Contributions
Abstract: PB2507
Type: Publication Only
Background
Microcytic anemia is frequently due to iron deficiency anemia (IDA) or thalassemia. The differentiation between thalassemia and IDA has important clinical implications.
Aims
To study the possibility of using erythrocyte indices as laboratory markers for screening thalassemia.
Methods
The study included 48 patients with a beta-thalassemia minor (26 male and 22 female, 2 to 58 years old) and 50 patients with IDA with Hb>90 g/l (22 male and 28 female, 2 to 54 years ).
The Sysmex XN-2000 analyzer was used to determine: Hb, RBC, MCV, MCH, RDW,% Micro,% Hypo-He. The erythrocyte indices were calculated: E = MCV - (10 × RBC), E&F = MCV - RBC - 5 × Hb - 3.4, G&K = MCV2 × RDW/100 × Hb; M = MCV/RBC; Si = MCV-RBC-3 × Hb; S = MCH/RBC; RDWDI = MCV × RDW/RBC; M-H =%MicroR - %Hypo-He; M-H-RDW =%MicroR - %Hypo-He - RDW-CV.
The concentration of iron and serum ferritin was determined using a Cobas-6000 analyzer. Thalassemia was confirmed by determining hemoglobin fractions (HbA2>3.2%) by capillary electrophoresis (Minicap, Sebia).
Results
The most sensitive were M-H-RDW (97.8%) and M-H (95.4%), G&K (90.1%) and Si (90.1%) , specificity - M (97.5%), Si (97.5) and MH-RDW (96.5%). The best Youden index, which combines information on sensitivity and specificity, were in the indices M-H-RDW (94.3) and M-H (89.9).
Conclusion
The M-H-RDW and M-H indices, which are calculated using the new hematological parameters available on Sysmex analyzers, can be highly reliable for thalassemia screening.
Session topic: 28. Thalassemias
Keyword(s): Iron deficiency anemia, Thalassemia
Abstract: PB2507
Type: Publication Only
Background
Microcytic anemia is frequently due to iron deficiency anemia (IDA) or thalassemia. The differentiation between thalassemia and IDA has important clinical implications.
Aims
To study the possibility of using erythrocyte indices as laboratory markers for screening thalassemia.
Methods
The study included 48 patients with a beta-thalassemia minor (26 male and 22 female, 2 to 58 years old) and 50 patients with IDA with Hb>90 g/l (22 male and 28 female, 2 to 54 years ).
The Sysmex XN-2000 analyzer was used to determine: Hb, RBC, MCV, MCH, RDW,% Micro,% Hypo-He. The erythrocyte indices were calculated: E = MCV - (10 × RBC), E&F = MCV - RBC - 5 × Hb - 3.4, G&K = MCV2 × RDW/100 × Hb; M = MCV/RBC; Si = MCV-RBC-3 × Hb; S = MCH/RBC; RDWDI = MCV × RDW/RBC; M-H =%MicroR - %Hypo-He; M-H-RDW =%MicroR - %Hypo-He - RDW-CV.
The concentration of iron and serum ferritin was determined using a Cobas-6000 analyzer. Thalassemia was confirmed by determining hemoglobin fractions (HbA2>3.2%) by capillary electrophoresis (Minicap, Sebia).
Results
The most sensitive were M-H-RDW (97.8%) and M-H (95.4%), G&K (90.1%) and Si (90.1%) , specificity - M (97.5%), Si (97.5) and MH-RDW (96.5%). The best Youden index, which combines information on sensitivity and specificity, were in the indices M-H-RDW (94.3) and M-H (89.9).
Conclusion
The M-H-RDW and M-H indices, which are calculated using the new hematological parameters available on Sysmex analyzers, can be highly reliable for thalassemia screening.
Session topic: 28. Thalassemias
Keyword(s): Iron deficiency anemia, Thalassemia