Contributions
Abstract: PB2132
Type: Publication Only
Background
Monoclonal Gammopathies (MG) are plasma cell (PC) dyscrasias caused by clonal accumulation of PC in the bone marrow (BM). The BM microenvironment plays a crucial role in disease development. BM Mesenchymal Stromal Cells (MSC) are able to interact with clonal PC contributing for a microenvironment suitable for disease progression.
Aims
To evaluate the relationship between PC dyscrasias progression with the frequency and phenotype of BM MSC.
Methods
Clinical process consultation and BM aspirates were proceded in patients diagnosed with MGUS (n=32), smoldering Multiple Myeloma (sMM) (n=5) and MM (n=24), and in a control group (n=10) of normal BM samples. Multidimensional flow cytometry was used to identify, quantify and characterize BM MSC (based on the expression of CD13, CD24, CD29, CD49e, CD73, CD90, CD105, CD106 and CD271).
Results
After frequency normalization, by removing clonal PCs from all BM nucleated cells, a significantly increased in the frequency of MSC (0,137%) was observed in symptomatic MM patients when compared with sMM (0,027%), MGUS (0,027%) and normal BM (0,034%). We also found that the higher frequency of BM MSC was associated with lower hemoglobin concentrations, higher frequency of BM PC and higher ISS staging. In MM patients, a higher MSC frequency was observed in the cases whereas clonal PC didn´t express CD56. Moreover, a lower expression of CD73 and a higher of CD90 by MSC, was observed through disease progression, from MGUS to MM.
Conclusion
The higher frequency of MSC and the altered phenotype observed in BM samples from patients with PC dyscrasias, could play an important role in MM pathogenesis.
Session topic: 13. Myeloma and other monoclonal gammopathies – Biology & Translational Research
Keyword(s): Mesenchymal cells, Monoclonal gammopathy, Myeloma
Abstract: PB2132
Type: Publication Only
Background
Monoclonal Gammopathies (MG) are plasma cell (PC) dyscrasias caused by clonal accumulation of PC in the bone marrow (BM). The BM microenvironment plays a crucial role in disease development. BM Mesenchymal Stromal Cells (MSC) are able to interact with clonal PC contributing for a microenvironment suitable for disease progression.
Aims
To evaluate the relationship between PC dyscrasias progression with the frequency and phenotype of BM MSC.
Methods
Clinical process consultation and BM aspirates were proceded in patients diagnosed with MGUS (n=32), smoldering Multiple Myeloma (sMM) (n=5) and MM (n=24), and in a control group (n=10) of normal BM samples. Multidimensional flow cytometry was used to identify, quantify and characterize BM MSC (based on the expression of CD13, CD24, CD29, CD49e, CD73, CD90, CD105, CD106 and CD271).
Results
After frequency normalization, by removing clonal PCs from all BM nucleated cells, a significantly increased in the frequency of MSC (0,137%) was observed in symptomatic MM patients when compared with sMM (0,027%), MGUS (0,027%) and normal BM (0,034%). We also found that the higher frequency of BM MSC was associated with lower hemoglobin concentrations, higher frequency of BM PC and higher ISS staging. In MM patients, a higher MSC frequency was observed in the cases whereas clonal PC didn´t express CD56. Moreover, a lower expression of CD73 and a higher of CD90 by MSC, was observed through disease progression, from MGUS to MM.
Conclusion
The higher frequency of MSC and the altered phenotype observed in BM samples from patients with PC dyscrasias, could play an important role in MM pathogenesis.
Session topic: 13. Myeloma and other monoclonal gammopathies – Biology & Translational Research
Keyword(s): Mesenchymal cells, Monoclonal gammopathy, Myeloma