Contributions
Abstract: PB2531
Type: Publication Only
Background
HPA (Human Platelet Alloantigens) are polymorphisms expressed on platelet membrane glycoproteins, several studies have shown the role of antibodies against human platelet antigen (HPA)-15 in platelet alloimmunisation that may show clinical manifestations, such as neonatal alloimmune thrombocytopenia, post-transfusion purpura and platelet refractoriness. Typing of HPA-15a and HPA- 15b has not been carried on the Moroccan population. This is the first study of HPA-15 system in Morocco
Aims
The aim of this study is to determine the genotypes and allele frequencies of HPA-15a and HPA-15b among Moroccan blood donors and to compare their allele frequencies with those reported in other populations
Methods
A total of 97 Moroccan blood donor samples randomly selected, were genotyped using the polymerase chain reaction with sequence-specific primers (PCR-SSP)
Results
The results showed that the most frequent genotypes were AA (43, 29%) followed by AB (29, 89%). Allele frequencies were 0,582 and 0.417 for HPA-15a and HPA-15b, respectively. Allele frequencies were similar to those found in North Africa (Algerian). However, the B allele was less frequent in HPA-15 systems when compared with Caucasians (French) and Arabs (Saudi)
Conclusion
Differences in the distribution of HPA-15 highlight the diversity in Moroccan population. Therefore it is advantageous to establish a compatible platelet concentrates, for patients who require multiple platelets transfusions and have a higher chance of becoming alloimmunised or refractory due to anti-HPA alloantibodies. This data obtained will form an addition to the literature in transfusion research
Session topic: 32. Transfusion medicine
Keyword(s): Gene expression, PCR, Platelet, transfusion
Abstract: PB2531
Type: Publication Only
Background
HPA (Human Platelet Alloantigens) are polymorphisms expressed on platelet membrane glycoproteins, several studies have shown the role of antibodies against human platelet antigen (HPA)-15 in platelet alloimmunisation that may show clinical manifestations, such as neonatal alloimmune thrombocytopenia, post-transfusion purpura and platelet refractoriness. Typing of HPA-15a and HPA- 15b has not been carried on the Moroccan population. This is the first study of HPA-15 system in Morocco
Aims
The aim of this study is to determine the genotypes and allele frequencies of HPA-15a and HPA-15b among Moroccan blood donors and to compare their allele frequencies with those reported in other populations
Methods
A total of 97 Moroccan blood donor samples randomly selected, were genotyped using the polymerase chain reaction with sequence-specific primers (PCR-SSP)
Results
The results showed that the most frequent genotypes were AA (43, 29%) followed by AB (29, 89%). Allele frequencies were 0,582 and 0.417 for HPA-15a and HPA-15b, respectively. Allele frequencies were similar to those found in North Africa (Algerian). However, the B allele was less frequent in HPA-15 systems when compared with Caucasians (French) and Arabs (Saudi)
Conclusion
Differences in the distribution of HPA-15 highlight the diversity in Moroccan population. Therefore it is advantageous to establish a compatible platelet concentrates, for patients who require multiple platelets transfusions and have a higher chance of becoming alloimmunised or refractory due to anti-HPA alloantibodies. This data obtained will form an addition to the literature in transfusion research
Session topic: 32. Transfusion medicine
Keyword(s): Gene expression, PCR, Platelet, transfusion