Contributions
Abstract: PB1971
Type: Publication Only
Background
A significant number of cancer patients need correction of anemia. Iron metabolism disorder and play important roles in the pathogenesis of this anaemia. Hepcidin is the main circulating in the blood regulator of iron absorption and its distribution in tissues. Soluble transferrin receptor binds transferrin and the resulting complex transports iron into the cell. The importance of hepcidin and soluble transferrin receptor in pathogenesis of anemia in patients with solid malignant tumors requires clarification.
Aims
To study the aspects of hepcidin and soluble transferrin receptor (sTfR) release in patients with solid malignant tumor and anaemia, and its relation with values of iron metabolism, interleukin-6 (IL-6), interleukin-10 (IL-10) and tumour necrosis factor-alpha (TNF-)
Methods
Sixty-three patients (41 patients with anaemia/ 22 patients without anaemia) with Stage II-IV cancer were examined. RBC count was performed, levels of HB, HCT, MCV, MCH, MCHC were determined using Sysmex XS-500i analyser (Japan). Serum iron concentration, total iron binding capacity (TIBC), ferritin level, C-reactive protein (CRP) level, transferrin saturation index (TSI) were determined using Olympus Au 480 analyser, (Beckman Coulter, the USA). Concentration of transferrin was determined by Siemens Admia 1200 analyser (Diamond Diagnostics, the USA). Concentrations of IL-6, IL-10 and TNF-α were determined using Stat Fax 2100 analyser (Awareness Technology Inc., the USA). Concentration of sTfR was determined using ACCESS analyser (BeckmanCoulter, the USA). Hepcidin concentration was determined using Charity photometer (Promnauchpribor, Russia). Mann-Whitney U Test was applied to check for statistically significant differences in study samples. In order to evaluate the relation between the variables Spearman correlation coefficient (r) was calculated.
Results
Patients with anaemia show higher concentrations in comparison with patients without anaemia: hepcidin (47.8 [IQR, 50-57.8] versus 33.6 [IQR, 21.1-50]; p<0.05), sTfR (30.7 [IQR, 16.4-63.3] versus 17.3 [IQR, 14.9-19.2]; p<0.05). A correlation with levels of RBC (r=-0.41), HCT (r=-0.35), CRP (r=0.49), TIBC (r=-0.51), ferritin (r=0.61), transferrin (r=-0.55), IL-6 (r=0.52), TNF-α (r=-0.41) was found for hepcidin. A correlation with levels of HB (r=-0.57), HCT (r=-0.5), transferrin (r=0.41), IL-10 (r=0.57) was found for sTfR.
Conclusion
higher concentration of hepcidin and sTfR in patients with solid malignant tumor and anaemia in comparison with patients without anaemia, as long as its correlation with values of RBC, HB and HCT evidence the participation of hepcidin and sTfR in progression of anaemia in these patients. Correlations between hepcidin, sTfR and values of iron metabolism indicate that these proteins effect on progression of anaemia through its influence on iron metabolism. The correlations between hepcidin, IL-6 and IL-10, as well as correlations between sTfR and IL-10 values evidence a regulating role of cytokines in synthesis of reviewed transferrin receptors.
Session topic: 29. Enzymopathies, membranopathies and other anemias
Keyword(s): Anemia, Cancer, hepcidin, Transferrin receptor
Abstract: PB1971
Type: Publication Only
Background
A significant number of cancer patients need correction of anemia. Iron metabolism disorder and play important roles in the pathogenesis of this anaemia. Hepcidin is the main circulating in the blood regulator of iron absorption and its distribution in tissues. Soluble transferrin receptor binds transferrin and the resulting complex transports iron into the cell. The importance of hepcidin and soluble transferrin receptor in pathogenesis of anemia in patients with solid malignant tumors requires clarification.
Aims
To study the aspects of hepcidin and soluble transferrin receptor (sTfR) release in patients with solid malignant tumor and anaemia, and its relation with values of iron metabolism, interleukin-6 (IL-6), interleukin-10 (IL-10) and tumour necrosis factor-alpha (TNF-)
Methods
Sixty-three patients (41 patients with anaemia/ 22 patients without anaemia) with Stage II-IV cancer were examined. RBC count was performed, levels of HB, HCT, MCV, MCH, MCHC were determined using Sysmex XS-500i analyser (Japan). Serum iron concentration, total iron binding capacity (TIBC), ferritin level, C-reactive protein (CRP) level, transferrin saturation index (TSI) were determined using Olympus Au 480 analyser, (Beckman Coulter, the USA). Concentration of transferrin was determined by Siemens Admia 1200 analyser (Diamond Diagnostics, the USA). Concentrations of IL-6, IL-10 and TNF-α were determined using Stat Fax 2100 analyser (Awareness Technology Inc., the USA). Concentration of sTfR was determined using ACCESS analyser (BeckmanCoulter, the USA). Hepcidin concentration was determined using Charity photometer (Promnauchpribor, Russia). Mann-Whitney U Test was applied to check for statistically significant differences in study samples. In order to evaluate the relation between the variables Spearman correlation coefficient (r) was calculated.
Results
Patients with anaemia show higher concentrations in comparison with patients without anaemia: hepcidin (47.8 [IQR, 50-57.8] versus 33.6 [IQR, 21.1-50]; p<0.05), sTfR (30.7 [IQR, 16.4-63.3] versus 17.3 [IQR, 14.9-19.2]; p<0.05). A correlation with levels of RBC (r=-0.41), HCT (r=-0.35), CRP (r=0.49), TIBC (r=-0.51), ferritin (r=0.61), transferrin (r=-0.55), IL-6 (r=0.52), TNF-α (r=-0.41) was found for hepcidin. A correlation with levels of HB (r=-0.57), HCT (r=-0.5), transferrin (r=0.41), IL-10 (r=0.57) was found for sTfR.
Conclusion
higher concentration of hepcidin and sTfR in patients with solid malignant tumor and anaemia in comparison with patients without anaemia, as long as its correlation with values of RBC, HB and HCT evidence the participation of hepcidin and sTfR in progression of anaemia in these patients. Correlations between hepcidin, sTfR and values of iron metabolism indicate that these proteins effect on progression of anaemia through its influence on iron metabolism. The correlations between hepcidin, IL-6 and IL-10, as well as correlations between sTfR and IL-10 values evidence a regulating role of cytokines in synthesis of reviewed transferrin receptors.
Session topic: 29. Enzymopathies, membranopathies and other anemias
Keyword(s): Anemia, Cancer, hepcidin, Transferrin receptor