Contributions
Abstract: PB2112
Type: Publication Only
Background
Myelodysplastic syndromes MDS is a hematopoietic disorder of clonal pluripotent stem cells, bone marrow failure, varying degree of pancytopenia and a propensity for conversion to Acute Myeloid Leukemia. The myeloid lineage is mostly involved but there is ongoing immune responses occurs in MDS involving B cell with or without T cell involvement. Studies suggests an increasing evidence of an autoimmune process in pathogenesis of the bone marrow failure that accompanies MDS. Analysis of CD4/CD8 subsets analysis shows abnormal expansion in MDS and could predict response to treatment.
Aims
The current has been done to evaluate the intimacy of t cells in the pathogenesis of MDS.
Methods
The study was done at National Institute of Blood Diseases and Bone Marrow Transplantation, Karachi Pakistan during the period of June 2017 to January 2018.A total of 12 MDS including (7 males and 5 females) confirmed patients meeting criteria of WHO classification were included.24 aged and sex matched were also part of study. Approval for the study was obtained from the Institutional Review Board of NIBD. Written informed consent of each participant was also obtained. However, the illiterate participants had the consent forms read and interpreted to them in their native languages and with their oral consents, literate family representatives of the illiterate participants signed the consent forms on behalf of each of them.In this cross-sectional study, using sterile disposable needles and syringes, 5 ml of blood was aseptically collected from each participant by venipuncture of the cubital vein. Samples were each placed in BD vacutainers containing anticoagulant, and conveyed to the Flow Cytometry and Hematology Departments.CBC was done utilizing XN-1000.CD3,CD4 and CD8 T cells were enumerated using BD FACScount flow cytometer (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions.
Results
A total of 12 MDS patients with 24 aged and gender matched controls were included. The mean age of the patients was 43yrs±18.4.The common presenting complain was weakness, fever in 8(66.6%) patient followed by abdominal pain and gums bleeding in 4(33.3%) patients. The mean Hb of patients was 8.71±2.05(g/dl),TLC 4.39±2.03*10^9/l and platelets count 132±168*10^9/l with ipss of o.8 while in control group Hb 14.24±1.29(g/dl) ,TLC 7.18±1.87(*10^9/l) and platelets counts were 231±68(*10^9/l).In patient CD4 counts were 693±426 and elevated CD8 were 620±313.8 while in control group CD4 counts were 858±355 and CD8 counts 537±208.
Conclusion
MDS is although a disease of bone marrow failure but there is an strong evidence that autoimmune processes are involment in the pathogenesis of bone marrow failure mds..Further studies with large sample size are needed to determine whether CD4/CD8 T cells analysis could be useful in predicting responders to immunosuppressive treatment.
Session topic: 10. Myelodysplastic syndromes – Clinical
Keyword(s): CD4+ T cells, CD8 T cells, Myelodysplasia
Abstract: PB2112
Type: Publication Only
Background
Myelodysplastic syndromes MDS is a hematopoietic disorder of clonal pluripotent stem cells, bone marrow failure, varying degree of pancytopenia and a propensity for conversion to Acute Myeloid Leukemia. The myeloid lineage is mostly involved but there is ongoing immune responses occurs in MDS involving B cell with or without T cell involvement. Studies suggests an increasing evidence of an autoimmune process in pathogenesis of the bone marrow failure that accompanies MDS. Analysis of CD4/CD8 subsets analysis shows abnormal expansion in MDS and could predict response to treatment.
Aims
The current has been done to evaluate the intimacy of t cells in the pathogenesis of MDS.
Methods
The study was done at National Institute of Blood Diseases and Bone Marrow Transplantation, Karachi Pakistan during the period of June 2017 to January 2018.A total of 12 MDS including (7 males and 5 females) confirmed patients meeting criteria of WHO classification were included.24 aged and sex matched were also part of study. Approval for the study was obtained from the Institutional Review Board of NIBD. Written informed consent of each participant was also obtained. However, the illiterate participants had the consent forms read and interpreted to them in their native languages and with their oral consents, literate family representatives of the illiterate participants signed the consent forms on behalf of each of them.In this cross-sectional study, using sterile disposable needles and syringes, 5 ml of blood was aseptically collected from each participant by venipuncture of the cubital vein. Samples were each placed in BD vacutainers containing anticoagulant, and conveyed to the Flow Cytometry and Hematology Departments.CBC was done utilizing XN-1000.CD3,CD4 and CD8 T cells were enumerated using BD FACScount flow cytometer (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions.
Results
A total of 12 MDS patients with 24 aged and gender matched controls were included. The mean age of the patients was 43yrs±18.4.The common presenting complain was weakness, fever in 8(66.6%) patient followed by abdominal pain and gums bleeding in 4(33.3%) patients. The mean Hb of patients was 8.71±2.05(g/dl),TLC 4.39±2.03*10^9/l and platelets count 132±168*10^9/l with ipss of o.8 while in control group Hb 14.24±1.29(g/dl) ,TLC 7.18±1.87(*10^9/l) and platelets counts were 231±68(*10^9/l).In patient CD4 counts were 693±426 and elevated CD8 were 620±313.8 while in control group CD4 counts were 858±355 and CD8 counts 537±208.
Conclusion
MDS is although a disease of bone marrow failure but there is an strong evidence that autoimmune processes are involment in the pathogenesis of bone marrow failure mds..Further studies with large sample size are needed to determine whether CD4/CD8 T cells analysis could be useful in predicting responders to immunosuppressive treatment.
Session topic: 10. Myelodysplastic syndromes – Clinical
Keyword(s): CD4+ T cells, CD8 T cells, Myelodysplasia