Contributions
Abstract: PB1619
Type: Publication Only
Background
Drug-induced toxicities and dose-related side effects are the major challenges in the conventional cancer therapy by the chemo drugs. On the other hand, herbal derivatives have obtained a great research interest in the field of therapeutic applications because of their more favorable specifications including less toxicity, cost-effective and more physiologically compatible than the chemical drugs. Centaurea genus is one of the current medicinal plants, which has used in traditional medicine, However, there are rare studies to examine its anticancer properties against hematologic malignant cells.
Aims
For this purpose, we evaluated methanolic extract prepared from Centaurea albonitens Turrill extract (CAE) alone and in combination with Vincristine (VCR) for its potential cytotoxic effects in NALM-6, REH, NB4 and KMM-1 cell lines using the various approaches.
Methods
In this experimental study, to explore the effects of CAE and CAE/VCR on hematologic malignant cell lines, the cells were treated with increasing concentrations of extract and drug. Their cytotoxic and anti-proliferative effects were evaluated using trypan blue, MTT assay and DAPI staining. moreover, we performed annexin V/PI staining, cell cycle analysis, Caspase-3 activity assay and real-time RT-PCR to examine the mRNA expression level of BAX, BCL-2, p21 and c-MYC to further investigate how CAE and CAE/VCR exert their cytotoxic properties.
Results
In this study, we demonstrated Centaurea albonitens extract (CAE) induces cytotoxicity through G0/G1 phase arrest followed by apoptosis in a dose- and time- dependent manner, although with varying efficiency. Interestingly, MDBK normal cells didn’t exhibit significant cytotoxicity after CAE treatment. Moreover, we found that low dose of CAE enhances anti-cancer effects of VCR in pre-B ALL cell lines (NALM-6 and REH). Further investigations validated synergistic anticancer activities of VCR and CAE through inducing apoptosis without significant cell cycle arrest.
Conclusion
Taken together, our results demonstrated for the first time that the methanolic extract of Centaurea albonitens can be considered as a potential anticancer agent and/or an enhancer of chemotherapeutic sensitivity of VCR.
Session topic: 1. Acute lymphoblastic leukemia – Biology & Translational Research
Keyword(s): Apoptosis, Hematological malignancy
Abstract: PB1619
Type: Publication Only
Background
Drug-induced toxicities and dose-related side effects are the major challenges in the conventional cancer therapy by the chemo drugs. On the other hand, herbal derivatives have obtained a great research interest in the field of therapeutic applications because of their more favorable specifications including less toxicity, cost-effective and more physiologically compatible than the chemical drugs. Centaurea genus is one of the current medicinal plants, which has used in traditional medicine, However, there are rare studies to examine its anticancer properties against hematologic malignant cells.
Aims
For this purpose, we evaluated methanolic extract prepared from Centaurea albonitens Turrill extract (CAE) alone and in combination with Vincristine (VCR) for its potential cytotoxic effects in NALM-6, REH, NB4 and KMM-1 cell lines using the various approaches.
Methods
In this experimental study, to explore the effects of CAE and CAE/VCR on hematologic malignant cell lines, the cells were treated with increasing concentrations of extract and drug. Their cytotoxic and anti-proliferative effects were evaluated using trypan blue, MTT assay and DAPI staining. moreover, we performed annexin V/PI staining, cell cycle analysis, Caspase-3 activity assay and real-time RT-PCR to examine the mRNA expression level of BAX, BCL-2, p21 and c-MYC to further investigate how CAE and CAE/VCR exert their cytotoxic properties.
Results
In this study, we demonstrated Centaurea albonitens extract (CAE) induces cytotoxicity through G0/G1 phase arrest followed by apoptosis in a dose- and time- dependent manner, although with varying efficiency. Interestingly, MDBK normal cells didn’t exhibit significant cytotoxicity after CAE treatment. Moreover, we found that low dose of CAE enhances anti-cancer effects of VCR in pre-B ALL cell lines (NALM-6 and REH). Further investigations validated synergistic anticancer activities of VCR and CAE through inducing apoptosis without significant cell cycle arrest.
Conclusion
Taken together, our results demonstrated for the first time that the methanolic extract of Centaurea albonitens can be considered as a potential anticancer agent and/or an enhancer of chemotherapeutic sensitivity of VCR.
Session topic: 1. Acute lymphoblastic leukemia – Biology & Translational Research
Keyword(s): Apoptosis, Hematological malignancy