Contributions
Abstract: PB1855
Type: Publication Only
Background
The diagnostic term monoclonal B-cell lymphocytosis (MBL) is used to characterise individuals with a circulating population of clonal B-cells, a total count of <5 x 10(9)/L, with no other features of a B-cell lymphoproliferative disorder. In the recent literature, it has been reported that circulating chronic lymphocytic leukemia (CLL) - like B cells can be detected using multicolor flow cytometry in 0.57%>12% of adults with normal lymphocyte counts.
Aims
As far as we know, this is the first study in Greek population, investigating the frequency of B-cell lymphocytosis in healthy individuals, trying to understand the prognostic factors that lead the occurence of MBL to evove into B-CLL.
Methods
We investigate the frequency of circulating monoclonal B cells in 815 healthy blood donors aged 30-70 years with no evident history of malignant disease and normal blood counts, who provided signed informed consent. We used flow cytometric analysis of CD19/CD5/CD79b/CD20/CD23/CD38 expression, while other parameters such as LDH, beta-2 microglobulin and C reactive protein were measured. Monoclonality was demonstrated by immunoglobulin light-chain restriction in all cases with CLL phenotype cells.
Results
The monoclonal CLL phenotype cells were detected in 1.6% of individuals, with a higher frequency in men (male-to-female ratio, 1.2:1) and elderly individuals (1.9% of 40- to 59-year-olds versus 4.0% of 60- to 89-year olds, P=0.01) which is within the framework of international studies.
Conclusion
Our study shows that a healthy percentage of the population in Greece, equal to 1.6% is bearing cells with CLL immunophenotype. The use of more extensive immunophenotype-order control or control of clonality seems to be a valuable prognostic factor for the occurence of monoclonal lymphocytosis, which may potentially evolve into B-CLL over time. Furthermore it is of great importance monitoring of these patients over time.
Session topic: 5. Chronic lymphocytic leukemia and related disorders – Biology & Translational Research
Keyword(s): B cell chronic lymphocytic leukemia, Cytometry, Immunophenotype, Lymphocyte
Abstract: PB1855
Type: Publication Only
Background
The diagnostic term monoclonal B-cell lymphocytosis (MBL) is used to characterise individuals with a circulating population of clonal B-cells, a total count of <5 x 10(9)/L, with no other features of a B-cell lymphoproliferative disorder. In the recent literature, it has been reported that circulating chronic lymphocytic leukemia (CLL) - like B cells can be detected using multicolor flow cytometry in 0.57%>12% of adults with normal lymphocyte counts.
Aims
As far as we know, this is the first study in Greek population, investigating the frequency of B-cell lymphocytosis in healthy individuals, trying to understand the prognostic factors that lead the occurence of MBL to evove into B-CLL.
Methods
We investigate the frequency of circulating monoclonal B cells in 815 healthy blood donors aged 30-70 years with no evident history of malignant disease and normal blood counts, who provided signed informed consent. We used flow cytometric analysis of CD19/CD5/CD79b/CD20/CD23/CD38 expression, while other parameters such as LDH, beta-2 microglobulin and C reactive protein were measured. Monoclonality was demonstrated by immunoglobulin light-chain restriction in all cases with CLL phenotype cells.
Results
The monoclonal CLL phenotype cells were detected in 1.6% of individuals, with a higher frequency in men (male-to-female ratio, 1.2:1) and elderly individuals (1.9% of 40- to 59-year-olds versus 4.0% of 60- to 89-year olds, P=0.01) which is within the framework of international studies.
Conclusion
Our study shows that a healthy percentage of the population in Greece, equal to 1.6% is bearing cells with CLL immunophenotype. The use of more extensive immunophenotype-order control or control of clonality seems to be a valuable prognostic factor for the occurence of monoclonal lymphocytosis, which may potentially evolve into B-CLL over time. Furthermore it is of great importance monitoring of these patients over time.
Session topic: 5. Chronic lymphocytic leukemia and related disorders – Biology & Translational Research
Keyword(s): B cell chronic lymphocytic leukemia, Cytometry, Immunophenotype, Lymphocyte