Contributions
Abstract: PB2040
Type: Publication Only
Background
In the new WHO 2016 classification of lymphomas, the relative importance of each of the morphological, immunophenotypic, genetic and clinical criteria varies from one lymphoma to another, but the morphological and immunophenotypic criteria remain the cornerstone of the diagnosis. As a result, cytology and CMF coupling by fine needle aspiration puncture are easy and valuable tools for the diagnosis and follow-up of lymphoproliferative disorders, especially small-cell NHL-B-cells.
Aims
To demonstrate the value of fine needle aspiration coupled with CMF for rapid outpatient diagnosis and follow-up of small-cell NHL B.
Methods
Retrospective and descriptive study over a period of 6 years (from January 2010 to December 2015). We collected 180 samples including 134 evaluable.
The diagnosis of small cell lymphoma was made in 38 patients (28%), 33 lymphadenopathies (by fine needle aspiration (23 G)), 3 ascites fluid, 1 pleural fluid and 1 subcutaneous mass. skin.
A cytological study (MGG) was performed on the various samples.
Labeling with more than 10 Antibodies on average for each sample. Then we proceed to the acquisition and analysis by flow cytometry (FACS CALIBUR 3 colors of Becton Dickinson).
The histological study with immunohistochemistry was performed in 33 patients: 21 biopsies of adenopathy (14 biopsies of ADP alone, 6 with PBO, 1 cutaneous biopsy), 2 PBO alone, 1 tonsillectomy, 1 transparietal biopsy associated with a PBO, 1 exeresis biopsy of a hepatic mass and 1 gastrectomy. The 5 unexplained biopsies for the following reasons: 3 cases of ascites whose biopsy is difficult and 2 cases of relapse.
Results
In our series, the average age of patients was 61 years (22-83 years), with a clear male predominance (26 men and 12 women, sex ratio 2.5).
At the CMF, several diagnoses and types of NHL could be made: follicular NHL 10 cases (26%), NHL with small cell without being able to specify the exact type 9 cases (24%), lymphocytic NHL 8 cases (21%), NHL to coat cells 7 cases (18%), NHL marginal area 2 cases (5%), lymphoplasmocytic NHL 2 cases (5%).
In the histology, the diagnostics performed in 33 patients are as follows: 3 cases of LGCDB (41%), small cell NHL 5 cases (8%), follicular NHL 8 cases (7%), NHL with mantle cells 6 case (5%), marginal zone NHL 1 case (5%), lymphocytic NHL 7 cases (2%), angioimmunoblastic NHL 1 case (4%), inflammatory (reactive) 2 cases (5%).
After these results, the overall correlation between CMF and histology is 94% in the diagnosis of NHL B: 70% the CMF allowed to make the diagnosis and to type the NHL, in 24% one finds a concordance in the diagnosis without being able to specify the morphological type and 6% of discordance (2 false positives in CMF).
Conclusion
when the fine needle aspiration coupled with CMF is performed by experienced operators and for the correct indications, it is one of the safest technique of small cell NHL B diagnostic and less massive, more profitable and more accurate.
Session topic: 20. Indolent Non-Hodgkin lymphoma – Clinical
Keyword(s): flow cytometry, lymphoma, Lymphoproliferative disorder
Abstract: PB2040
Type: Publication Only
Background
In the new WHO 2016 classification of lymphomas, the relative importance of each of the morphological, immunophenotypic, genetic and clinical criteria varies from one lymphoma to another, but the morphological and immunophenotypic criteria remain the cornerstone of the diagnosis. As a result, cytology and CMF coupling by fine needle aspiration puncture are easy and valuable tools for the diagnosis and follow-up of lymphoproliferative disorders, especially small-cell NHL-B-cells.
Aims
To demonstrate the value of fine needle aspiration coupled with CMF for rapid outpatient diagnosis and follow-up of small-cell NHL B.
Methods
Retrospective and descriptive study over a period of 6 years (from January 2010 to December 2015). We collected 180 samples including 134 evaluable.
The diagnosis of small cell lymphoma was made in 38 patients (28%), 33 lymphadenopathies (by fine needle aspiration (23 G)), 3 ascites fluid, 1 pleural fluid and 1 subcutaneous mass. skin.
A cytological study (MGG) was performed on the various samples.
Labeling with more than 10 Antibodies on average for each sample. Then we proceed to the acquisition and analysis by flow cytometry (FACS CALIBUR 3 colors of Becton Dickinson).
The histological study with immunohistochemistry was performed in 33 patients: 21 biopsies of adenopathy (14 biopsies of ADP alone, 6 with PBO, 1 cutaneous biopsy), 2 PBO alone, 1 tonsillectomy, 1 transparietal biopsy associated with a PBO, 1 exeresis biopsy of a hepatic mass and 1 gastrectomy. The 5 unexplained biopsies for the following reasons: 3 cases of ascites whose biopsy is difficult and 2 cases of relapse.
Results
In our series, the average age of patients was 61 years (22-83 years), with a clear male predominance (26 men and 12 women, sex ratio 2.5).
At the CMF, several diagnoses and types of NHL could be made: follicular NHL 10 cases (26%), NHL with small cell without being able to specify the exact type 9 cases (24%), lymphocytic NHL 8 cases (21%), NHL to coat cells 7 cases (18%), NHL marginal area 2 cases (5%), lymphoplasmocytic NHL 2 cases (5%).
In the histology, the diagnostics performed in 33 patients are as follows: 3 cases of LGCDB (41%), small cell NHL 5 cases (8%), follicular NHL 8 cases (7%), NHL with mantle cells 6 case (5%), marginal zone NHL 1 case (5%), lymphocytic NHL 7 cases (2%), angioimmunoblastic NHL 1 case (4%), inflammatory (reactive) 2 cases (5%).
After these results, the overall correlation between CMF and histology is 94% in the diagnosis of NHL B: 70% the CMF allowed to make the diagnosis and to type the NHL, in 24% one finds a concordance in the diagnosis without being able to specify the morphological type and 6% of discordance (2 false positives in CMF).
Conclusion
when the fine needle aspiration coupled with CMF is performed by experienced operators and for the correct indications, it is one of the safest technique of small cell NHL B diagnostic and less massive, more profitable and more accurate.
Session topic: 20. Indolent Non-Hodgkin lymphoma – Clinical
Keyword(s): flow cytometry, lymphoma, Lymphoproliferative disorder