Contributions
Abstract: PB2189
Type: Publication Only
Background
Several studies reported a high incidence of thromboembolic events in β - thalassemia, more frequent in thalassemia intermedia than in regularly transfused thalassemia major. In these patients a chronic hypercoagulable state is evident and the red blood cells exhibit impaired flow properties that facilitate micro-circulatory disorders.
Aims
Since many abnormalities described in thalassemia may determine rheological alterations, in this study we have investigated the viscoelastic profiles of red blood cells from patients with β-thalassemia. The hemorheological profiles of blood samples obtained from healthy subjects and thalassemic patients were studied by chemometric tools in order to develop a model of prediction of circulatory disorders according to the viscoelastic behaviour.
Methods
Blood samples from 45 β-thalassemia patients and 48 healthy individuals, after informed consent, were analyzed. Hemorheologic profiles were investigated at 37 °C at native and normalized hematocrit. The evaluation of RBCs viscoelastic properties was performed by determining storage modulus G’, loss modulus G' and complex modulus G* in oscillation mode as a function of angular frequency ω in the range 0.1-10 Hz. Multivariate statistical analysis was performed on the resulting G’, G’’ and G* curves and Principal Components Analysis was used as display method.
Results
The hemorheological profiles of patients affected by ß-thalassemia and healthy subjects showed significant differences and the chemometric analysis demonstrated a clearly identification of anemic status according to viscoelastic profile. Increased G’, G’’ and G* modula were observed in thalassemic patients demonstrating a reduction in deformability and impaired flow properties.
Conclusion
In this study a characterization of haemorheological alterations in thalassemia patients has been performed by a chemometric approach. The achieved results permit to consider the viscoelastic properties as promising predictive new indices of microvascular damage in β-thalassemia and to explain the increased incidence of vascular complications in these disorders.
Session topic: 26. Thalassemias
Keyword(s): Thalassemia, Red blood cell
Abstract: PB2189
Type: Publication Only
Background
Several studies reported a high incidence of thromboembolic events in β - thalassemia, more frequent in thalassemia intermedia than in regularly transfused thalassemia major. In these patients a chronic hypercoagulable state is evident and the red blood cells exhibit impaired flow properties that facilitate micro-circulatory disorders.
Aims
Since many abnormalities described in thalassemia may determine rheological alterations, in this study we have investigated the viscoelastic profiles of red blood cells from patients with β-thalassemia. The hemorheological profiles of blood samples obtained from healthy subjects and thalassemic patients were studied by chemometric tools in order to develop a model of prediction of circulatory disorders according to the viscoelastic behaviour.
Methods
Blood samples from 45 β-thalassemia patients and 48 healthy individuals, after informed consent, were analyzed. Hemorheologic profiles were investigated at 37 °C at native and normalized hematocrit. The evaluation of RBCs viscoelastic properties was performed by determining storage modulus G’, loss modulus G' and complex modulus G* in oscillation mode as a function of angular frequency ω in the range 0.1-10 Hz. Multivariate statistical analysis was performed on the resulting G’, G’’ and G* curves and Principal Components Analysis was used as display method.
Results
The hemorheological profiles of patients affected by ß-thalassemia and healthy subjects showed significant differences and the chemometric analysis demonstrated a clearly identification of anemic status according to viscoelastic profile. Increased G’, G’’ and G* modula were observed in thalassemic patients demonstrating a reduction in deformability and impaired flow properties.
Conclusion
In this study a characterization of haemorheological alterations in thalassemia patients has been performed by a chemometric approach. The achieved results permit to consider the viscoelastic properties as promising predictive new indices of microvascular damage in β-thalassemia and to explain the increased incidence of vascular complications in these disorders.
Session topic: 26. Thalassemias
Keyword(s): Thalassemia, Red blood cell