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IN VITRO AND IN VIVO EVIDENCES OF SICKLING REVERSAL INDUCED BY REHYDRATION WITH HIGH K+-ISOTONIC SOLUTION
Author(s):
Olutayo Ifedayo Ajayi
,
Olutayo Ifedayo Ajayi
Affiliations:
PHYSIOLOGY,UNIVERSITY OF BENIN,Benin city,Nigeria
Oludolapo Uchegbu
,
Oludolapo Uchegbu
Affiliations:
Medical Lab Science,UNIVERSITY OF BENIN,Benin city,Nigeria
Fortune Ozimede
Fortune Ozimede
Affiliations:
PHYSIOLOGY,UNIVERSITY OF BENIN,Benin city,Nigeria
(Abstract release date: 05/18/17) EHA Library. Ajayi O. 05/18/17; 182861; PB2148
Dr. Olutayo Ifedayo Ajayi
Dr. Olutayo Ifedayo Ajayi
Contributions
PDF Abstract
Abstract

Abstract: PB2148

Type: Publication Only

Background
Erythrocyte sickling and adhesion are favoured by cellular dehydration, which increases the rate of hemoglobin polymerization and cell sickling. Potassium chloride co-transport and calcium-activated potassium channel (Gardos channel) mediate erythrocyte dehydration in sickle cell disease and β-thalassemia. We investigated the in-vitro and in-vivo effects of various concentration of K+ ions in physiological solutions (PSS) as well as in cocos nucifera water (CNw) which is known for its natural high potassium content and isotonicity.

Aims
This study was aimed at ascertain the efficacy of high potassium isotonic solutions in rehydrating sickle cell and possibly reversing the sickling phenomenon at in vivo and in vitro situations

Methods

: Erythrocytes from twenty sickle cell anaemia (SCA) as well as 46 healthy subjects were studied. One part was treated with sodium metabisulphite (Na2S5O7) solution to induce maximum sickling as controls while the other was subjected to different high concentrations of K+ in PSS as well as Cocos nucifera water (40mM, 80mM and CNw - 65mmol/L) respectively. The procedure was repeated for the normal HB AA subjects. Also, both groups of subjects were given 10ml/kg body weight of coconut water to drink as a single dose for the in-vivo experiment. Blood samples were collected longitudinally before and after the oral ingestion, at 1hr and at 24hrs for analysis of red cell indices as well as stained blood films used to ascertain the percentage sickled erythrocytes count before and after the treatment in both cases.

Results

Maximum percentage counts of sickled cells after the addition of Na2S5O7 (45%) were observed which decreased significantly (P< 0.05, respectively) to about 2% with Cocos nucifera and 10% with 80mM K+ PSS. The count in 40mM K+-PSS was not statistically significant. In both Hb AA and SS subjects, MCH and MCV remained relatively stable when compared with the pre-ingestion sample (P>0.05, respectively) while MCHC increased significantly in both groups as early as 1hr and sustained till the 24th hour. MCHC was equally raised in the in-vitro samples (P< 0.05, respectively). The morphology of red cells also indicated a lesser count of sickled red cells after the oral ingestion

Conclusion

Cocos nucifera water and other high potassium ion solutions can activate the rehydration of sickled erythrocytes by probably de-activating the Gardos channel to increase the mean corpuscular haemoglobin concentration(MCHC) and thereby restoring the normal red cell shape. We suggest a probable pharmacological value of the cocos nucifera water as well as other formulated high potassium but isotonic fluids in SCA management.

Session topic: 25. Sickle cell disease

Abstract: PB2148

Type: Publication Only

Background
Erythrocyte sickling and adhesion are favoured by cellular dehydration, which increases the rate of hemoglobin polymerization and cell sickling. Potassium chloride co-transport and calcium-activated potassium channel (Gardos channel) mediate erythrocyte dehydration in sickle cell disease and β-thalassemia. We investigated the in-vitro and in-vivo effects of various concentration of K+ ions in physiological solutions (PSS) as well as in cocos nucifera water (CNw) which is known for its natural high potassium content and isotonicity.

Aims
This study was aimed at ascertain the efficacy of high potassium isotonic solutions in rehydrating sickle cell and possibly reversing the sickling phenomenon at in vivo and in vitro situations

Methods

: Erythrocytes from twenty sickle cell anaemia (SCA) as well as 46 healthy subjects were studied. One part was treated with sodium metabisulphite (Na2S5O7) solution to induce maximum sickling as controls while the other was subjected to different high concentrations of K+ in PSS as well as Cocos nucifera water (40mM, 80mM and CNw - 65mmol/L) respectively. The procedure was repeated for the normal HB AA subjects. Also, both groups of subjects were given 10ml/kg body weight of coconut water to drink as a single dose for the in-vivo experiment. Blood samples were collected longitudinally before and after the oral ingestion, at 1hr and at 24hrs for analysis of red cell indices as well as stained blood films used to ascertain the percentage sickled erythrocytes count before and after the treatment in both cases.

Results

Maximum percentage counts of sickled cells after the addition of Na2S5O7 (45%) were observed which decreased significantly (P< 0.05, respectively) to about 2% with Cocos nucifera and 10% with 80mM K+ PSS. The count in 40mM K+-PSS was not statistically significant. In both Hb AA and SS subjects, MCH and MCV remained relatively stable when compared with the pre-ingestion sample (P>0.05, respectively) while MCHC increased significantly in both groups as early as 1hr and sustained till the 24th hour. MCHC was equally raised in the in-vitro samples (P< 0.05, respectively). The morphology of red cells also indicated a lesser count of sickled red cells after the oral ingestion

Conclusion

Cocos nucifera water and other high potassium ion solutions can activate the rehydration of sickled erythrocytes by probably de-activating the Gardos channel to increase the mean corpuscular haemoglobin concentration(MCHC) and thereby restoring the normal red cell shape. We suggest a probable pharmacological value of the cocos nucifera water as well as other formulated high potassium but isotonic fluids in SCA management.

Session topic: 25. Sickle cell disease

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