Contributions
Abstract: PB1969
Type: Publication Only
Background
A significant number of cancer patients need correction of anemia. Anaemia has a negative impact on quality of life as well as survival. Iron metabolism disorder and cytokines plays important roles in the pathogenesis of this anaemia. The mechanism of their action needs specification.
Aims
To study the aspects of iron transfer and influence of interleukin-6 (IL-6), interleukin-10 (IL-10) and tumor necrosis factor-alpha (TNF-) on progression of anemia in patients with solid tumors
Methods
Sixty-three patients (forty one patients with anaemia/ twenty two patients without anaemia) with Stage II-IV cancer were examined. RBC count, WBC count were performed, levels of HB, HCT, MCV, MCH, MCHC were determined using Sysmex XS-500i analyser (Japan). Serum iron concentration, total iron binding capacity (TIBC), ferritin level, C-reactive protein (CRP) level, transferrin saturation index (TSI) were determined using Olympus Au 480 analyser, (Beckman Coulter, the USA). Concentration of transferrin was determined by Siemens Admia 1200 analyser (Diamond Diagnostics, the USA). Concentrations of IL-6, IL-10 and TNF-α were determined using Stat Fax 2100 analyser (Awareness Technology Inc., the USA). Mann-Whitney U Test was applied to check for statistically significant differences in study samples. In order to evaluate the relation between the variables Spearman correlation coefficient (r) was calculated.
Results
Patients with anaemia show lower concentrations in comparison with patients without anaemia: serum iron (5.5 [IQR, 2.9-7.7] versus 10.9 [IQR, 7.9-14.7]; p<0.05), TSI (11.4 [IQR, 5.1-14.3] versus 17.7 [IQR, 12.5-23.7]; p<0.05), TIBC (50.2 [IQR, 39-60] versus 64.2 [55.5-73.1]; p<0.05), lower levels of HCT (30.1 [IQR, 26.9-33.8] versus 40 [36.5-44.5]; p<0.05), MCH (26.9 [IQR, 24.8-29] versus 29.4 [27.2-31.6]; p<0.05), MCHC (318.9 [IQR, 302.5-331] versus 338.8 [327.5-350]; p<0.05), and higher concentrations of CRP (103.7 [IQR, 32.1-155] versus 34.5 [IQR, 9.3-65.7]; p<0.05), IL-6 (41.5 [IQR, 3.8-31.1] versus 7.1 [IQR, 0.00-9.4]; p<0.05), IL-10 (18.3 [IQR, 4.5-14.4] versus 0.9 [IQR, 0.3-5.5]; p<0.05) and TNF-α (58.6 [IQR, 36.1-81.1] versus 8.25 [IQR, 1.3-13.6]; p<0.05). Concentrations of ferritin, transferrin and MCV level in both groups were the same (p>0.05). As for iron, there was a correlation with HB level (r=0.37) and no correlation with RBC level was found (r<0,3). A correlation with levels of RBC (r=-0.55), HB (r=-0.52), HCT (r=-0.51) and WBC (r=0.45) was found for IL-6. A correlation with level of HB (r=-0.64) was found for IL-10. A correlation with levels of RBC (r=-0.74), HB (r=-0.69), HCT (r=-0.63), WBC (r=0.39) was found for TNF-α. A correlation with WBC (r=0.77), HB (r=-0.74), HCT (r=-0.71), RBC (r=-0.6), MCH (r=-0.32), and MCHC (r=-0.54) levels was found for IL-10.
Conclusion
Data obtained prove the fact that anaemia in cancer patients is associated with progression of functional deficit of iron. A correlation between IL-6, IL-10, TNF-α and hemogram items indicates their significant influence on anaemia progression and evidences its complicated nature, which is not limited by iron deficit only. Mechanism and intensity of cytokines influence on progression of anaemia in cancer patients need further clarification
Session topic: 29. Enzymopathies, membranopathies and other anemias
Keyword(s): Anemia, Cancer, IL-10, IL-6
Abstract: PB1969
Type: Publication Only
Background
A significant number of cancer patients need correction of anemia. Anaemia has a negative impact on quality of life as well as survival. Iron metabolism disorder and cytokines plays important roles in the pathogenesis of this anaemia. The mechanism of their action needs specification.
Aims
To study the aspects of iron transfer and influence of interleukin-6 (IL-6), interleukin-10 (IL-10) and tumor necrosis factor-alpha (TNF-) on progression of anemia in patients with solid tumors
Methods
Sixty-three patients (forty one patients with anaemia/ twenty two patients without anaemia) with Stage II-IV cancer were examined. RBC count, WBC count were performed, levels of HB, HCT, MCV, MCH, MCHC were determined using Sysmex XS-500i analyser (Japan). Serum iron concentration, total iron binding capacity (TIBC), ferritin level, C-reactive protein (CRP) level, transferrin saturation index (TSI) were determined using Olympus Au 480 analyser, (Beckman Coulter, the USA). Concentration of transferrin was determined by Siemens Admia 1200 analyser (Diamond Diagnostics, the USA). Concentrations of IL-6, IL-10 and TNF-α were determined using Stat Fax 2100 analyser (Awareness Technology Inc., the USA). Mann-Whitney U Test was applied to check for statistically significant differences in study samples. In order to evaluate the relation between the variables Spearman correlation coefficient (r) was calculated.
Results
Patients with anaemia show lower concentrations in comparison with patients without anaemia: serum iron (5.5 [IQR, 2.9-7.7] versus 10.9 [IQR, 7.9-14.7]; p<0.05), TSI (11.4 [IQR, 5.1-14.3] versus 17.7 [IQR, 12.5-23.7]; p<0.05), TIBC (50.2 [IQR, 39-60] versus 64.2 [55.5-73.1]; p<0.05), lower levels of HCT (30.1 [IQR, 26.9-33.8] versus 40 [36.5-44.5]; p<0.05), MCH (26.9 [IQR, 24.8-29] versus 29.4 [27.2-31.6]; p<0.05), MCHC (318.9 [IQR, 302.5-331] versus 338.8 [327.5-350]; p<0.05), and higher concentrations of CRP (103.7 [IQR, 32.1-155] versus 34.5 [IQR, 9.3-65.7]; p<0.05), IL-6 (41.5 [IQR, 3.8-31.1] versus 7.1 [IQR, 0.00-9.4]; p<0.05), IL-10 (18.3 [IQR, 4.5-14.4] versus 0.9 [IQR, 0.3-5.5]; p<0.05) and TNF-α (58.6 [IQR, 36.1-81.1] versus 8.25 [IQR, 1.3-13.6]; p<0.05). Concentrations of ferritin, transferrin and MCV level in both groups were the same (p>0.05). As for iron, there was a correlation with HB level (r=0.37) and no correlation with RBC level was found (r<0,3). A correlation with levels of RBC (r=-0.55), HB (r=-0.52), HCT (r=-0.51) and WBC (r=0.45) was found for IL-6. A correlation with level of HB (r=-0.64) was found for IL-10. A correlation with levels of RBC (r=-0.74), HB (r=-0.69), HCT (r=-0.63), WBC (r=0.39) was found for TNF-α. A correlation with WBC (r=0.77), HB (r=-0.74), HCT (r=-0.71), RBC (r=-0.6), MCH (r=-0.32), and MCHC (r=-0.54) levels was found for IL-10.
Conclusion
Data obtained prove the fact that anaemia in cancer patients is associated with progression of functional deficit of iron. A correlation between IL-6, IL-10, TNF-α and hemogram items indicates their significant influence on anaemia progression and evidences its complicated nature, which is not limited by iron deficit only. Mechanism and intensity of cytokines influence on progression of anaemia in cancer patients need further clarification
Session topic: 29. Enzymopathies, membranopathies and other anemias
Keyword(s): Anemia, Cancer, IL-10, IL-6