Contributions
Abstract: PB1989
Type: Publication Only
Background
The low number of umbilical cord blood cells is an important barrier to successful bone marrow transplantation. Therefore, the growth of these cells while maintaining their functional characteristics is of great importance. Using microfluidic technology as well as culturing cells on 3D scaffolds like DBM has greatly contributed to the simulation of the mechanical and chemical micro-environment of the bone marrow tissue. There are also specific compounds for proliferation without differentiation of cells, one of them is nicotinamide
Aims
In the study we aimed to expand hematopoietic stem cells by nicotinamide and in the 3D scaffold (DBM) under the effect of microfluidic device.
Methods
hematopoietic stem cells(HSCs) were cultured 7 days in two groups: 1. In DBM in the microfloid device 2. In DBM in static state (control) , under the following conditions: 1.negative control 2.cytokine 3.nicotinamide 4.nicotinamide+cytokine 5.negative control with feeder 6.cytokine+feeder 7.nicotinamide+feeder 8.nicotinamide+cytokine+feeder After 7 days, cell count, their purity using flow cytometry, Colony forming unit assay and apoptosis were evaluated. The status of the cells on the scaffold was observed using electron microscopy
Results
Our results indicated that more cells were counted in static culture than microfluidic culture (perfusion), while the ability of cell colonization in microfluidic culture was far higher. The purity of HSCs was higher in microfluidic culture, and fewer cells entered the apoptotic phase. In all cases, the combination of nicotinamide and cytokine in the presence of feeder, was accompanied with more proliferation, more CD34 + cells, less apoptosis, and higher colonyforming ability than other groups.
Conclusion
Nicotinamide by preventing epigenetic changes resulting from laboratorial cell culture and microfluidic device associated with DBM by simulating bone marrow niche is a very suitable compound for the development of these cells.
Session topic: 24. Hematopoiesis, stem cells and microenvironment
Keyword(s): Hematopoietic Stem Cell, Mesenchymal stem cell, Stem cell expansion, Stem cell niche
Abstract: PB1989
Type: Publication Only
Background
The low number of umbilical cord blood cells is an important barrier to successful bone marrow transplantation. Therefore, the growth of these cells while maintaining their functional characteristics is of great importance. Using microfluidic technology as well as culturing cells on 3D scaffolds like DBM has greatly contributed to the simulation of the mechanical and chemical micro-environment of the bone marrow tissue. There are also specific compounds for proliferation without differentiation of cells, one of them is nicotinamide
Aims
In the study we aimed to expand hematopoietic stem cells by nicotinamide and in the 3D scaffold (DBM) under the effect of microfluidic device.
Methods
hematopoietic stem cells(HSCs) were cultured 7 days in two groups: 1. In DBM in the microfloid device 2. In DBM in static state (control) , under the following conditions: 1.negative control 2.cytokine 3.nicotinamide 4.nicotinamide+cytokine 5.negative control with feeder 6.cytokine+feeder 7.nicotinamide+feeder 8.nicotinamide+cytokine+feeder After 7 days, cell count, their purity using flow cytometry, Colony forming unit assay and apoptosis were evaluated. The status of the cells on the scaffold was observed using electron microscopy
Results
Our results indicated that more cells were counted in static culture than microfluidic culture (perfusion), while the ability of cell colonization in microfluidic culture was far higher. The purity of HSCs was higher in microfluidic culture, and fewer cells entered the apoptotic phase. In all cases, the combination of nicotinamide and cytokine in the presence of feeder, was accompanied with more proliferation, more CD34 + cells, less apoptosis, and higher colonyforming ability than other groups.
Conclusion
Nicotinamide by preventing epigenetic changes resulting from laboratorial cell culture and microfluidic device associated with DBM by simulating bone marrow niche is a very suitable compound for the development of these cells.
Session topic: 24. Hematopoiesis, stem cells and microenvironment
Keyword(s): Hematopoietic Stem Cell, Mesenchymal stem cell, Stem cell expansion, Stem cell niche