Contributions
Abstract: PB1670
Type: Publication Only
Background
The purpose of study is to detect change of FAK and PAK1, thus to observe the regulation of the STAT-5 signal pathway in the acute myeloid leukemia cell line (U937) which is managed by the bromo domain inhibitors-JQ1.
Aims
The purpose of study is to detect change of FAK and PAK1, thus to observe the regulation of the STAT-5 signal pathway in the acute myeloid leukemia cell line (U937) which is managed by the bromo domain inhibitors-JQ1.
Methods
With the different concentration of JQ1 to treat U937 cells, the inhibition rate of cells was detected at different times by using tetramethazolazole blue experiment (MTT). The rate of Cell apoptosis was detected by flow cytometry. The mRNA expression of level about FAK and PAK1 were detected by real-time fluorescence quantitative (rt-PCR). And the expression of stat-5 protein which was analyzed by Western Blot in each group
Results
JQ1 can effectively inhibit the proliferation of cells and induce cellular apoptosis, and the apoptosis rate of cells is significantly much more obvious in the high group than the low one. This feature show that a time-dose dependence. JQ1 can also reduce the expression of mRNA about FKA and PAK1 with U937 cell, whenever the concentration of it. The result of Western Blot show that the abnormal expression of STAT-5 in the control group was much more higher than that in the experimental group.
Conclusion
JQ1 can effectively inhibit the growth of U937 cells by influenting proliferation and cell apoptosis. Its effect mechanism may be through to influent the expression of FAK and PAK1, thus to influent the STAT - 5 signal pathway (the pathway of cell proliferation).
Session topic: 3. Acute myeloid leukemia - Biology & Translational Research
Keyword(s): Cell line, STAT5, Therapy, Acute monoblastic leukemia
Abstract: PB1670
Type: Publication Only
Background
The purpose of study is to detect change of FAK and PAK1, thus to observe the regulation of the STAT-5 signal pathway in the acute myeloid leukemia cell line (U937) which is managed by the bromo domain inhibitors-JQ1.
Aims
The purpose of study is to detect change of FAK and PAK1, thus to observe the regulation of the STAT-5 signal pathway in the acute myeloid leukemia cell line (U937) which is managed by the bromo domain inhibitors-JQ1.
Methods
With the different concentration of JQ1 to treat U937 cells, the inhibition rate of cells was detected at different times by using tetramethazolazole blue experiment (MTT). The rate of Cell apoptosis was detected by flow cytometry. The mRNA expression of level about FAK and PAK1 were detected by real-time fluorescence quantitative (rt-PCR). And the expression of stat-5 protein which was analyzed by Western Blot in each group
Results
JQ1 can effectively inhibit the proliferation of cells and induce cellular apoptosis, and the apoptosis rate of cells is significantly much more obvious in the high group than the low one. This feature show that a time-dose dependence. JQ1 can also reduce the expression of mRNA about FKA and PAK1 with U937 cell, whenever the concentration of it. The result of Western Blot show that the abnormal expression of STAT-5 in the control group was much more higher than that in the experimental group.
Conclusion
JQ1 can effectively inhibit the growth of U937 cells by influenting proliferation and cell apoptosis. Its effect mechanism may be through to influent the expression of FAK and PAK1, thus to influent the STAT - 5 signal pathway (the pathway of cell proliferation).
Session topic: 3. Acute myeloid leukemia - Biology & Translational Research
Keyword(s): Cell line, STAT5, Therapy, Acute monoblastic leukemia