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IMMUNE THROMBOCYTOPENIA. EGYPTIAN EXPERIENCE WITH STUDY OF IL-17,TGFB, IL-35 AND IL-12 CYTOKINES IN CHRONIC AND PERSISTENT IMMUNE THROMBOCYTOPENIA PATIENTS.
Author(s):
Noha El Husseiny
Noha El Husseiny
Affiliations:
kasr al aini hospital,cairo,Egypt
(Abstract release date: 05/18/17) EHA Library. El Husseiny N. 05/18/17; 182828; PB2114
Noha El Husseiny
Noha El Husseiny
Contributions
PDF Abstract
Abstract

Abstract: PB2114

Type: Publication Only

Background
The role of T cells in the pathophysiology of immune thrombocytopenia (ITP) is heterogeneous and complex.It has been studied in active and reactive ITP but not to same extend in chronic and persistent type.

Aims
In this study we review the demographic features of 150 immune thrombocytopenic Egyptian patients and for cases who were chronic and persistent with negative both autoimmune screen and virology for hepatitis B and C

Methods
we measured IL-12, IL-35, IL-17 and TGF-β by ELISA to assess role of subtypes of T cells in the pathophysiology of ITP

Results

Our results revealed Chronic and persistent cases who fulfilled the criteria for cytokine assay were 45 cases with a mean (± SD) age of 31.60 ± 8.78 years. Thirty two patients were presented by skin manifestations (71.1%).Eight patients presented with mucous bleeding (17.8%) and five patients presented by combined skin and mucous membrane bleeding (11.1%). Comparison between the cases studied and control groups revealed statistically significant lower platelet count in cases rather than the control. While the four measured cytokines were statistically significant higher in cases rather than the control. Correlation between platelet count and the level of cytokines was statistically insignificant. All cases were under treatment by low dose corticosteroid in addition to another immunesuppression medication. No correlation between measured cytokines and platelet count.

Conclusion
Conclusion: the higher expression of IL-12 and IL-35 is due to persistently higher TH1 activity which explain continuity of the disease.while the higher expression of Treg cytokines (IL-17 and TGF-B) may be explained by effect of immune suppression use or up regulation of their receptors on Treg cells which have resistance to their activity. In chronic ITP, the level of Tcell cytokines can’t predict the course of disease.

Session topic: 32. Platelets disorders

Keyword(s): Immune thrombocytopenia (ITP)

Abstract: PB2114

Type: Publication Only

Background
The role of T cells in the pathophysiology of immune thrombocytopenia (ITP) is heterogeneous and complex.It has been studied in active and reactive ITP but not to same extend in chronic and persistent type.

Aims
In this study we review the demographic features of 150 immune thrombocytopenic Egyptian patients and for cases who were chronic and persistent with negative both autoimmune screen and virology for hepatitis B and C

Methods
we measured IL-12, IL-35, IL-17 and TGF-β by ELISA to assess role of subtypes of T cells in the pathophysiology of ITP

Results

Our results revealed Chronic and persistent cases who fulfilled the criteria for cytokine assay were 45 cases with a mean (± SD) age of 31.60 ± 8.78 years. Thirty two patients were presented by skin manifestations (71.1%).Eight patients presented with mucous bleeding (17.8%) and five patients presented by combined skin and mucous membrane bleeding (11.1%). Comparison between the cases studied and control groups revealed statistically significant lower platelet count in cases rather than the control. While the four measured cytokines were statistically significant higher in cases rather than the control. Correlation between platelet count and the level of cytokines was statistically insignificant. All cases were under treatment by low dose corticosteroid in addition to another immunesuppression medication. No correlation between measured cytokines and platelet count.

Conclusion
Conclusion: the higher expression of IL-12 and IL-35 is due to persistently higher TH1 activity which explain continuity of the disease.while the higher expression of Treg cytokines (IL-17 and TGF-B) may be explained by effect of immune suppression use or up regulation of their receptors on Treg cells which have resistance to their activity. In chronic ITP, the level of Tcell cytokines can’t predict the course of disease.

Session topic: 32. Platelets disorders

Keyword(s): Immune thrombocytopenia (ITP)

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