Contributions
Abstract: PB2123
Type: Publication Only
Background
Aims
In theory, formation of platelet clusters in the presence of EDTA requires functional adhesion molecules, so platelet adhesion and aggregation tests are expected to be in normal range. We aimed to investigate the capacity of simple platelet function analizers for making the distinction between pseudo TCP and real thrombocytopenia.
Methods
Platelet functions were measured as collagen-ADP and collagen-epinephrine closure times (ColADP and ColEPI) by Platelet Function Analyzer (PFA-200™) for all patients who are referred to our clinic as thrombocytopenia (TCP, plt <150 x103/µL) and value of this new method for determining peseudoTCP is compared with PBS which is accepted as the gold standard by using Receiver Operating Characteristic (ROC) curve analysis. PFA-200 system closure time is expected to be longer in true thrombocytopenia and normal in pseudoTCP, but there is no study investigated this system for this purpose. Descriptive analyses were presented using means ±standard deviations for normally distributed variables or median and interquartile range (IQR) for nonparametric continuous variables. An overall p-value of less than 0.05 was considered to show a statistically significant result. This study is supported by Duzce University with project number of 2015.04.03.370 and these are preliminary results.
Results
We included 59 patients who were referred to our clinic with thrombocytopenia (TCP, Plt<150 x103/µL) and 11 healthy controls (Plt>150 x103/µL). Median age was 54 (IQR:37-68) for thrombocytopenic subjects and 37 (%63) of them were female. Median Plt count was 61 x103/µL (IQR:30-90) in TCP group but WBC and Hb were not different from control subjects. Subjects referred with TCP were grouped with PBS as pseudo-TCP and real-TCP. There was no difference in terms of Plt, MPV, PCT, WBC or Hb between these groups but age was younger (median age 46 vs 62, p<0.05) and PDW was higher in pseudoTCP group (med 17.6 vs 16.8, p<0.01). ColEPI and ColADP measures were significantly lower (med 125 vs 287 for ColEPI, med 84 vs 224 for ColADP, p<0.001 for both) at pseudoTCP group.
Conclusion
We concluded that, running PFA tests for everybody with thrombocytopenic counts, could be used for differentiate pseudoTCP and realTCP in centers which does not have conditions for proper BS. Especially long closure times excludes pseudoTCP with a high specificity and could make clinicians quick decisions for further investigations.
Session topic: 32. Platelets disorders
Keyword(s): Thrombocytopenia, Platelet function, Anticoagulants, Aggregation
Abstract: PB2123
Type: Publication Only
Background
Aims
In theory, formation of platelet clusters in the presence of EDTA requires functional adhesion molecules, so platelet adhesion and aggregation tests are expected to be in normal range. We aimed to investigate the capacity of simple platelet function analizers for making the distinction between pseudo TCP and real thrombocytopenia.
Methods
Platelet functions were measured as collagen-ADP and collagen-epinephrine closure times (ColADP and ColEPI) by Platelet Function Analyzer (PFA-200™) for all patients who are referred to our clinic as thrombocytopenia (TCP, plt <150 x103/µL) and value of this new method for determining peseudoTCP is compared with PBS which is accepted as the gold standard by using Receiver Operating Characteristic (ROC) curve analysis. PFA-200 system closure time is expected to be longer in true thrombocytopenia and normal in pseudoTCP, but there is no study investigated this system for this purpose. Descriptive analyses were presented using means ±standard deviations for normally distributed variables or median and interquartile range (IQR) for nonparametric continuous variables. An overall p-value of less than 0.05 was considered to show a statistically significant result. This study is supported by Duzce University with project number of 2015.04.03.370 and these are preliminary results.
Results
We included 59 patients who were referred to our clinic with thrombocytopenia (TCP, Plt<150 x103/µL) and 11 healthy controls (Plt>150 x103/µL). Median age was 54 (IQR:37-68) for thrombocytopenic subjects and 37 (%63) of them were female. Median Plt count was 61 x103/µL (IQR:30-90) in TCP group but WBC and Hb were not different from control subjects. Subjects referred with TCP were grouped with PBS as pseudo-TCP and real-TCP. There was no difference in terms of Plt, MPV, PCT, WBC or Hb between these groups but age was younger (median age 46 vs 62, p<0.05) and PDW was higher in pseudoTCP group (med 17.6 vs 16.8, p<0.01). ColEPI and ColADP measures were significantly lower (med 125 vs 287 for ColEPI, med 84 vs 224 for ColADP, p<0.001 for both) at pseudoTCP group.
Conclusion
We concluded that, running PFA tests for everybody with thrombocytopenic counts, could be used for differentiate pseudoTCP and realTCP in centers which does not have conditions for proper BS. Especially long closure times excludes pseudoTCP with a high specificity and could make clinicians quick decisions for further investigations.
Session topic: 32. Platelets disorders
Keyword(s): Thrombocytopenia, Platelet function, Anticoagulants, Aggregation