Contributions
Abstract: EP1330
Type: E-Poster Presentation
Session title: Thrombosis and vascular biology - Biology & Translational Research
Background
High level of ambient air pollution significantly increases thrombotic risk, stroke, myocardial infarction and mortality rates. Furthermore, fine particulate matter size less than 2.5 and 10 microns (PM2.5 and PM10) were associated with disseminated intravascular coagulation (DIC) in animal models. This study describes the hemostatic changes in adults during the seasonal variations of air pollution.
Aims
This study aims to evaluate the short-term effects of high levels of PM2.5 and PM10 on the coagulation tests in the same healthy individuals across the seasonal variations of ambient air pollution.
Methods
This is a prospective study. The data were collected from 30 platelet apheresis donors, during February and July 2020 at The Blood Bank Center, Maharaj Nakorn Chiang Mai Hospital, Thailand. The blood samples were obtained repeatedly at the time of donation, once every 4 weeks for 4 visits. The complete blood count and various coagulation tests, including PT, aPTT, vWF antigen, ristocetin cofactor assay, P-selectin and platelet function analysis, were done. Data of the ambient air pollution was recorded at the central air-quality monitoring station, one kilometer from the hospital center. Mixed effect model was used to analyze the impact of high PM2.5 and PM10 on the blood cell count and the hemostatic tests.
Results
There were 30 healthy volunteers, 29 (96.7%) were men. Mean age was 38.9±8.2 years old. The median levels of PM2.5 at the first, second, third and the fourth visits were 68.4, 35.25, 14.36 and 6.21 µg/m3, and the PM10 levels during each visit were 98.09, 52.13, 25.92 and 15.96 µg/m3, respectively. The hematocrit value was correlated with the PM2.5 and PM10 levels. The white blood cell and platelet count were not affected. P-selectin was significantly associated with the levels of PM2.5 and PM10. The platelet function showed no correlation with the particulate matter level. The elevated PM2.5 and PM10 levels were associated with the shortening of prothrombin time. This effect was not seen in aPTT. The values of PM2.5 and PM10 markedly correlated with the increased von Willebrand function, whereas the von Willebrand antigen remained unchanged.
Conclusion
The elevated PM2.5 and PM10 levels affect coagulation tests, including the shortening of prothrombin time and increased von Willebrand function. These findings wound play a key role in pathogenesis of ambient air pollution-related thrombosis.
Keyword(s): Blood coagulation, Platelet function, Von Willebrand factor (vWF)
Abstract: EP1330
Type: E-Poster Presentation
Session title: Thrombosis and vascular biology - Biology & Translational Research
Background
High level of ambient air pollution significantly increases thrombotic risk, stroke, myocardial infarction and mortality rates. Furthermore, fine particulate matter size less than 2.5 and 10 microns (PM2.5 and PM10) were associated with disseminated intravascular coagulation (DIC) in animal models. This study describes the hemostatic changes in adults during the seasonal variations of air pollution.
Aims
This study aims to evaluate the short-term effects of high levels of PM2.5 and PM10 on the coagulation tests in the same healthy individuals across the seasonal variations of ambient air pollution.
Methods
This is a prospective study. The data were collected from 30 platelet apheresis donors, during February and July 2020 at The Blood Bank Center, Maharaj Nakorn Chiang Mai Hospital, Thailand. The blood samples were obtained repeatedly at the time of donation, once every 4 weeks for 4 visits. The complete blood count and various coagulation tests, including PT, aPTT, vWF antigen, ristocetin cofactor assay, P-selectin and platelet function analysis, were done. Data of the ambient air pollution was recorded at the central air-quality monitoring station, one kilometer from the hospital center. Mixed effect model was used to analyze the impact of high PM2.5 and PM10 on the blood cell count and the hemostatic tests.
Results
There were 30 healthy volunteers, 29 (96.7%) were men. Mean age was 38.9±8.2 years old. The median levels of PM2.5 at the first, second, third and the fourth visits were 68.4, 35.25, 14.36 and 6.21 µg/m3, and the PM10 levels during each visit were 98.09, 52.13, 25.92 and 15.96 µg/m3, respectively. The hematocrit value was correlated with the PM2.5 and PM10 levels. The white blood cell and platelet count were not affected. P-selectin was significantly associated with the levels of PM2.5 and PM10. The platelet function showed no correlation with the particulate matter level. The elevated PM2.5 and PM10 levels were associated with the shortening of prothrombin time. This effect was not seen in aPTT. The values of PM2.5 and PM10 markedly correlated with the increased von Willebrand function, whereas the von Willebrand antigen remained unchanged.
Conclusion
The elevated PM2.5 and PM10 levels affect coagulation tests, including the shortening of prothrombin time and increased von Willebrand function. These findings wound play a key role in pathogenesis of ambient air pollution-related thrombosis.
Keyword(s): Blood coagulation, Platelet function, Von Willebrand factor (vWF)