Contributions
Abstract: PB1707
Type: Publication Only
Background
Acute myeloblastic leukemia is a severe onco-hematologic disease. Reducing the reactivity of the patient's immune system is one of the provocative mechanisms in its progression.
Aims
The purpose of this study was to study the dynamics of the subpopulation composition of lymphocytes in the blood in patients with acute myeloblastic leukemia (AML).
Methods
In the study group there were 100 AML patients, in the acute debut stage - 30 patients, in the stage of complete remission after the treatment - 49 patients, in the stage of repeated relapse - 21 people. For the stage of acute debut, the first manifestation of the disease with the number of blasts in the bone marrow is 25% or more. Complete remission after treatment in patients was diagnosed with detecting in the myelogram of not more than 5% of blast cells with normal cellularity, in the cerebrospinal fluid there were no leukemia cells. Repeated relapse was detected when more than 25% of the blasts were detected in the bone marrow indices after the remission achieved before. The control group consisted of 125 practically healthy volunteers. BD MultiTEST test systems with a 4-color antibody panel: CD3 / CD8 / CD45 / CD4 and CD3 / CD16 + CD56 / CD19, labeled with fluorochromes FITC, PE, PerCP and APC, were used to estimate the relative content of lymphocyte subpopulations. To calculate the absolute content of subpopulations, the absolute number of lymphocytes obtained from the blood test on the hematological analyzer Cell Dyn1800 (Abbott, USA) was used. Student t-test, non-parametric Mann-Whitney criterion U, and linear correlation coefficient r were used for statistical data processing.
Results
The state of the cell link of immunity in patients with the M2 variant of AML during primary attack is characterized by a decrease in the relative number of pan-markers of T-lymphocytes and T-helpers, which indicates not only the development of an immunodeficiency state, but also the inferiority of the T-helper link, leading to inefficiency and cellular, and humoral units of immunity. At the stage of complete remission, leukopenia, a decrease in the relative and absolute indices of the T-cell link of immunity, NK cells, and B-lymphocytes with violation of activation processes are revealed. With repeated relapse of AML, the state of cellular immunity manifests itself in the form of leukopenia, lymphopenia, a decrease in the relative and absolute indices of the T-cell link of immunity, NK cells, and B-lymphocytes with disruption of activation processes.
Conclusion
At the stage of acute debut and remission of AML, T-cell immunodeficiency develops, at the stage of repeated relapse - combined immunodeficiency with defeat of T- and B-systems of immunity. A feature of AML is the depletion of NK cells, which contributes to the progression of the disease and the development of relapse.
Session topic: 3. Acute myeloid leukemia - Biology & Translational Research
Abstract: PB1707
Type: Publication Only
Background
Acute myeloblastic leukemia is a severe onco-hematologic disease. Reducing the reactivity of the patient's immune system is one of the provocative mechanisms in its progression.
Aims
The purpose of this study was to study the dynamics of the subpopulation composition of lymphocytes in the blood in patients with acute myeloblastic leukemia (AML).
Methods
In the study group there were 100 AML patients, in the acute debut stage - 30 patients, in the stage of complete remission after the treatment - 49 patients, in the stage of repeated relapse - 21 people. For the stage of acute debut, the first manifestation of the disease with the number of blasts in the bone marrow is 25% or more. Complete remission after treatment in patients was diagnosed with detecting in the myelogram of not more than 5% of blast cells with normal cellularity, in the cerebrospinal fluid there were no leukemia cells. Repeated relapse was detected when more than 25% of the blasts were detected in the bone marrow indices after the remission achieved before. The control group consisted of 125 practically healthy volunteers. BD MultiTEST test systems with a 4-color antibody panel: CD3 / CD8 / CD45 / CD4 and CD3 / CD16 + CD56 / CD19, labeled with fluorochromes FITC, PE, PerCP and APC, were used to estimate the relative content of lymphocyte subpopulations. To calculate the absolute content of subpopulations, the absolute number of lymphocytes obtained from the blood test on the hematological analyzer Cell Dyn1800 (Abbott, USA) was used. Student t-test, non-parametric Mann-Whitney criterion U, and linear correlation coefficient r were used for statistical data processing.
Results
The state of the cell link of immunity in patients with the M2 variant of AML during primary attack is characterized by a decrease in the relative number of pan-markers of T-lymphocytes and T-helpers, which indicates not only the development of an immunodeficiency state, but also the inferiority of the T-helper link, leading to inefficiency and cellular, and humoral units of immunity. At the stage of complete remission, leukopenia, a decrease in the relative and absolute indices of the T-cell link of immunity, NK cells, and B-lymphocytes with violation of activation processes are revealed. With repeated relapse of AML, the state of cellular immunity manifests itself in the form of leukopenia, lymphopenia, a decrease in the relative and absolute indices of the T-cell link of immunity, NK cells, and B-lymphocytes with disruption of activation processes.
Conclusion
At the stage of acute debut and remission of AML, T-cell immunodeficiency develops, at the stage of repeated relapse - combined immunodeficiency with defeat of T- and B-systems of immunity. A feature of AML is the depletion of NK cells, which contributes to the progression of the disease and the development of relapse.
Session topic: 3. Acute myeloid leukemia - Biology & Translational Research