Contributions
Abstract: PB2097
Type: Publication Only
Background
Depletion of progenitors B cells (PBC) has been described as a frequent event in myelodysplastic syndromes and aplastic anemia, but we do not know if this event occurs in other related conditions as chronic myelomonocytic leukemia (CMML), and if it has any potential diagnostic or prognostic significance.
Aims
Describe the prevalence of depletion of PBC at bone marrow of CMML at diagnosis, and correlate if exists difference in the progression-free survival (PFS) compared to patients without this event.
Methods
We retrospectively reviewed the bone marrow flow cytometry of CMML patients at diagnosis during the period of 2008-2016. Depletion of PBC (CD34+CD19+CD10+) was defined as a value less than 0.05% of the total nucleated cell at the bone marrow. PFS was defined as the time from diagnosis to progression to acute myeloid leukemia.
Results
The studied group had a median age of 73.1 years, with a distribution by sex (male/female) of 61% and 49% respectively, and a mean follow-up of 24.2 months. According to WHO subtype (0, 1 and 2), the distribution was 66.7%, 11.1% and 22.2%, respectively. We found that 78% of the patients had depletion of PBC. The depleted patients had the CPSS prognostic scale (low/int-1/int-2/high) of 23%, 15.3%, 46.1% and 15.3%, and the non-depleted of 20%, 20%, 60%, 0%, respectively. By FAB subtype the proportion of dysplastic versus proliferative disease was 46% and. 54% in depleted patients, and 60% and 40% in non-depleted. The PFS to 2 years was 50% in non-depleted patients versus 46% in depleted patients (p: 0.816, see figure 1).
Conclusion
Based in our data, it is possible to consider that the depletion of PBC as a frequent event in CMML, and there is not a prognostic correlation with statistical significance in terms of PFS to 2 years in depleted or non-depleted patients. The depletion of PBC occurred without specific tendency in all subgroups by CPSS and FAB classification. Due to small sample size, further studies are necessary to clarify the relevance of this event.
Session topic: 10. Myelodysplastic syndromes – Clinical
Keyword(s): Chronic myelomonocytic leukemia, flow cytometry, Progenitor cell
Abstract: PB2097
Type: Publication Only
Background
Depletion of progenitors B cells (PBC) has been described as a frequent event in myelodysplastic syndromes and aplastic anemia, but we do not know if this event occurs in other related conditions as chronic myelomonocytic leukemia (CMML), and if it has any potential diagnostic or prognostic significance.
Aims
Describe the prevalence of depletion of PBC at bone marrow of CMML at diagnosis, and correlate if exists difference in the progression-free survival (PFS) compared to patients without this event.
Methods
We retrospectively reviewed the bone marrow flow cytometry of CMML patients at diagnosis during the period of 2008-2016. Depletion of PBC (CD34+CD19+CD10+) was defined as a value less than 0.05% of the total nucleated cell at the bone marrow. PFS was defined as the time from diagnosis to progression to acute myeloid leukemia.
Results
The studied group had a median age of 73.1 years, with a distribution by sex (male/female) of 61% and 49% respectively, and a mean follow-up of 24.2 months. According to WHO subtype (0, 1 and 2), the distribution was 66.7%, 11.1% and 22.2%, respectively. We found that 78% of the patients had depletion of PBC. The depleted patients had the CPSS prognostic scale (low/int-1/int-2/high) of 23%, 15.3%, 46.1% and 15.3%, and the non-depleted of 20%, 20%, 60%, 0%, respectively. By FAB subtype the proportion of dysplastic versus proliferative disease was 46% and. 54% in depleted patients, and 60% and 40% in non-depleted. The PFS to 2 years was 50% in non-depleted patients versus 46% in depleted patients (p: 0.816, see figure 1).
Conclusion
Based in our data, it is possible to consider that the depletion of PBC as a frequent event in CMML, and there is not a prognostic correlation with statistical significance in terms of PFS to 2 years in depleted or non-depleted patients. The depletion of PBC occurred without specific tendency in all subgroups by CPSS and FAB classification. Due to small sample size, further studies are necessary to clarify the relevance of this event.
Session topic: 10. Myelodysplastic syndromes – Clinical
Keyword(s): Chronic myelomonocytic leukemia, flow cytometry, Progenitor cell