Contributions
Abstract: PB2395
Type: Publication Only
Background
The cobas m 511 system is a novel slide-based automated hematology analyzer that performs a CBC, WBC differential, reticulocyte count, and nucleated RBC count using automated digital microscopy.
Aims
To determine pediatric reference ranges for the 26 parameters measured by the system in a single-center study.
Methods
Residual EDTA whole blood samples were obtained from 245 healthy subjects covering four age ranges (6–≤24 months, 2–≤6 years, 6–≤12 years, and, separately for males and females, 12–≤18 years). A hematologist reviewed the subjects’ medical records to rule out the presence of pathologies or therapies known to affect blood cell counts. Samples were analyzed on the cobas m 511 system and reference ranges were calculated as the central 95% of values obtained for each parameter according to CLSI guideline EP28-A3c.
Results
Reference ranges for each pediatric cohort for the 26 blood count parameters measured by the system are presented in Table 1.
Parameter [units] | 6–≤24 months | 2–≤6 years | 6–≤12 years | 12–≤18 years (females/males) |
WBC [103/µL] | 5.06-14.94 | 4.46-13.42 | 4.28-12.68 | 3.86-11.03 / 3.87-12.53 |
RBC [106/µL] | 3.97-4.98 | 4.01-5.02 | 3.99-4.99 | 3.88-5.18 / 4.09-5.34 |
HGB [g/dL] | 10.8-13.3 | 11.0-13.6 | 11.6-14.1 | 11.1-15.0 / 12.3-15.9 |
HCT [%] | 31.7-38.3 | 32.7-40.1 | 33.5-42.1 | 33.4-44.0 / 36.6-47.7 |
MCV [fL] | 72.4-86.0 | 72.5-87.5 | 71.6-94.6 | 71.9-95.4 / 79.7-96.5 |
MCH [pg] | 23.9-29.3 | 23.2-29.9 | 24.0-32.0 | 24.8-31.9 / 26.5-31.7 |
MCHC [g/dL] | 32.4-35.1 | 32.4-34.9 | 32.4-35.4 | 32.2-35.1 / 32.5-34.5 |
RDW [%] | 12.3-16.3 | 12.8-16.3 | 12.3-14.7 | 12.1-16.7 / 12.1-14.3 |
RDW-SD [fL] | 35.3-46.0 | 36.6-45.8 | 35.2-44.7 | 37.0-47.2 / 37.4-45.6 |
PLT [103/µL] | 222-551 | 213-579 | 199-420 | 184-409 / 138-409 |
MPV [fL] | 7.4-10.3 | 7.4-10.0 | 8.0-11.6 | 8.3-11.9 / 8.2-12.9 |
#NRBC [103/µL] | 0.00-0.01 | 0.00-0.01 | 0.00-0.02 | 0.00-0.01 / 0.00-0.03 |
#NEUT [103/µL] | 1.45-5.64 | 1.18-6.51 | 1.63-6.90 | 1.69-6.70 / 1.50-9.55 |
#LYMPH [103/µL] | 2.68-9.85 | 2.27-6.86 | 1.20-5.82 | 1.40-3.40 / 1.27-3.24 |
#MONO [103/µL] | 0.38-1.51 | 0.34-1.07 | 0.29-0.91 | 0.22-1.09 / 0.35-1.30 |
#EO [103/µL] | 0.02-0.91 | 0.07-1.82 | 0.01-1.37 | 0.01-0.51 / 0.02-0.64 |
#BASO [103/µL] | 0.00-0.16 | 0.00-0.12 | 0.00-0.13 | 0.00-0.12 / 0.01-0.12 |
#RET [106/µL] | 0.02-0.06 | 0.01-0.06 | 0.02-0.08 | 0.02-0.06 / 0.01-0.08 |
HGB-RET [pg] | 24.1-34.3 | 27.1-35.3 | 27.1-36.1 | 28.4-36.8 / 29.4-37.9 |
Data for %NRBC, %NEUT, %LYMPH, %MONO, %EO, %BASO, and %RET not shown | ||||
Conclusion
The observed reference range for the 26 parameters analyzed on the cobas m 511 Hematology Analyzer, and the age and sex differences, are consistent with reference ranges determined for other automated hematology analyzers.
Session topic: 36. Quality of life, palliative care, ethics and health economics
Keyword(s): Cytometry, Pediatric
Abstract: PB2395
Type: Publication Only
Background
The cobas m 511 system is a novel slide-based automated hematology analyzer that performs a CBC, WBC differential, reticulocyte count, and nucleated RBC count using automated digital microscopy.
Aims
To determine pediatric reference ranges for the 26 parameters measured by the system in a single-center study.
Methods
Residual EDTA whole blood samples were obtained from 245 healthy subjects covering four age ranges (6–≤24 months, 2–≤6 years, 6–≤12 years, and, separately for males and females, 12–≤18 years). A hematologist reviewed the subjects’ medical records to rule out the presence of pathologies or therapies known to affect blood cell counts. Samples were analyzed on the cobas m 511 system and reference ranges were calculated as the central 95% of values obtained for each parameter according to CLSI guideline EP28-A3c.
Results
Reference ranges for each pediatric cohort for the 26 blood count parameters measured by the system are presented in Table 1.
Parameter [units] | 6–≤24 months | 2–≤6 years | 6–≤12 years | 12–≤18 years (females/males) |
WBC [103/µL] | 5.06-14.94 | 4.46-13.42 | 4.28-12.68 | 3.86-11.03 / 3.87-12.53 |
RBC [106/µL] | 3.97-4.98 | 4.01-5.02 | 3.99-4.99 | 3.88-5.18 / 4.09-5.34 |
HGB [g/dL] | 10.8-13.3 | 11.0-13.6 | 11.6-14.1 | 11.1-15.0 / 12.3-15.9 |
HCT [%] | 31.7-38.3 | 32.7-40.1 | 33.5-42.1 | 33.4-44.0 / 36.6-47.7 |
MCV [fL] | 72.4-86.0 | 72.5-87.5 | 71.6-94.6 | 71.9-95.4 / 79.7-96.5 |
MCH [pg] | 23.9-29.3 | 23.2-29.9 | 24.0-32.0 | 24.8-31.9 / 26.5-31.7 |
MCHC [g/dL] | 32.4-35.1 | 32.4-34.9 | 32.4-35.4 | 32.2-35.1 / 32.5-34.5 |
RDW [%] | 12.3-16.3 | 12.8-16.3 | 12.3-14.7 | 12.1-16.7 / 12.1-14.3 |
RDW-SD [fL] | 35.3-46.0 | 36.6-45.8 | 35.2-44.7 | 37.0-47.2 / 37.4-45.6 |
PLT [103/µL] | 222-551 | 213-579 | 199-420 | 184-409 / 138-409 |
MPV [fL] | 7.4-10.3 | 7.4-10.0 | 8.0-11.6 | 8.3-11.9 / 8.2-12.9 |
#NRBC [103/µL] | 0.00-0.01 | 0.00-0.01 | 0.00-0.02 | 0.00-0.01 / 0.00-0.03 |
#NEUT [103/µL] | 1.45-5.64 | 1.18-6.51 | 1.63-6.90 | 1.69-6.70 / 1.50-9.55 |
#LYMPH [103/µL] | 2.68-9.85 | 2.27-6.86 | 1.20-5.82 | 1.40-3.40 / 1.27-3.24 |
#MONO [103/µL] | 0.38-1.51 | 0.34-1.07 | 0.29-0.91 | 0.22-1.09 / 0.35-1.30 |
#EO [103/µL] | 0.02-0.91 | 0.07-1.82 | 0.01-1.37 | 0.01-0.51 / 0.02-0.64 |
#BASO [103/µL] | 0.00-0.16 | 0.00-0.12 | 0.00-0.13 | 0.00-0.12 / 0.01-0.12 |
#RET [106/µL] | 0.02-0.06 | 0.01-0.06 | 0.02-0.08 | 0.02-0.06 / 0.01-0.08 |
HGB-RET [pg] | 24.1-34.3 | 27.1-35.3 | 27.1-36.1 | 28.4-36.8 / 29.4-37.9 |
Data for %NRBC, %NEUT, %LYMPH, %MONO, %EO, %BASO, and %RET not shown | ||||
Conclusion
The observed reference range for the 26 parameters analyzed on the cobas m 511 Hematology Analyzer, and the age and sex differences, are consistent with reference ranges determined for other automated hematology analyzers.
Session topic: 36. Quality of life, palliative care, ethics and health economics
Keyword(s): Cytometry, Pediatric