Contributions
Abstract: PB1774
Type: Publication Only
Session title: Stem cell transplantation - Clinical
Background
Hepcidin is a small peptide that is produced in the liver that is most likely the major regulator of iron. Based upon the importance of iron, multiple mechanisms exist for the regulation of hepcidin. Iron levels, inflammation, erythropoiesis and the combined effects of several proteins expressed on hepatocyte membranes are involved. Growth differentiation factor 15 (GDF15) is a member of the transforming growth factor-b (TGF-b) .GDF15 expression level is usually low in resting cells but may be substantially increased following response to diverse cellular stress signals, such as hypoxia, inflammation, acute tissue injury and during cancer progression
Aims
The aim was to assess the relationship of serum Growth Differentiating factor 15(GDF15) to hepcidin in post transplant adult Egyptian patients as an assessment for iron overload and their relationship to post transplantation complications
Methods
Serum GDF15 and hepcidin were measured using ELISA in 45 post allogenic(23 patients) and autologous(22 patients) bone marrow transplanted patients 1 year posttransplantation in comparison to 15 healthy controls recruited from Bone Marrow Transplantation Unit, Ain Shams University Hospitals
Results
Serum level of GDF15 and hepcidin level were elevated 1 year post allogenic and autologous transplantation in comparison to control group with a statistically significant difference between patients and control(P<0.001). GDF15 and hepcidin were positively correlated with ferritin level(p<0.001). GDF15 and ferritin were positively correlated with acute GVHD and chronic GVHD(P=0.004 and 0.002 respectively), but hepcidin did not show any significant correlation with acute GVHD((P=0.110). Also, GDF15, hepcidin and ferritin were positively correlated with serum levels of ALT and AST in both autologous and allogenic transplanted patients. But, GDF15, hepcidin and ferritin were not correlated with bacterial or viral infections in both allogenic and autologous group of patients
Conclusion
Both GDF15 and hepcidin are useful biomarkers for iron overload late post allogenic and autologous bone marrow transplantation and both can be used as a predictor for posttransplantation complications
Keyword(s): BMT, Hepcidin, Iron overload
Abstract: PB1774
Type: Publication Only
Session title: Stem cell transplantation - Clinical
Background
Hepcidin is a small peptide that is produced in the liver that is most likely the major regulator of iron. Based upon the importance of iron, multiple mechanisms exist for the regulation of hepcidin. Iron levels, inflammation, erythropoiesis and the combined effects of several proteins expressed on hepatocyte membranes are involved. Growth differentiation factor 15 (GDF15) is a member of the transforming growth factor-b (TGF-b) .GDF15 expression level is usually low in resting cells but may be substantially increased following response to diverse cellular stress signals, such as hypoxia, inflammation, acute tissue injury and during cancer progression
Aims
The aim was to assess the relationship of serum Growth Differentiating factor 15(GDF15) to hepcidin in post transplant adult Egyptian patients as an assessment for iron overload and their relationship to post transplantation complications
Methods
Serum GDF15 and hepcidin were measured using ELISA in 45 post allogenic(23 patients) and autologous(22 patients) bone marrow transplanted patients 1 year posttransplantation in comparison to 15 healthy controls recruited from Bone Marrow Transplantation Unit, Ain Shams University Hospitals
Results
Serum level of GDF15 and hepcidin level were elevated 1 year post allogenic and autologous transplantation in comparison to control group with a statistically significant difference between patients and control(P<0.001). GDF15 and hepcidin were positively correlated with ferritin level(p<0.001). GDF15 and ferritin were positively correlated with acute GVHD and chronic GVHD(P=0.004 and 0.002 respectively), but hepcidin did not show any significant correlation with acute GVHD((P=0.110). Also, GDF15, hepcidin and ferritin were positively correlated with serum levels of ALT and AST in both autologous and allogenic transplanted patients. But, GDF15, hepcidin and ferritin were not correlated with bacterial or viral infections in both allogenic and autologous group of patients
Conclusion
Both GDF15 and hepcidin are useful biomarkers for iron overload late post allogenic and autologous bone marrow transplantation and both can be used as a predictor for posttransplantation complications
Keyword(s): BMT, Hepcidin, Iron overload