Contributions
Abstract: PB2167
Type: Publication Only
Background
The balance between immunostimulation and immunoregulation in T cell immunity is achieved by a Th1/Th2/Th3/Tr1 and CD4+CD25+ regulatory T (Treg) cell paradigm.
Aims
We investigated the production of type1 (IFN-gamma, NK1), type2 (IL-13, NK2), type3 (TGF-beta, NK3) and regulatory cytokines (IL10, NKr) from human peripheral blood to discuss the cytokine paradigm of NK cells in human allogeneic hematopoietic stem cells transplantation (allo-HSCT).
Methods
Forty patients undergoing haploidentical (n = 27) and HLA-identical sibling (n = 13) allo-HSCT between August 2009 and December 2009 were enrolled in this analysis after being originally selected using a protocol exploring the association of reconstituted donor derived NK1/NK2/NK3/NKr cells to GVHD and CMV reactivation.
Results
Expansion of NK2 and NK3 were found post allo-HSCT compared to healthy donor. The levels of NKr reconstituted to donor’s level since day 15 post allo-HSCT, and the levels of NK1 in recipients post transplantation were consistently lower compared to donors’ levels until day 60 post allo-HSCT. Multivariate analysis showed that the higher levels of NK1 by day 15 were associated with lower overall acute GVHD (HR 0.157, 0.039-0.642, P=0.010) as well as II-IV acute GVHD (HR 0.260, 95%CI, 0.064-1.053, P=0.059). Meanwhile, the higher levels of NK1 by day 15 correlated with lower CMV reactivation (HR 0.040, 0.005-0.348, P=0.003).
Conclusion
These results indicate that rapid reconstitution of NK cells; especially NK1 cells would be help to prevent the development of graft-versus-host disease as well as CMV reactivation after allogeneic transplantation.
Session topic: 22. Stem cell transplantation - Clinical
Keyword(s): Natural killer, Interferon-gamma, Graft-versus-host disease (GVHD), CMV infection
Abstract: PB2167
Type: Publication Only
Background
The balance between immunostimulation and immunoregulation in T cell immunity is achieved by a Th1/Th2/Th3/Tr1 and CD4+CD25+ regulatory T (Treg) cell paradigm.
Aims
We investigated the production of type1 (IFN-gamma, NK1), type2 (IL-13, NK2), type3 (TGF-beta, NK3) and regulatory cytokines (IL10, NKr) from human peripheral blood to discuss the cytokine paradigm of NK cells in human allogeneic hematopoietic stem cells transplantation (allo-HSCT).
Methods
Forty patients undergoing haploidentical (n = 27) and HLA-identical sibling (n = 13) allo-HSCT between August 2009 and December 2009 were enrolled in this analysis after being originally selected using a protocol exploring the association of reconstituted donor derived NK1/NK2/NK3/NKr cells to GVHD and CMV reactivation.
Results
Expansion of NK2 and NK3 were found post allo-HSCT compared to healthy donor. The levels of NKr reconstituted to donor’s level since day 15 post allo-HSCT, and the levels of NK1 in recipients post transplantation were consistently lower compared to donors’ levels until day 60 post allo-HSCT. Multivariate analysis showed that the higher levels of NK1 by day 15 were associated with lower overall acute GVHD (HR 0.157, 0.039-0.642, P=0.010) as well as II-IV acute GVHD (HR 0.260, 95%CI, 0.064-1.053, P=0.059). Meanwhile, the higher levels of NK1 by day 15 correlated with lower CMV reactivation (HR 0.040, 0.005-0.348, P=0.003).
Conclusion
These results indicate that rapid reconstitution of NK cells; especially NK1 cells would be help to prevent the development of graft-versus-host disease as well as CMV reactivation after allogeneic transplantation.
Session topic: 22. Stem cell transplantation - Clinical
Keyword(s): Natural killer, Interferon-gamma, Graft-versus-host disease (GVHD), CMV infection