Contributions
Abstract: EP1161
Type: E-Poster Presentation
Session title: Platelet disorders
Background
Calcium homeostasis is crucial for platelet function and Store Operated Calcium Entry was found to be the main Ca2+ entry mechanism in non-excitable like in platelets1. SOCE relays in the association of the DTS, STIM1, and the plasma membrane calcium channels, Orais and TRCPs2. Changes in the Ca2+ homeostasis of neonate platelets were early described3, but most of the main components of SOCE were unknown by that time1,2, which has been revisited in the present research work
Aims
The aim of the present work is to investigate the possible alteration in the regulatory proteins involved in the intracellular calcium homeostasis in human platelets from neonates respect adults.
Methods
Upon platelet isolation they were subjected to either protein- and mRNA isolation protocols and alternatively were used in functional experiments as well as for analysis of intracellular calcium homeostasis using fura2.
Results
mRNA analysis and protein evaluation by Western blotting revealed interesting differences between neonate platelets and platelets from adults like the one shown in the Figure representing STIM1 and SARAF (Fig A), which are relevant for SOCE and therefore, those changes may explain the physiological and functional changes in neonate platelets previously described by others (Fig B and C)4.
Conclusion
Here, we describe some of these key proteins involved in Ca2+ entry through SOCE that are modify in neonate platelets.
Keyword(s):
Abstract: EP1161
Type: E-Poster Presentation
Session title: Platelet disorders
Background
Calcium homeostasis is crucial for platelet function and Store Operated Calcium Entry was found to be the main Ca2+ entry mechanism in non-excitable like in platelets1. SOCE relays in the association of the DTS, STIM1, and the plasma membrane calcium channels, Orais and TRCPs2. Changes in the Ca2+ homeostasis of neonate platelets were early described3, but most of the main components of SOCE were unknown by that time1,2, which has been revisited in the present research work
Aims
The aim of the present work is to investigate the possible alteration in the regulatory proteins involved in the intracellular calcium homeostasis in human platelets from neonates respect adults.
Methods
Upon platelet isolation they were subjected to either protein- and mRNA isolation protocols and alternatively were used in functional experiments as well as for analysis of intracellular calcium homeostasis using fura2.
Results
mRNA analysis and protein evaluation by Western blotting revealed interesting differences between neonate platelets and platelets from adults like the one shown in the Figure representing STIM1 and SARAF (Fig A), which are relevant for SOCE and therefore, those changes may explain the physiological and functional changes in neonate platelets previously described by others (Fig B and C)4.
Conclusion
Here, we describe some of these key proteins involved in Ca2+ entry through SOCE that are modify in neonate platelets.
Keyword(s):