Contributions
Abstract: PB2214
Type: Publication Only
Background
Thrombosis is often multifactorial, caused by both genetic and acquired risk factors. The inflammatory process is linked to pathogenesis of venous thrombosis. Venous thrombosis is considered to be mediated by an imbalance in proinflammatory as compared with anti-inflammatory molecules. One of the important anti-inflammatory cytokine is interleukin-10 (IL-10) with important immunoregulatory functions. Primarily, IL-10 counterbalances the potentially harmful effects of tumor necrosis factor α (TNFα) and other pro-inflammatory mediator such as IL-1b, IL-6, and IL-8 from monocytes/macrophages. Three important single nucleotide polymorphisms (SNP) affect IL-10 expression, including: 1082 A/G, 819 C/T, and 592 C/A. Studying the association between genetic polymorphisms of anti-inflammatory cytokines such as IL-10, and venous thrombosis may suggest using of polymorphisms as a predictive genetic marker of future VTE.
Aims
The objective of this study was to evaluate a possible association between IL-10 -1082A/G, and -592C/A polymorphisms with DVT.
Methods
The study was conducted on 115 patients with symptomatic DVT proved by venous duplex ultrasound; divided into two cohorts: group A included 60 patients with unprovoked DVT, and group B included 55 patients with provoked DVT. Gene mutations for IL-10 -1082A/G, and -592C/A were performed using PCR-restriction fragment length polymorphism assay. We studied the association between IL-10 gene polymorphisms and occurrence of either provoked or non-provoked DVT. We also investigated the link between these polymorphisms and the recurrence of DVT and family history of DVT.
Results
in our study IL101082AG gene analysis revealed that mutant genotypes distribution is statistically significant different compared to the wild genotype distribution, being higher in group A (with unprovoked DVT) than in group B (with provoked DVT); as GG genotype was detected in 14 patients (63.6%) versus 8 patients (36.4%) in group A and B respectively (P value = 0.037); AG genotype was detected in 30 patients (63.8%) compared to 17 patients (36.2%) in group A and B respectively (P value = 0.007). However, there is no correlation was found between IL101082 mutant genotypes distribution and VTE recurrence (P value= 0.738 and 1 respectively) or positive family history of VTE (P value= 0.101 and 0.714 respectively), compared to wild genotype.
Conclusion
I IL101082AG gene polymorphism is associated with risk of unprovoked DVT, however it is not associated with either risk of recurrence or positive family history.
Session topic: 34. Thrombosis and vascular biology
Keyword(s): Gene polymorphism, DVT, IL-10
Abstract: PB2214
Type: Publication Only
Background
Thrombosis is often multifactorial, caused by both genetic and acquired risk factors. The inflammatory process is linked to pathogenesis of venous thrombosis. Venous thrombosis is considered to be mediated by an imbalance in proinflammatory as compared with anti-inflammatory molecules. One of the important anti-inflammatory cytokine is interleukin-10 (IL-10) with important immunoregulatory functions. Primarily, IL-10 counterbalances the potentially harmful effects of tumor necrosis factor α (TNFα) and other pro-inflammatory mediator such as IL-1b, IL-6, and IL-8 from monocytes/macrophages. Three important single nucleotide polymorphisms (SNP) affect IL-10 expression, including: 1082 A/G, 819 C/T, and 592 C/A. Studying the association between genetic polymorphisms of anti-inflammatory cytokines such as IL-10, and venous thrombosis may suggest using of polymorphisms as a predictive genetic marker of future VTE.
Aims
The objective of this study was to evaluate a possible association between IL-10 -1082A/G, and -592C/A polymorphisms with DVT.
Methods
The study was conducted on 115 patients with symptomatic DVT proved by venous duplex ultrasound; divided into two cohorts: group A included 60 patients with unprovoked DVT, and group B included 55 patients with provoked DVT. Gene mutations for IL-10 -1082A/G, and -592C/A were performed using PCR-restriction fragment length polymorphism assay. We studied the association between IL-10 gene polymorphisms and occurrence of either provoked or non-provoked DVT. We also investigated the link between these polymorphisms and the recurrence of DVT and family history of DVT.
Results
in our study IL101082AG gene analysis revealed that mutant genotypes distribution is statistically significant different compared to the wild genotype distribution, being higher in group A (with unprovoked DVT) than in group B (with provoked DVT); as GG genotype was detected in 14 patients (63.6%) versus 8 patients (36.4%) in group A and B respectively (P value = 0.037); AG genotype was detected in 30 patients (63.8%) compared to 17 patients (36.2%) in group A and B respectively (P value = 0.007). However, there is no correlation was found between IL101082 mutant genotypes distribution and VTE recurrence (P value= 0.738 and 1 respectively) or positive family history of VTE (P value= 0.101 and 0.714 respectively), compared to wild genotype.
Conclusion
I IL101082AG gene polymorphism is associated with risk of unprovoked DVT, however it is not associated with either risk of recurrence or positive family history.
Session topic: 34. Thrombosis and vascular biology
Keyword(s): Gene polymorphism, DVT, IL-10