Contributions
Abstract: PB1536
Type: Publication Only
Session title: Enzymopathies, membranopathies and other anemias
Background
During the last decade, next-generation osmotic gradient ektacytometry, which analyses the red blood cell (RBC) deformability under an osmotic gradient, has become a very useful tool for the diagnosis of RBC membrane disorders, especially for hereditary spherocytosis (HS). The LHUB-ULB is currently the National Reference Center for RBC membrane disorders and ektacytometer is one of its tools.
Aims
The aim of this study was to improve our diagnosis of HS by using ektacytometry.
Methods
Between 2017 and 2020, a total of 124 consecutive EDTA samples from patients with chronic haemolytic anaemia were obtained from our routine workload. They were analyzed prospectively using the osmoscan module of an osmotic gradient ektacytometer. According to our diagnostic algorithm, 66 cases of HS, three hereditary elliptocytosis, one hereditary stomatocytosis, one congenital dyserythropoietic anemia type II and one Southeast Asian ovalocytosis were diagnosed. Finally, 52 patients were classified as “membranopathies excluded”. Osmoscan parameters were expressed as percentage of change compared to the control sample of the day. Cut-off values for the most robust parameters for the diagnosis of HS were established using receiver operating characteristic (ROC) curve analysis.
Results
The best criteria for the diagnosis of HS were the combination of a decreased area under the curve (AUC) of more than 17.9% and an increased minimal osmolality point (Omin) of more than 18.3% compared to the control. Using these established cut-offs, osmotic gradient ektacytometry reported a sensitivity of 93.9 % and a specificity of 91.4% for the diagnosis of HS.
Conclusion
After more than three years of routine use in our laboratory, by using dedicated cut-offs for several parameters obtained by osmotic gradient ektacytometry, the next-generation ektacytometer confirmed its place as a powerful diagnostic tool for the diagnosis of RBC membrane disorders.
Keyword(s): Diagnosis, Hemolytic anemia, Hereditary spherocytosis
Abstract: PB1536
Type: Publication Only
Session title: Enzymopathies, membranopathies and other anemias
Background
During the last decade, next-generation osmotic gradient ektacytometry, which analyses the red blood cell (RBC) deformability under an osmotic gradient, has become a very useful tool for the diagnosis of RBC membrane disorders, especially for hereditary spherocytosis (HS). The LHUB-ULB is currently the National Reference Center for RBC membrane disorders and ektacytometer is one of its tools.
Aims
The aim of this study was to improve our diagnosis of HS by using ektacytometry.
Methods
Between 2017 and 2020, a total of 124 consecutive EDTA samples from patients with chronic haemolytic anaemia were obtained from our routine workload. They were analyzed prospectively using the osmoscan module of an osmotic gradient ektacytometer. According to our diagnostic algorithm, 66 cases of HS, three hereditary elliptocytosis, one hereditary stomatocytosis, one congenital dyserythropoietic anemia type II and one Southeast Asian ovalocytosis were diagnosed. Finally, 52 patients were classified as “membranopathies excluded”. Osmoscan parameters were expressed as percentage of change compared to the control sample of the day. Cut-off values for the most robust parameters for the diagnosis of HS were established using receiver operating characteristic (ROC) curve analysis.
Results
The best criteria for the diagnosis of HS were the combination of a decreased area under the curve (AUC) of more than 17.9% and an increased minimal osmolality point (Omin) of more than 18.3% compared to the control. Using these established cut-offs, osmotic gradient ektacytometry reported a sensitivity of 93.9 % and a specificity of 91.4% for the diagnosis of HS.
Conclusion
After more than three years of routine use in our laboratory, by using dedicated cut-offs for several parameters obtained by osmotic gradient ektacytometry, the next-generation ektacytometer confirmed its place as a powerful diagnostic tool for the diagnosis of RBC membrane disorders.
Keyword(s): Diagnosis, Hemolytic anemia, Hereditary spherocytosis