Contributions
Abstract: PB2097
Type: Publication Only
Background
Although the etiology of immune thrombocytopenic purpura (ITP) remains unclear, both genetic and environmental factors may contribute to the development of disease. Tumor necrosis factor alpha & beta (TNF-α and TNF-β) are important pro-inflammatory cytokines that play a role in regulation of cell differentiation, proliferation and death, as well as in inflammation, innate and adaptive immune responses, and have been implicated in a wide variety of human diseases. We hypothesized that inflammatory cytokine genes polymorphisms (TNF-α and TNF-β) in ITP pediatric patients may play a fundamental role in pathogenesis of the chronic course of the disease and this might be the base for future specific immunomodulatory therapies for chronic ITP (cITP) in children.
Aims
Methods
The current study included 80 Egyptian cITP patients at Pediatric Hematology Unit, Cairo University (mean age 7.08± 3.64 years) and 100 matched unrelated healthy controls. Genotyping was performed using polymerase chain reaction restriction fragment length polymorphism technique (PCR-RFLP).
Results
Conclusion
We hereby report a strong association between combined polymorphisms of both TNF-α & TNF-β genes and susceptibility to chronicity of ITP in Egyptian children. Further studies for gene polymorphisms which could affect the pathogenesis of ITP and facilitate the development of new therapeutic modalities are recommended.
Session topic: 32. Platelets disorders
Keyword(s): Tumor necrosis factor (TNF), Polymorphism, Cytokine, Chronic ITP
Abstract: PB2097
Type: Publication Only
Background
Although the etiology of immune thrombocytopenic purpura (ITP) remains unclear, both genetic and environmental factors may contribute to the development of disease. Tumor necrosis factor alpha & beta (TNF-α and TNF-β) are important pro-inflammatory cytokines that play a role in regulation of cell differentiation, proliferation and death, as well as in inflammation, innate and adaptive immune responses, and have been implicated in a wide variety of human diseases. We hypothesized that inflammatory cytokine genes polymorphisms (TNF-α and TNF-β) in ITP pediatric patients may play a fundamental role in pathogenesis of the chronic course of the disease and this might be the base for future specific immunomodulatory therapies for chronic ITP (cITP) in children.
Aims
Methods
The current study included 80 Egyptian cITP patients at Pediatric Hematology Unit, Cairo University (mean age 7.08± 3.64 years) and 100 matched unrelated healthy controls. Genotyping was performed using polymerase chain reaction restriction fragment length polymorphism technique (PCR-RFLP).
Results
Conclusion
We hereby report a strong association between combined polymorphisms of both TNF-α & TNF-β genes and susceptibility to chronicity of ITP in Egyptian children. Further studies for gene polymorphisms which could affect the pathogenesis of ITP and facilitate the development of new therapeutic modalities are recommended.
Session topic: 32. Platelets disorders
Keyword(s): Tumor necrosis factor (TNF), Polymorphism, Cytokine, Chronic ITP