Contributions
Abstract: PB2221
Type: Publication Only
Background
A lot of bio-chemical compounds from secretion of the amphibian skin glands with various biological activities have been isolated and characterized. Several recent studies indicate that amphibian skin secretions can be a source of molecules affecting the platelet activity. We are interested to look for other bioactive components of the amphibian skin which exhibit ability to influence on diverse parameters of hemostatic system.
Aims
Methods
Results
The lyophilized B. bufo skin secretions in dose-dependent manner induced platelet aggregation in both PRP and purified PF; its final concentration of 50 mg of total protein/mL caused the same effect as 5x10-6M ADP. These results indicated that skin components acted directly on platelets, maybe through their surface receptors. The lyophilized skin secretions of B. variegata and B. viridis also activated platelet aggregation but their effects were lower than B. bufo skin secretions. The skin secretions from all studied amphibian did not influence on PT and TT except B. viridis which prolonged TT by 40%. The values of APTT were significantly enhanced in 3.4 and 2.3 times under the influence of crude skin secretions (final concentration of 0.2 mg total protein/mL plasma) of B. bombina and B. variegata, respectively.
Conclusion
The obtained results indicate the prospects of the search for potential modulators of hemostatic system among the amphibian skin bioactive compounds. To establish their physiological and functional mechanisms of action, the further purification and characterization of components from the skin gland secretions are necessary.
Session topic: 34. Thrombosis and vascular biology
Keyword(s): Treatment, Platelet aggregation, Coagulation, Anticoagulants
Abstract: PB2221
Type: Publication Only
Background
A lot of bio-chemical compounds from secretion of the amphibian skin glands with various biological activities have been isolated and characterized. Several recent studies indicate that amphibian skin secretions can be a source of molecules affecting the platelet activity. We are interested to look for other bioactive components of the amphibian skin which exhibit ability to influence on diverse parameters of hemostatic system.
Aims
Methods
Results
The lyophilized B. bufo skin secretions in dose-dependent manner induced platelet aggregation in both PRP and purified PF; its final concentration of 50 mg of total protein/mL caused the same effect as 5x10-6M ADP. These results indicated that skin components acted directly on platelets, maybe through their surface receptors. The lyophilized skin secretions of B. variegata and B. viridis also activated platelet aggregation but their effects were lower than B. bufo skin secretions. The skin secretions from all studied amphibian did not influence on PT and TT except B. viridis which prolonged TT by 40%. The values of APTT were significantly enhanced in 3.4 and 2.3 times under the influence of crude skin secretions (final concentration of 0.2 mg total protein/mL plasma) of B. bombina and B. variegata, respectively.
Conclusion
The obtained results indicate the prospects of the search for potential modulators of hemostatic system among the amphibian skin bioactive compounds. To establish their physiological and functional mechanisms of action, the further purification and characterization of components from the skin gland secretions are necessary.
Session topic: 34. Thrombosis and vascular biology
Keyword(s): Treatment, Platelet aggregation, Coagulation, Anticoagulants