Contributions
Abstract: PB2196
Type: Publication Only
Background
We have tested three different dedicated haemoglobin separation devices for their capacity of performing the diagnostics of hemoglobinopathies. These involve the Variant II TM HPLC (BioRad), the Capillarys2 capillary electrophoresis (Sebia) and the most recently introduced HPLC, Premier Hb9210TM High Resolution HPLC of Trinity Biotech (Menarini).
Aims
As the latter device is new to the market a multisite precision study was performed testing the reproducibility of the system across three test sites (Leiden, Genoa and London) using the same set of samples for several following days. The results between these three sites were compared and evaluated. Moreover we have tested the capacity to detect the most common structural haemoglobin variants, such as HbS, HbC, HbD, HbE and less common Hb variants important to be diagnosed in multi-ethnic populations found in the U.K., The Netherlands and Northern Italy as well as elevated HbA2, as indicator for beta-thalassemia carriers.
Methods
tHb variant separation using he Variant II TM HPLC (BioRad), the Capillarys2 capillary electrophoresis (Sebia) and the most recently introduced HPLC, Premier Hb9210TM High Resolution HPLC of Trinity Biotech (Menarini). Molecular analysis to verify the hemoglobin variants found.
Results
We present the data of the comparison studies using the replicates of the three different sites for the Premier Hb9210TM and of 100 normal samples and 217 patient samples for a variety of beta-thalassemia trait and haemoglobin (Hb) variants, including the molecular data of the beta-thalassemia mutations and Hb variants.
Conclusion
All three apparatuses identified the common Hb variants and beta-thalassemia trait in carriers, homo-, hetero- and compound heterozygotes with the expected sensitivity and specificity. The Premier Hb9210TM High Resolution HPLC of Trinity Biotech shows comparible separation and quantitation on the three different sites using the same samples and is suitable for the analysis of samples suspected of having hemoglobinopathy and the diagnosis of beta-thalassemia trait or Hb variants.
Session topic: 26. Thalassemias
Keyword(s): Thalassemia, Hemoglobinopathy, Hemoglobin variants, Diagnosis
Abstract: PB2196
Type: Publication Only
Background
We have tested three different dedicated haemoglobin separation devices for their capacity of performing the diagnostics of hemoglobinopathies. These involve the Variant II TM HPLC (BioRad), the Capillarys2 capillary electrophoresis (Sebia) and the most recently introduced HPLC, Premier Hb9210TM High Resolution HPLC of Trinity Biotech (Menarini).
Aims
As the latter device is new to the market a multisite precision study was performed testing the reproducibility of the system across three test sites (Leiden, Genoa and London) using the same set of samples for several following days. The results between these three sites were compared and evaluated. Moreover we have tested the capacity to detect the most common structural haemoglobin variants, such as HbS, HbC, HbD, HbE and less common Hb variants important to be diagnosed in multi-ethnic populations found in the U.K., The Netherlands and Northern Italy as well as elevated HbA2, as indicator for beta-thalassemia carriers.
Methods
tHb variant separation using he Variant II TM HPLC (BioRad), the Capillarys2 capillary electrophoresis (Sebia) and the most recently introduced HPLC, Premier Hb9210TM High Resolution HPLC of Trinity Biotech (Menarini). Molecular analysis to verify the hemoglobin variants found.
Results
We present the data of the comparison studies using the replicates of the three different sites for the Premier Hb9210TM and of 100 normal samples and 217 patient samples for a variety of beta-thalassemia trait and haemoglobin (Hb) variants, including the molecular data of the beta-thalassemia mutations and Hb variants.
Conclusion
All three apparatuses identified the common Hb variants and beta-thalassemia trait in carriers, homo-, hetero- and compound heterozygotes with the expected sensitivity and specificity. The Premier Hb9210TM High Resolution HPLC of Trinity Biotech shows comparible separation and quantitation on the three different sites using the same samples and is suitable for the analysis of samples suspected of having hemoglobinopathy and the diagnosis of beta-thalassemia trait or Hb variants.
Session topic: 26. Thalassemias
Keyword(s): Thalassemia, Hemoglobinopathy, Hemoglobin variants, Diagnosis