Contributions
Abstract: PB2529
Type: Publication Only
Background
Platelet membrane carries a large number of glycoproteins, which express the platelet’s specific antigens HPA (Human Platelet Antigen) on their surface. In transfusion, anti-HPA alloimmunization can lead to many serious complications, such as multi-platelet transfusion refractoriness. However, the implication of these antigens in platelet alloimmunization was not studied before in Morocco.
Aims
The aim of this study is to determine the prevalence of the anti-HPA antibodies in serum of 90 multi-transfused patients in Morocco.
Methods
The serum of 90 multi-transfused patients who received leukocyte depleted red cells component, were included in this study, the detection of HPA antibodies was performed by a qualitative solid phase enzyme linked immunesorbent assay kit (PAKPLUS).
Results
A total of 6 out of 90 (6.66 %) patients were found to be immunized to platelets. 1.1% patients were found to be alloimmunized to HPA-5a, 1.1% anti-GPIIb IIIa, 2.22% anti-GPIa IIa, and 2.22% anti- GPIa IIa.
Conclusion
Using a leukocyte depleted red cells component could reduce alloimmunization to platelets. To confirm these data, it’s important to study the association between platelet antigens and their clinical consequences for the proper diagnosis and a better blood transfusion safety.
Session topic: 32. Transfusion medicine
Keyword(s): Platelet, transfusion, Immunity
Abstract: PB2529
Type: Publication Only
Background
Platelet membrane carries a large number of glycoproteins, which express the platelet’s specific antigens HPA (Human Platelet Antigen) on their surface. In transfusion, anti-HPA alloimmunization can lead to many serious complications, such as multi-platelet transfusion refractoriness. However, the implication of these antigens in platelet alloimmunization was not studied before in Morocco.
Aims
The aim of this study is to determine the prevalence of the anti-HPA antibodies in serum of 90 multi-transfused patients in Morocco.
Methods
The serum of 90 multi-transfused patients who received leukocyte depleted red cells component, were included in this study, the detection of HPA antibodies was performed by a qualitative solid phase enzyme linked immunesorbent assay kit (PAKPLUS).
Results
A total of 6 out of 90 (6.66 %) patients were found to be immunized to platelets. 1.1% patients were found to be alloimmunized to HPA-5a, 1.1% anti-GPIIb IIIa, 2.22% anti-GPIa IIa, and 2.22% anti- GPIa IIa.
Conclusion
Using a leukocyte depleted red cells component could reduce alloimmunization to platelets. To confirm these data, it’s important to study the association between platelet antigens and their clinical consequences for the proper diagnosis and a better blood transfusion safety.
Session topic: 32. Transfusion medicine
Keyword(s): Platelet, transfusion, Immunity