
group 2 - female infertility of other origin (ICD-10: N97.8), n=175;
group 3 - two complete or unspecified abortions, without complications (ICD-10: O03.9), n=19;
group 4 - habitual aborter (ICD-10: N96), n=59;
comparison group - no problems with the onset and gestation of pregnancy, two or more children, n=105.
Results
The data of the performed study showed, that the C allele of the ITGB3: 1565 T>C gene was more common in women with other forms of female infertility (ICD-10: N97.8) (34.9% to 22.9% in the comparison group, p<0.05). Polymorphism of the remaining genes (F2: 20210 G>A, F5: 1691 G>A, F7: 10976 G>A, F13: 103 (163) G>T, FGB: -455 G>A, ITGA2: 807 C>T, PAI-1: -675 5G>4G) was detected with comparable frequency in women with and without pathology of pregnancy.
Conclusion
The presence of the allele C of the ITGB3:1565 T>C gene predosposes to female infertility.
Keyword(s): Gene polymorphism, Pregnancy, Thrombophilia