Contributions
Abstract: PB2084
Type: Publication Only
Background
MDS is a clonal disorder which patients have pancytopenia, anemia and transfusion dependent. This disorder progress to AML in 1:3 of cases and resistant to chemotherapy. Several molecular mechanisms involved in pathogenesis of MDS including hypermethylation of selected genes in different manner like apoptosis, cell cycle, and tumor suppressor.
Aims
In this work we studied methylation and epigenetic of several genes including FOXO3, CHK2, HRK and PTEN involved in apoptosis and relationship to biological characteristic and karyotype and subgroup of MDS and IPSS.
Methods
We studied 54 patients enrolled in Shariati Hospital , Firozgar hospital and other therapuetic center in Tehran. PB or BM were collected and DNA and RNA extracted. DNA was bisulfitted, methylated and measured by MS-HRM. cDNA synthesized from RNA and expression of genes studied by REAL TIME PCR. SPSS 16 software was used to analyse the data.
Results
The most frequency of methylation occurred in CHK2 gene which has been seen in all subtypes of MDS including RAEB1, 2. The advanced stage of MDS showed the most hypermethylation status compare to early PCR stage. The most hypermethylation of gene occurred in high and very high subgroup of IPSS-R. Real time showed downregulation of selected genes which were in parallel to hypermethylation pattern.
Conclusion
The data showed hypermethylation of all selected genes inviolve in apoptosis could explain the mechanisms and pathogenesis in MDS in partly . This hypermethylation was parallel to decease in expression of selected genes.
Session topic: 9. Myelodysplastic syndromes – Biology & Translational Research
Keyword(s): Apoptosis, Hypermethylation, MDS
Abstract: PB2084
Type: Publication Only
Background
MDS is a clonal disorder which patients have pancytopenia, anemia and transfusion dependent. This disorder progress to AML in 1:3 of cases and resistant to chemotherapy. Several molecular mechanisms involved in pathogenesis of MDS including hypermethylation of selected genes in different manner like apoptosis, cell cycle, and tumor suppressor.
Aims
In this work we studied methylation and epigenetic of several genes including FOXO3, CHK2, HRK and PTEN involved in apoptosis and relationship to biological characteristic and karyotype and subgroup of MDS and IPSS.
Methods
We studied 54 patients enrolled in Shariati Hospital , Firozgar hospital and other therapuetic center in Tehran. PB or BM were collected and DNA and RNA extracted. DNA was bisulfitted, methylated and measured by MS-HRM. cDNA synthesized from RNA and expression of genes studied by REAL TIME PCR. SPSS 16 software was used to analyse the data.
Results
The most frequency of methylation occurred in CHK2 gene which has been seen in all subtypes of MDS including RAEB1, 2. The advanced stage of MDS showed the most hypermethylation status compare to early PCR stage. The most hypermethylation of gene occurred in high and very high subgroup of IPSS-R. Real time showed downregulation of selected genes which were in parallel to hypermethylation pattern.
Conclusion
The data showed hypermethylation of all selected genes inviolve in apoptosis could explain the mechanisms and pathogenesis in MDS in partly . This hypermethylation was parallel to decease in expression of selected genes.
Session topic: 9. Myelodysplastic syndromes – Biology & Translational Research
Keyword(s): Apoptosis, Hypermethylation, MDS