Contributions
Abstract: PB1995
Type: Publication Only
Background
Hodgkin’s lymphoma (HL) is a potentially curable malignant lymphoma with distinct histology, biologic behavior, and clinical characteristics. Epstein Barr- virus (EBV) has remained the main candidate suggested as the infectious agent causing Hodgkin's lymphoma for many years. To our knowledge, in The Sudan, no previous study was performed to associate Hodgkin’s lymphoma to infectious agents. In particular, no data available about the prevalence of EBV in HL, nor the variability among different HL pathological subtypes. It is important to stress that infectious agents are so common in in The Sudan as one of the developing countries.
Aims
To screen the paraffin embedded lymph nodes for the presence of Epstein Barr virus genome in Hodgkin’s lymphoma cases compared to reactive lymphadenitis controls.
Methods
This is a retrospective case control study. All cases of HL were first confirmed histologically and the difficult ones were further immunohistochemically confirmed. Genomic DNA was extracted from formalin fixed paraffin embedded lymph nodes using commercial DNA extraction Kits, followed by PCR amplification of three different genes (LMP 1, EBNA1 and IR3 region), using three different sets of specific primers. Amplified DNA was then visualized after agarose gel electrophoresis.
Results
Forty-one cases were enrolled, 25 (61%) of them were males and 16 (39%) were females with 1.6/1 male to female ratio. The mean of age was 30 years for both males and females. Fifty-nine percent of patients were either children or young adults of less than thirty years old. HL cases were examined for histology and immunohistochemistry and accordingly, classified as 23 (56%) cases of mixed cellularity (MC) (Fig.1), 16 (39%) cases of nodular sclerosis (NS) (Fig.2), one case (2.4%) of each of lymphocyte predominance (LP) and lymphocyte depletion (LD). EBV genome was detected in approximately half (51%) of HL patients (P value = 0.035) and 18.8% of reactive lymphadenitis controls (Fig.3, Fig,4). EBV virus was much more expressed in Mixed cellularity HL subtype compared to other subtypes as the virus was detected in 13 cases of Mixed Cellularity and 7 cases of Nodular Sclerosis (Fig.5).
Conclusion
EBV virus is associated with approximately half of Hodgkin’s lymphoma cases in Sudanese enrolled in this study and particularly associated with the Mixed Cellularity subtype. Further perspective study is highly recommended to investigate the involvement of EBV in pathogenesis of Hodgkin’s lymphoma in Sudanese and whether it can be used for future targeted therapeutics.
Session topic: 18. Hodgkin lymphoma – Biology & Translational Research
Keyword(s): EBV, Hodgkin's Lymphoma
Abstract: PB1995
Type: Publication Only
Background
Hodgkin’s lymphoma (HL) is a potentially curable malignant lymphoma with distinct histology, biologic behavior, and clinical characteristics. Epstein Barr- virus (EBV) has remained the main candidate suggested as the infectious agent causing Hodgkin's lymphoma for many years. To our knowledge, in The Sudan, no previous study was performed to associate Hodgkin’s lymphoma to infectious agents. In particular, no data available about the prevalence of EBV in HL, nor the variability among different HL pathological subtypes. It is important to stress that infectious agents are so common in in The Sudan as one of the developing countries.
Aims
To screen the paraffin embedded lymph nodes for the presence of Epstein Barr virus genome in Hodgkin’s lymphoma cases compared to reactive lymphadenitis controls.
Methods
This is a retrospective case control study. All cases of HL were first confirmed histologically and the difficult ones were further immunohistochemically confirmed. Genomic DNA was extracted from formalin fixed paraffin embedded lymph nodes using commercial DNA extraction Kits, followed by PCR amplification of three different genes (LMP 1, EBNA1 and IR3 region), using three different sets of specific primers. Amplified DNA was then visualized after agarose gel electrophoresis.
Results
Forty-one cases were enrolled, 25 (61%) of them were males and 16 (39%) were females with 1.6/1 male to female ratio. The mean of age was 30 years for both males and females. Fifty-nine percent of patients were either children or young adults of less than thirty years old. HL cases were examined for histology and immunohistochemistry and accordingly, classified as 23 (56%) cases of mixed cellularity (MC) (Fig.1), 16 (39%) cases of nodular sclerosis (NS) (Fig.2), one case (2.4%) of each of lymphocyte predominance (LP) and lymphocyte depletion (LD). EBV genome was detected in approximately half (51%) of HL patients (P value = 0.035) and 18.8% of reactive lymphadenitis controls (Fig.3, Fig,4). EBV virus was much more expressed in Mixed cellularity HL subtype compared to other subtypes as the virus was detected in 13 cases of Mixed Cellularity and 7 cases of Nodular Sclerosis (Fig.5).
Conclusion
EBV virus is associated with approximately half of Hodgkin’s lymphoma cases in Sudanese enrolled in this study and particularly associated with the Mixed Cellularity subtype. Further perspective study is highly recommended to investigate the involvement of EBV in pathogenesis of Hodgkin’s lymphoma in Sudanese and whether it can be used for future targeted therapeutics.
Session topic: 18. Hodgkin lymphoma – Biology & Translational Research
Keyword(s): EBV, Hodgkin's Lymphoma