Contributions
Abstract: PB2080
Type: Publication Only
Background
Castleman`s disease (CD) is а rare non-clonal lymphoproliferative disorder. Most of the cases are characterized by increased vascularity in the affected tissue. The disease falls into two major histological variants: plasma cell type and hyaline vascular type. However, the correlation between microvessel density and the subtype of the disease has not been established yet.
Aims
To investigate the association between microvessel density and histological type of CD.
Methods
Twenty-eight lymph nodes from patients diagnosed with CD were used for the study. The age of the patients ranged from 24 to 65 years, 14 were male and 14 were female. Three nodes without evidence of metastasis removed for breast cancer were used as controls. The diagnosis of hyaline vascular CD was based on overall preserved immunoarchitecture with typical angio-follicular hyperplasia, circular arrangement of mantle cells around hyalinized germinal centers (“onion skin” pattern). The plasma cell type of CD was confirmed by presence of perifollicular sheets of CD138+ plasma cells. Vessels were labeled with СD34 immunostain. Slides were scanned by the whole slide digital Panoramic scanner. Percentage of blood vessel area (vessel density index) was calculated using Panoramic Viewer software, statistical analysis was conducted with Student’s t-test.
Results
The plasma cell variant of CD was diagnosed in 8 patients, the hyaline vascular variant – in 20 patients. In control group vessels occupied 10±1,0% of the area. In patients with plasma cell variant percentage of blood vessel area was increased to 15.1±1.4% (p<0,05). Patients with hyaline vascular CD were divided into 2 groups depending on the vessel density index. In 15 patients (75%) percentage of vessel area was 6.8±2.3%, which was somewhat lower than in patients with plasma cell variant (not statistically significant). In 5 patients (25%) with hyaline vascular CD, the percentage of vessel area was higher - 12,3±1,5% (p<0.05) and did not differ from levels in patients with plasma cell variant.
Conclusion
Session topic: 18. Non-Hodgkin & Hodgkin lymphoma - Biology
Keyword(s): Castleman's disease, Angiogenesis
Abstract: PB2080
Type: Publication Only
Background
Castleman`s disease (CD) is а rare non-clonal lymphoproliferative disorder. Most of the cases are characterized by increased vascularity in the affected tissue. The disease falls into two major histological variants: plasma cell type and hyaline vascular type. However, the correlation between microvessel density and the subtype of the disease has not been established yet.
Aims
To investigate the association between microvessel density and histological type of CD.
Methods
Twenty-eight lymph nodes from patients diagnosed with CD were used for the study. The age of the patients ranged from 24 to 65 years, 14 were male and 14 were female. Three nodes without evidence of metastasis removed for breast cancer were used as controls. The diagnosis of hyaline vascular CD was based on overall preserved immunoarchitecture with typical angio-follicular hyperplasia, circular arrangement of mantle cells around hyalinized germinal centers (“onion skin” pattern). The plasma cell type of CD was confirmed by presence of perifollicular sheets of CD138+ plasma cells. Vessels were labeled with СD34 immunostain. Slides were scanned by the whole slide digital Panoramic scanner. Percentage of blood vessel area (vessel density index) was calculated using Panoramic Viewer software, statistical analysis was conducted with Student’s t-test.
Results
The plasma cell variant of CD was diagnosed in 8 patients, the hyaline vascular variant – in 20 patients. In control group vessels occupied 10±1,0% of the area. In patients with plasma cell variant percentage of blood vessel area was increased to 15.1±1.4% (p<0,05). Patients with hyaline vascular CD were divided into 2 groups depending on the vessel density index. In 15 patients (75%) percentage of vessel area was 6.8±2.3%, which was somewhat lower than in patients with plasma cell variant (not statistically significant). In 5 patients (25%) with hyaline vascular CD, the percentage of vessel area was higher - 12,3±1,5% (p<0.05) and did not differ from levels in patients with plasma cell variant.
Conclusion
Session topic: 18. Non-Hodgkin & Hodgkin lymphoma - Biology
Keyword(s): Castleman's disease, Angiogenesis