Contributions
Abstract: PB2266
Type: Publication Only
Background
Myeloproliferative neoplasms Ph-negative (MPN Ph-) are clinically heterogenous group of malignancies, characterized by presence of JAK2 or CALR or MPL mutation in most of the cases. Beside their clinical differences, these neoplasms differ significantly on histological grounds, not only in hematopoietic cells morphology and architecture, but also their microenviroment features. Mesenchymal stromal cells (MSCs) CD271-positive play a major role in hematopoietic stem cells renewal, and have been analysed previously in context of solid tumor propagation and some hematological cancers development
Aims
to analyse quantity and architecture of CD271-postive MSCs in MPN Ph-
Methods
We have analysed bone marrow trephine biopsies from 22 MPN Ph- patients: essential thrombocythemia (ET; n=7), polycythemia vera (PV; n=3), myelofibrosis (MF; n=11), prefibrotic myelofibrosis (prePMF; n=1) and 4 with non MPN diagnosis: chronic lymphocytic leukemia (n=1) and reactive disorders (n=3). Clinical, analytical and molecular data were collected. Histological sections were stained immunohistochemically using anti-CD271 antibody. Extension of staining was assesed semiquantitatively in a 3-grade (1-3) manner and correlated with diagnosis and several clinical and laboratory parameters.
Results
Here we present a very preliminary results. The CD271 staining grade 3 was observed in 6 from 11 MF (54.5%), grade 2 was observed in 5 from 11 MF (45.5%) and in 1 from 1 prePMF. The CD271 staining grade 1 was observed only in 1 MF and in all PV, ET and in non-MPN patients. There was no significant relation between CD271 and mutational status (JAK2, CALR), white blood cells, platelet counts, haemoglobin level, LDH level. There was no significant relation between CD271 and IPSS, DIPSS and DIPSS plus risk score.
Conclusion
Although we have analyzed a very small group of patients, a strong relationship between MF and high CD271+ cells density in bone marrow is visible. It surely requires further investigations, but CD271+ cells density assessment might be helpful in diagnosis of high-grade MPNs. It may also show novel insight into MPNs development and pathology.
Session topic: 15. Myeloproliferative neoplasms – Biology & Translational Research
Keyword(s): Mesenchymal stem cell, Microenvironment, Myelofibrosis, Myeloproliferative disorder
Abstract: PB2266
Type: Publication Only
Background
Myeloproliferative neoplasms Ph-negative (MPN Ph-) are clinically heterogenous group of malignancies, characterized by presence of JAK2 or CALR or MPL mutation in most of the cases. Beside their clinical differences, these neoplasms differ significantly on histological grounds, not only in hematopoietic cells morphology and architecture, but also their microenviroment features. Mesenchymal stromal cells (MSCs) CD271-positive play a major role in hematopoietic stem cells renewal, and have been analysed previously in context of solid tumor propagation and some hematological cancers development
Aims
to analyse quantity and architecture of CD271-postive MSCs in MPN Ph-
Methods
We have analysed bone marrow trephine biopsies from 22 MPN Ph- patients: essential thrombocythemia (ET; n=7), polycythemia vera (PV; n=3), myelofibrosis (MF; n=11), prefibrotic myelofibrosis (prePMF; n=1) and 4 with non MPN diagnosis: chronic lymphocytic leukemia (n=1) and reactive disorders (n=3). Clinical, analytical and molecular data were collected. Histological sections were stained immunohistochemically using anti-CD271 antibody. Extension of staining was assesed semiquantitatively in a 3-grade (1-3) manner and correlated with diagnosis and several clinical and laboratory parameters.
Results
Here we present a very preliminary results. The CD271 staining grade 3 was observed in 6 from 11 MF (54.5%), grade 2 was observed in 5 from 11 MF (45.5%) and in 1 from 1 prePMF. The CD271 staining grade 1 was observed only in 1 MF and in all PV, ET and in non-MPN patients. There was no significant relation between CD271 and mutational status (JAK2, CALR), white blood cells, platelet counts, haemoglobin level, LDH level. There was no significant relation between CD271 and IPSS, DIPSS and DIPSS plus risk score.
Conclusion
Although we have analyzed a very small group of patients, a strong relationship between MF and high CD271+ cells density in bone marrow is visible. It surely requires further investigations, but CD271+ cells density assessment might be helpful in diagnosis of high-grade MPNs. It may also show novel insight into MPNs development and pathology.
Session topic: 15. Myeloproliferative neoplasms – Biology & Translational Research
Keyword(s): Mesenchymal stem cell, Microenvironment, Myelofibrosis, Myeloproliferative disorder