Contributions
Abstract: PB1708
Type: Publication Only
Background
Acute myeloblastic leukemia is a severe onco-hematologic disease. The functioning of the immune system depends on the characteristics and severity of metabolic processes in its cells. Enzymes of oxidation-reduction reactions are used to evaluate biochemical processes in lymphocytes, because they realize the basic reactions of intracellular metabolism and are responsible for combining all metabolic exchanges.
Aims
The purpose of this study was to study the dynamics of the mechanisms of metabolic status of lymphocytes in the blood in patients with acute myeloblastic leukemia (AML).
Methods
In the study group there were 100 AML patients, in the acute debut stage - 30 patients, in the stage of complete remission after the treatment - 49 patients, in the stage of repeated relapse - 21 people. The control group consisted of 125 practically healthy volunteers. Bioluminescent determination of the activity of NAD (P) -dependent dehydrogenases was carried out on the biochemiluminometer BLM-3607. This method was used to determine the activity of the following enzymes: glucose-6-phosphate dehydrogenase, glycerol-3-phosphate dehydrogenase, malic enzyme, NAD- and NADH-dependent lactate dehydrogenase reaction, NAD- and NADH-dependent reaction of malate dehydrogenase, NADP- and NADPH-dependent glutamate dehydrogenase, NAD - and NADH-dependent glutamate dehydrogenase, NAD- and NADP-dependent isocitrate dehydrogenases and glutathione reductase. Activity of dehydrogenases in blood lymphocytes was expressed in enzymatic units (1 E = 1 μmol / min) per 104 cells).
Based on the results of the research, a database was created in MS Excel 2000 spreadsheets, on the basis of which statistical analysis was carried out using descriptive statistics methods with Student's t-criterion, nonparametric using exact Mann-Whitney criteria using the Statistica 8.0 application software package.
Results
When studying the features of the metabolic status of blood lymphocytes in patients with a primary AML attack, a decrease in the activity of LDH aerobic reaction is revealed. The decrease in the intensity of terminal glycolysis reactions and the level of concentration of intermediates for the Krebs cycle is proved by the revealed decreased activity of LDH and NADH-LDH. The level of reaction of the Krebs cycle of NADP-dependent isocitrate dehydrogenase in patients at the acute debut stage relative to control is decreased. In patients in the stage of complete remission of AML, the activity of the NADH-LDH enzyme is restored, while the activity of the LDH enzyme remains reduced. When studying the metabolic status of patients with the M2-variant AML in a repeated relapse, a decrease in the transfer of lipid catabolism products to the redox-oxidative reactions of glycolysis is revealed, due to a decrease in the activity of G3PDH. The decrease in glycolysis activity leads to its inhibition, which is confirmed by the decreased activity of the anaerobic LDH reaction. The activity of NADH-LDH is decreased. The activity of aerobic LDH reaction in lymphocytes was reduced. However, in patients with AML at the stage of recurrence, the activity of glutathione reductase is restored.
Conclusion
At all stages of AML, a marked decrease in the intensity of intracellular metabolic processes of lymphocytes is observed, mitochondrial transport, glycolysis, and metabolism in the lemon cycle decrease. A feature of acute debut and remission of AML is the reduction of peroxide processes in lymphocytes. With repeated relapse, peroxide processes in lymphocytes are restored; however, intracellular lipid catabolism decreases.
Session topic: 3. Acute myeloid leukemia - Biology & Translational Research
Keyword(s): Tumorigenesis
Abstract: PB1708
Type: Publication Only
Background
Acute myeloblastic leukemia is a severe onco-hematologic disease. The functioning of the immune system depends on the characteristics and severity of metabolic processes in its cells. Enzymes of oxidation-reduction reactions are used to evaluate biochemical processes in lymphocytes, because they realize the basic reactions of intracellular metabolism and are responsible for combining all metabolic exchanges.
Aims
The purpose of this study was to study the dynamics of the mechanisms of metabolic status of lymphocytes in the blood in patients with acute myeloblastic leukemia (AML).
Methods
In the study group there were 100 AML patients, in the acute debut stage - 30 patients, in the stage of complete remission after the treatment - 49 patients, in the stage of repeated relapse - 21 people. The control group consisted of 125 practically healthy volunteers. Bioluminescent determination of the activity of NAD (P) -dependent dehydrogenases was carried out on the biochemiluminometer BLM-3607. This method was used to determine the activity of the following enzymes: glucose-6-phosphate dehydrogenase, glycerol-3-phosphate dehydrogenase, malic enzyme, NAD- and NADH-dependent lactate dehydrogenase reaction, NAD- and NADH-dependent reaction of malate dehydrogenase, NADP- and NADPH-dependent glutamate dehydrogenase, NAD - and NADH-dependent glutamate dehydrogenase, NAD- and NADP-dependent isocitrate dehydrogenases and glutathione reductase. Activity of dehydrogenases in blood lymphocytes was expressed in enzymatic units (1 E = 1 μmol / min) per 104 cells).
Based on the results of the research, a database was created in MS Excel 2000 spreadsheets, on the basis of which statistical analysis was carried out using descriptive statistics methods with Student's t-criterion, nonparametric using exact Mann-Whitney criteria using the Statistica 8.0 application software package.
Results
When studying the features of the metabolic status of blood lymphocytes in patients with a primary AML attack, a decrease in the activity of LDH aerobic reaction is revealed. The decrease in the intensity of terminal glycolysis reactions and the level of concentration of intermediates for the Krebs cycle is proved by the revealed decreased activity of LDH and NADH-LDH. The level of reaction of the Krebs cycle of NADP-dependent isocitrate dehydrogenase in patients at the acute debut stage relative to control is decreased. In patients in the stage of complete remission of AML, the activity of the NADH-LDH enzyme is restored, while the activity of the LDH enzyme remains reduced. When studying the metabolic status of patients with the M2-variant AML in a repeated relapse, a decrease in the transfer of lipid catabolism products to the redox-oxidative reactions of glycolysis is revealed, due to a decrease in the activity of G3PDH. The decrease in glycolysis activity leads to its inhibition, which is confirmed by the decreased activity of the anaerobic LDH reaction. The activity of NADH-LDH is decreased. The activity of aerobic LDH reaction in lymphocytes was reduced. However, in patients with AML at the stage of recurrence, the activity of glutathione reductase is restored.
Conclusion
At all stages of AML, a marked decrease in the intensity of intracellular metabolic processes of lymphocytes is observed, mitochondrial transport, glycolysis, and metabolism in the lemon cycle decrease. A feature of acute debut and remission of AML is the reduction of peroxide processes in lymphocytes. With repeated relapse, peroxide processes in lymphocytes are restored; however, intracellular lipid catabolism decreases.
Session topic: 3. Acute myeloid leukemia - Biology & Translational Research
Keyword(s): Tumorigenesis