Contributions
Abstract: PB2225
Type: Publication Only
Background
Many plants have an effect on the blood clotting system. It is known that there are heparin-like substances in some types of peony roots (Paeonia lactiflora, Paeonia suffruticosa). It proved that there is an anticoagulant activity in extracts from such roots.
Aims
The intention is to show the inhibitory effect of the extract of Paeonia lactiflora roots (EA) on processes fibrin and thrombus formation.
Methods
We used the standard coagulologic methods for determining anticoagulant activity by APTT test, antiplatelet, total fibrinolytic activity (TFA), fibrindepolymerizing activity (FDPA). Experiments were carried out in accordance with ethical principles and documents recommended by the Declaration of Helsinki of the humane treatment of animals. We used an animal model with prothrombotic condition caused by intravenous administration of subthreshold doses of tissue thromboplastin at a dose of 0.6 -0.7 ml per 200 g body weight in rats. After 30 min after injection of thromboplastin, we injected intraperitoneally 0.1 mL of 1% of extract of EP and after 30 minutes we determined parameters of hemostasis in the blood plasma.
Results
It was shown that after administration of the indicated doses thromboplastin occurs hypercoagulability in blood plasma of animals (APTT decreased by 23% SFA - 15%, FDPA -12%; increased platelet aggregation by 18% compared to control animals not receiving thromboplastin). Normalization of blood clotting is installed in the experimental rats after application EP (recovery of platelet aggregation to 98%, APTT- to 100%, up to 95% SFA- FDPA and - up to 67% compared with control). The high degree of FDPA indicates the ability of EP to obstruct the process of the formation of fibrins and thrombosis. Heparin components in EP interact with fibrin monomers which do not participate in their conversion to fibrin polymer. As a result, stable fibrin polymer or thrombus is not formed.
Conclusion
Consequently, the extract of Paeonia lactiflora roots containing heparinoid contributes to the restoration of coagulation properties in blood of animals in prothrombotic condition and prevents thrombosis. In the initial stages of fibrin formation, it causes the thrombus dissolution.
Session topic: 34. Thrombosis and vascular biology
Keyword(s): Thrombosis, Fibrinolysis, Endothelial, Anticoagulants
Abstract: PB2225
Type: Publication Only
Background
Many plants have an effect on the blood clotting system. It is known that there are heparin-like substances in some types of peony roots (Paeonia lactiflora, Paeonia suffruticosa). It proved that there is an anticoagulant activity in extracts from such roots.
Aims
The intention is to show the inhibitory effect of the extract of Paeonia lactiflora roots (EA) on processes fibrin and thrombus formation.
Methods
We used the standard coagulologic methods for determining anticoagulant activity by APTT test, antiplatelet, total fibrinolytic activity (TFA), fibrindepolymerizing activity (FDPA). Experiments were carried out in accordance with ethical principles and documents recommended by the Declaration of Helsinki of the humane treatment of animals. We used an animal model with prothrombotic condition caused by intravenous administration of subthreshold doses of tissue thromboplastin at a dose of 0.6 -0.7 ml per 200 g body weight in rats. After 30 min after injection of thromboplastin, we injected intraperitoneally 0.1 mL of 1% of extract of EP and after 30 minutes we determined parameters of hemostasis in the blood plasma.
Results
It was shown that after administration of the indicated doses thromboplastin occurs hypercoagulability in blood plasma of animals (APTT decreased by 23% SFA - 15%, FDPA -12%; increased platelet aggregation by 18% compared to control animals not receiving thromboplastin). Normalization of blood clotting is installed in the experimental rats after application EP (recovery of platelet aggregation to 98%, APTT- to 100%, up to 95% SFA- FDPA and - up to 67% compared with control). The high degree of FDPA indicates the ability of EP to obstruct the process of the formation of fibrins and thrombosis. Heparin components in EP interact with fibrin monomers which do not participate in their conversion to fibrin polymer. As a result, stable fibrin polymer or thrombus is not formed.
Conclusion
Consequently, the extract of Paeonia lactiflora roots containing heparinoid contributes to the restoration of coagulation properties in blood of animals in prothrombotic condition and prevents thrombosis. In the initial stages of fibrin formation, it causes the thrombus dissolution.
Session topic: 34. Thrombosis and vascular biology
Keyword(s): Thrombosis, Fibrinolysis, Endothelial, Anticoagulants