Contributions
Abstract: PB1624
Type: Publication Only
Background
Acute lymphoblastic leukemia (ALL) is recognised as the most common cancers in childhood and a major cause of sickness and death in adults. Recently, CRLF2 rearrangements were frequently identified in Ph-like acute lymphoblastic leukemia with the aggressive disease phenotype.
Aims
In this report, we performed a conventional RT-PCR method to detect P2RY8-CRLF2 fusion which known as the most common CRLF2 rearrangements in Ph-like ALL in childhood.
Methods
Conventional RT-PCR was performed to analyse P2RY8-CRLF2 fusion in RNA isolated from bone marrow samples of 87 newly diagnosed childhood ALL patients. The P2RY8-CRLF2 fusion sequence was subsequently confirmed by using Sanger sequencing technique.
Results
We found that 2 out of 87 patients (2.3%) are positive for P2RY8-CRLF2 fusion. Additionally, one patient with P2RY8-CRLF2 fusion was affected with Down syndrome (trisomy 21). Sequencing analysis on P2RY8-CRLF2 fusion sequences in both two positive samples revealed that the fusion breakpoint is located at 111 nucleotides upper the start codon of P2RY8 sequence (XM_011545631.2) and 2 nucleotides before the start codon of CRLF2 sequence (NM_022148.3).
Conclusion
We could detect a P2RY8-CRLF2 fusion by using a simple RT-PCR technique. Furthermore, P2RY8-CRLF2 fusion sequence was conserved in our tested samples. In recent future, we are going to deeply investigate the function of P2RY8-CRLF2 fusion in the initiation of Ph-like ALL in both in vitro and in mouse model.
Session topic: 1. Acute lymphoblastic leukemia – Biology & Translational Research
Keyword(s): Acute lymphoblastic leukemia, P2Y receptors, Philadelphia chromosome
Abstract: PB1624
Type: Publication Only
Background
Acute lymphoblastic leukemia (ALL) is recognised as the most common cancers in childhood and a major cause of sickness and death in adults. Recently, CRLF2 rearrangements were frequently identified in Ph-like acute lymphoblastic leukemia with the aggressive disease phenotype.
Aims
In this report, we performed a conventional RT-PCR method to detect P2RY8-CRLF2 fusion which known as the most common CRLF2 rearrangements in Ph-like ALL in childhood.
Methods
Conventional RT-PCR was performed to analyse P2RY8-CRLF2 fusion in RNA isolated from bone marrow samples of 87 newly diagnosed childhood ALL patients. The P2RY8-CRLF2 fusion sequence was subsequently confirmed by using Sanger sequencing technique.
Results
We found that 2 out of 87 patients (2.3%) are positive for P2RY8-CRLF2 fusion. Additionally, one patient with P2RY8-CRLF2 fusion was affected with Down syndrome (trisomy 21). Sequencing analysis on P2RY8-CRLF2 fusion sequences in both two positive samples revealed that the fusion breakpoint is located at 111 nucleotides upper the start codon of P2RY8 sequence (XM_011545631.2) and 2 nucleotides before the start codon of CRLF2 sequence (NM_022148.3).
Conclusion
We could detect a P2RY8-CRLF2 fusion by using a simple RT-PCR technique. Furthermore, P2RY8-CRLF2 fusion sequence was conserved in our tested samples. In recent future, we are going to deeply investigate the function of P2RY8-CRLF2 fusion in the initiation of Ph-like ALL in both in vitro and in mouse model.
Session topic: 1. Acute lymphoblastic leukemia – Biology & Translational Research
Keyword(s): Acute lymphoblastic leukemia, P2Y receptors, Philadelphia chromosome