EHA Library - The official digital education library of European Hematology Association (EHA)

RED BLOOD CELL EXTENDED PARAMETERS IN HAEMOGLOBINOPATHIES
Author(s):
Maria Stamou
,
Maria Stamou
Affiliations:
Laboratory Haematology,General Hospital of Xanthi,Xanthi,Greece
Eleni Petridou
,
Eleni Petridou
Affiliations:
Laboratory Haematology,General Hospital of Xanthi,Xanthi,Greece
Agapi Kotanidou
,
Agapi Kotanidou
Affiliations:
Laboratory Haematology,General Hospital of Xanthi,Xanthi,Greece
Athanasia Agorasti
Athanasia Agorasti
Affiliations:
Laboratory Haematology,General Hospital of Xanthi,Xanthi,Greece
(Abstract release date: 05/18/17) EHA Library. Agorasti A. 05/18/17; 182910; PB2197
Dr. Athanasia Agorasti
Dr. Athanasia Agorasti
Contributions
PDF Abstract
Abstract

Abstract: PB2197

Type: Publication Only

Background

Sysmex® XE-5000 analyzer incorporates new research Red Blood Cell (RBC) parameters, derived from flow fluorescence cytometry technology, including %HYPO-He, which indicates the percentage of RBC with haemoglobin (Hb) content < 17 pg, and %MicroR which indicates the percentage of RBC with mean cell volume < 60 fL.

Aims
The aim of this study was to establish the reference range of our Laboratory for the parameters %HYPO-He & %MicroR, to investigate their values in haemoglobinopathies and their correlation, if any, with Hb A2 levels in heterozygous β-thalassaemia.

Methods

Reference ranges were obtained from 175 healthy adult subjects (27 men, median age of 34 years & 148 women, median age of 30 years); control group (group A). 89 haemoglobinopathie heterozygotes (32 men, median age of 29 years & 57 women, median age of 30 years) were included in the study and classified into three groups; group B: β-thalassaemia heterozygotes, N = 46, group C: α-thalassaemia heterozygotes, N = 21 and group D: Hb O-Arab heterozygotes, N = 22. We retrospectively recorded the results of full blood count analysis on Sysmex® XE-5000 analyzer including %HYPO-He & %MicroR, of Hb pattern analysis (TOSOH®, G7) and ferritin levels (Roche®, cobas e411). All subjects included in the study presented ferritin levels within the normal range for age and gender. Statistical analysis: one-way ANOVA (Tukey post-hoc), Mann-Whitney, Pearson’s correlation tests were applied. Reference ranges were calculated as the mean ± 2SD of the distribution. P value < 0.05 was considered to be statistically significant. Data refer as median (percentiles).

Results
The reference ranges of our Laboratory for the parameters %HYPO-He & %MicroR are 0.0 – 0.6% & 0.2 – 2.9%, respectively, and they are independent of gender and age (P=0.715, P=0.168 & P=0.073, P=0.843). There was a statistically significant difference between the groups as determined by one-way ANOVA for both parameters (all P < 0.0001). Heterozygous β-thalassaemia presents statistically significantly higher %HYPO-He values [11.6 (4.2-27.6)] as compared to groups A [0.3 (0.2-0.3)], C [1.9 (0.6-4.6)], D [0.6 (0.4-0.8)] (all P < 0.0001), while there was no statistically significant difference of %HYPO-He values between heterozygous Hb O-Arab and groups A and C (P = 0.965 & P = 0.134, respectively) based on Tukey post hoc test. Heterozygous β-thalassaemia presents statistically significantly higher %MicroR values [41.5 (22.9-58.7)] as compared to groups A [1.5 (1.1-2.0)], C [10.8 (7.9-20.5)] and D [6.1 (4.2-9.0)] (all P < 0.0001). Both parameters %HYPO-He & %MicroR are correlated positively in a statistically significant degree with Hb A2 levels in heterozygous β-thalassaemia (r = 0.567, P < 0.0001, y = 0.050x + 5.317 & r = 0.680, P < 0.0001, y = 0.042x + 4.371, respectively).

Conclusion
β-thalassaemia heterozygotes present higher values of %HYPO-He & %MicroR compared to α-thalassaemia and Hb O-Arab heterozygotes. %HYPO-He & %MicroR are possibly useful parameters for evaluating the relative severity of different genotypes in heterozygous β-thalassaemia because of the statistically significant positive correlation with Hb A2 levels.

Session topic: 26. Thalassemias

Keyword(s): Red blood cell, Hemoglobinopathy, Anemia

Abstract: PB2197

Type: Publication Only

Background

Sysmex® XE-5000 analyzer incorporates new research Red Blood Cell (RBC) parameters, derived from flow fluorescence cytometry technology, including %HYPO-He, which indicates the percentage of RBC with haemoglobin (Hb) content < 17 pg, and %MicroR which indicates the percentage of RBC with mean cell volume < 60 fL.

Aims
The aim of this study was to establish the reference range of our Laboratory for the parameters %HYPO-He & %MicroR, to investigate their values in haemoglobinopathies and their correlation, if any, with Hb A2 levels in heterozygous β-thalassaemia.

Methods

Reference ranges were obtained from 175 healthy adult subjects (27 men, median age of 34 years & 148 women, median age of 30 years); control group (group A). 89 haemoglobinopathie heterozygotes (32 men, median age of 29 years & 57 women, median age of 30 years) were included in the study and classified into three groups; group B: β-thalassaemia heterozygotes, N = 46, group C: α-thalassaemia heterozygotes, N = 21 and group D: Hb O-Arab heterozygotes, N = 22. We retrospectively recorded the results of full blood count analysis on Sysmex® XE-5000 analyzer including %HYPO-He & %MicroR, of Hb pattern analysis (TOSOH®, G7) and ferritin levels (Roche®, cobas e411). All subjects included in the study presented ferritin levels within the normal range for age and gender. Statistical analysis: one-way ANOVA (Tukey post-hoc), Mann-Whitney, Pearson’s correlation tests were applied. Reference ranges were calculated as the mean ± 2SD of the distribution. P value < 0.05 was considered to be statistically significant. Data refer as median (percentiles).

Results
The reference ranges of our Laboratory for the parameters %HYPO-He & %MicroR are 0.0 – 0.6% & 0.2 – 2.9%, respectively, and they are independent of gender and age (P=0.715, P=0.168 & P=0.073, P=0.843). There was a statistically significant difference between the groups as determined by one-way ANOVA for both parameters (all P < 0.0001). Heterozygous β-thalassaemia presents statistically significantly higher %HYPO-He values [11.6 (4.2-27.6)] as compared to groups A [0.3 (0.2-0.3)], C [1.9 (0.6-4.6)], D [0.6 (0.4-0.8)] (all P < 0.0001), while there was no statistically significant difference of %HYPO-He values between heterozygous Hb O-Arab and groups A and C (P = 0.965 & P = 0.134, respectively) based on Tukey post hoc test. Heterozygous β-thalassaemia presents statistically significantly higher %MicroR values [41.5 (22.9-58.7)] as compared to groups A [1.5 (1.1-2.0)], C [10.8 (7.9-20.5)] and D [6.1 (4.2-9.0)] (all P < 0.0001). Both parameters %HYPO-He & %MicroR are correlated positively in a statistically significant degree with Hb A2 levels in heterozygous β-thalassaemia (r = 0.567, P < 0.0001, y = 0.050x + 5.317 & r = 0.680, P < 0.0001, y = 0.042x + 4.371, respectively).

Conclusion
β-thalassaemia heterozygotes present higher values of %HYPO-He & %MicroR compared to α-thalassaemia and Hb O-Arab heterozygotes. %HYPO-He & %MicroR are possibly useful parameters for evaluating the relative severity of different genotypes in heterozygous β-thalassaemia because of the statistically significant positive correlation with Hb A2 levels.

Session topic: 26. Thalassemias

Keyword(s): Red blood cell, Hemoglobinopathy, Anemia

Premium Sponsors

What's new at EHA

Browse the open-source material from EHA past Congresses and meetings.

References

EHA Terms & Conditions
2025 ©
European Hematology Association
USER TERMS AND CONDITIONS / PRIVACY POLICY
(Amended according to GDPR)

By clicking “Accept Terms & all Cookies” or by continuing to browse, you agree to the storing of third-party cookies on your device to enhance your user experience and agree to the user terms and conditions of this learning management system (LMS).

Cookie Settings
Accept Terms & all Cookies