Contributions
Abstract: PB1882
Type: Publication Only
Background
The cobas m 511 integrated hematology analyzer (cobas m 511 system) is a novel slide-based system that performs a CBC, WBC differential, reticulocyte count, and nucleated RBC count using digital analysis.
Aims
This single-center study investigated whether the cobas m 511 system delivered comparable results to the Sysmex® XN-10 Automated Hematology Analyzer using samples from patients with medical conditions.
Methods
Laboratory hematology results were reviewed to identify subjects with 23 targeted medical conditions (including hematological malignancies and disorders of cell numbers and function). Residual whole blood samples (n=130) were processed on both systems within 8 hours of venipuncture. Consistent with CLSI EP09-A3, a method comparison was used to assess the correlation and bias between the systems for all parameters. Individual patient parameter results that were valid on both instruments were included.
Results
All 26 reportable parameters evaluated showed good-to-excellent correlation between the automated results of the cobas m 511 system and Sysmex Analyzer, with no significant bias (Table 1).
Parameter [units] | Sample range | Pearson’s R | Intercept | Slope |
WBC [103/µL] | (0.11-95.41) | 0.999 | -0.02 | 0.995 |
RBC [106/µL] | (1.79-7.68) | 0.996 | -0.01 | 0.992 |
HGB [g/dL] | (6.28-17.24) | 0.995 | -0.31 | 1.064 |
HCT [%] | (18.60-56.00) | 0.982 | -0.53 | 1.034 |
MCV [fL] | (69.50-107.80) | 0.879 | 5.35 | 0.975 |
MCH [pg] | (20.22-36.48) | 0.977 | 2.87 | 0.946 |
MCHC [g/dL] | (28.20-36.42) | 0.548 | 14.88 | 0.559 |
RDW [%] | (11.50-27.10) | 0.929 | 2.81 | 0.850 |
RDW-SD [fL] | (34.10-93.00) | 0.908 | 7.46 | 0.910 |
PLT [103/µL] | (9.00-1379.00) | 0.994 | -2.02 | 0.943 |
MPV [fL] | (8.40-13.00) | 0.843 | 0.82 | 0.915 |
#NRBC [103/µL] | (0.00-4.44) | 0.980 | N/A | N/A |
#NEUT [103/µL] | (0.46-36.82) | 0.999 | 0.03 | 1.008 |
#LYMPH [103/µL] | (0.16-4.89) | 0.985 | 0.01 | 0.976 |
#MONO [103/µL] | (0.14-7.65) | 0.991 | -0.03 | 1.013 |
#EO [103/µL] | (0.00-1.23) | 0.978 | 0.00 | 1.031 |
#BASO [103/µL] | (0.00-2.51) | 0.962 | -0.08 | 1.829 |
#RET [106/µL] | (0.00-0.34) | 0.971 | -0.01 | 0.982 |
HGB-RET [pg] | (14.68-42.04) | 0.934 | -2.75 | 1.193 |
Data for %NRBC, %NEUT, %LYMPH, %MONO, %EO, %BASO, and %RET not shown | ||||
Conclusion
The cobas m 511 system and Sysmex Analyzer produce comparable results for samples with targeted medical conditions. This demonstrates the robustness of the cobas m 511 system when abnormal samples are encountered.
Session topic: 6. Chronic lymphocytic leukemia and related disorders - Clinical
Keyword(s): Cytometry, flow cytometry
Abstract: PB1882
Type: Publication Only
Background
The cobas m 511 integrated hematology analyzer (cobas m 511 system) is a novel slide-based system that performs a CBC, WBC differential, reticulocyte count, and nucleated RBC count using digital analysis.
Aims
This single-center study investigated whether the cobas m 511 system delivered comparable results to the Sysmex® XN-10 Automated Hematology Analyzer using samples from patients with medical conditions.
Methods
Laboratory hematology results were reviewed to identify subjects with 23 targeted medical conditions (including hematological malignancies and disorders of cell numbers and function). Residual whole blood samples (n=130) were processed on both systems within 8 hours of venipuncture. Consistent with CLSI EP09-A3, a method comparison was used to assess the correlation and bias between the systems for all parameters. Individual patient parameter results that were valid on both instruments were included.
Results
All 26 reportable parameters evaluated showed good-to-excellent correlation between the automated results of the cobas m 511 system and Sysmex Analyzer, with no significant bias (Table 1).
Parameter [units] | Sample range | Pearson’s R | Intercept | Slope |
WBC [103/µL] | (0.11-95.41) | 0.999 | -0.02 | 0.995 |
RBC [106/µL] | (1.79-7.68) | 0.996 | -0.01 | 0.992 |
HGB [g/dL] | (6.28-17.24) | 0.995 | -0.31 | 1.064 |
HCT [%] | (18.60-56.00) | 0.982 | -0.53 | 1.034 |
MCV [fL] | (69.50-107.80) | 0.879 | 5.35 | 0.975 |
MCH [pg] | (20.22-36.48) | 0.977 | 2.87 | 0.946 |
MCHC [g/dL] | (28.20-36.42) | 0.548 | 14.88 | 0.559 |
RDW [%] | (11.50-27.10) | 0.929 | 2.81 | 0.850 |
RDW-SD [fL] | (34.10-93.00) | 0.908 | 7.46 | 0.910 |
PLT [103/µL] | (9.00-1379.00) | 0.994 | -2.02 | 0.943 |
MPV [fL] | (8.40-13.00) | 0.843 | 0.82 | 0.915 |
#NRBC [103/µL] | (0.00-4.44) | 0.980 | N/A | N/A |
#NEUT [103/µL] | (0.46-36.82) | 0.999 | 0.03 | 1.008 |
#LYMPH [103/µL] | (0.16-4.89) | 0.985 | 0.01 | 0.976 |
#MONO [103/µL] | (0.14-7.65) | 0.991 | -0.03 | 1.013 |
#EO [103/µL] | (0.00-1.23) | 0.978 | 0.00 | 1.031 |
#BASO [103/µL] | (0.00-2.51) | 0.962 | -0.08 | 1.829 |
#RET [106/µL] | (0.00-0.34) | 0.971 | -0.01 | 0.982 |
HGB-RET [pg] | (14.68-42.04) | 0.934 | -2.75 | 1.193 |
Data for %NRBC, %NEUT, %LYMPH, %MONO, %EO, %BASO, and %RET not shown | ||||
Conclusion
The cobas m 511 system and Sysmex Analyzer produce comparable results for samples with targeted medical conditions. This demonstrates the robustness of the cobas m 511 system when abnormal samples are encountered.
Session topic: 6. Chronic lymphocytic leukemia and related disorders - Clinical
Keyword(s): Cytometry, flow cytometry