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POSSIBLE ROLE OF FLOW CYTOMETRY TO CHARACTERIZE INFILTRATING CD4 CELLS IN THE MICRO ENVIRONMENT OF LYMPHOMA TISSUE SAMPLES
Author(s):
Rosa Di Gaetano
,
Rosa Di Gaetano
Affiliations:
Haematology Castelfranco Veneto,Azienda ULSS 2 Marca Trevigiana,Castelfranco Veneto ( TV ),Italy
Donata Belvini
,
Donata Belvini
Affiliations:
Haematology Castelfranco Veneto,Azienda ULSS 2 Marca Trevigiana,Castelfranco Veneto ( TV ),Italy
Barbara Callegari
,
Barbara Callegari
Affiliations:
Haematology Castelfranco Veneto,Azienda ULSS 2 Marca Trevigiana,Castelfranco Veneto ( TV ),Italy
Maria Angela De Benedetto
,
Maria Angela De Benedetto
Affiliations:
Haematology Castelfranco Veneto,Azienda ULSS 2 Marca Trevigiana,Castelfranco Veneto ( TV ),Italy
Elena Pilotto
,
Elena Pilotto
Affiliations:
Haematology Castelfranco Veneto,Azienda ULSS 2 Marca Trevigiana,Castelfranco Veneto ( TV ),Italy
Roberto Sartori
,
Roberto Sartori
Affiliations:
Haematology Castelfranco Veneto,Azienda ULSS 2 Marca Trevigiana,Castelfranco Veneto ( TV ),Italy
Loretta Vassallo
,
Loretta Vassallo
Affiliations:
Pathology Castelfranco Veneto,Azienda ULSS 2 Marca Trevigiana,Castelfranco Veneto ( TV ),Italy
Antonio Scapinello
,
Antonio Scapinello
Affiliations:
Pathology Castelfranco Veneto,Azienda ULSS 2 Marca Trevigiana,Castelfranco Veneto ( TV ),Italy
Tagariello Giuseppe
Tagariello Giuseppe
Affiliations:
Haematology Castelfranco Veneto,Azienda ULSS 2 Marca Trevigiana,Castelfranco Veneto ( TV ),Italy
(Abstract release date: 05/18/17) EHA Library. Di Gaetano R. 05/18/17; 182790; PB2076
Dr. Rosa Di Gaetano
Dr. Rosa Di Gaetano
Contributions
PDF Abstract
Abstract

Abstract: PB2076

Type: Publication Only

Background

In our previous work ( Di Gaetano et al, Ann Haematol, 2014 ) we analyzed by flow cytometry (FC) the rich infiltrated characterizing the microenvironment of Hodgkin lymphoma (HL), mainly comprised of CD4 T lymphocytes . We confirmed that the majority of these CD4 T expressing the activation markers (CD38) but lose the CD26 and we suggested to identify the subset CD4+CD26-CD38+ to identify the non-neoplastic cellular pattern in HL. A subset connectable to regulatory T (Treg) cells, because the low expression of CD26 ( DPP4) added to the presence of CD39 (NTPDase ) may be responsible for the generation of adenosine, which plays a major role in Treg-mediated immunosuppression

Aims

We wanted to test if this subset may also characterize T infiltrating lymphocytes the lymph nodes of Non-Hodgkin's lymphomas (NHL) and to verify the expressions of the two enzymatic markers (CD26 and CD39) in microenvironments of HL and NHL analyzed by FC

Methods

In 2016 we analyze by FC in lymph nodes of 6 HL and in 32 NHL ( 12 DLBCL, 10 FL, 5 SLL, 3 MZL, 2 MCL ) the CD4 T subset testing the expression of CD26, CD38, CD39.

Results

In CD4 T HL, CD39 is expressed in 44% of the subset and the increased presence (50%) of CD4+CD26-CD38+ cells is confirmed. Compared with HL, the cells of DLBCL are not statistically ( t Student test ) different: CD38 ( 64 vs 55; p = 0,39), CD26-CD38+ ( 50 vs 46; p = 0,66 ), CD39 ( 44 vs 59; p = 0,15 ). While HL and FL cells are significantly different: CD38 ( 64 vs 23; p < 0,05 ), CD26-CD38+ ( 50 vs 18; p < 0,05 ), CD39 ( 44 vs 23; p <0,05 ). The other three types of NHL, few in number, show a tendency to a significant difference compared with DLBCL.

Conclusion
The our data show the phenotipic variations in the microenvironements of different types of lymphoma emphasizing of DLBCL the similarity with HL and the difference with FL and other NHL.They also suggest a link between a activated environment (CD38+) and a high CD39, which, in addition to a low CD26, could enhance the generation of adenosine and, therefore, an increased immune suppressive activity. The profile by FC of CD4 T infiltrating can characterize lymphomas in its environment indicating also signals and biological mechanisms representative of possible therapeutic target

Session topic: 18. Non-Hodgkin & Hodgkin lymphoma - Biology

Keyword(s): T regulatory cells, Microenvironment, lymphoma

Abstract: PB2076

Type: Publication Only

Background

In our previous work ( Di Gaetano et al, Ann Haematol, 2014 ) we analyzed by flow cytometry (FC) the rich infiltrated characterizing the microenvironment of Hodgkin lymphoma (HL), mainly comprised of CD4 T lymphocytes . We confirmed that the majority of these CD4 T expressing the activation markers (CD38) but lose the CD26 and we suggested to identify the subset CD4+CD26-CD38+ to identify the non-neoplastic cellular pattern in HL. A subset connectable to regulatory T (Treg) cells, because the low expression of CD26 ( DPP4) added to the presence of CD39 (NTPDase ) may be responsible for the generation of adenosine, which plays a major role in Treg-mediated immunosuppression

Aims

We wanted to test if this subset may also characterize T infiltrating lymphocytes the lymph nodes of Non-Hodgkin's lymphomas (NHL) and to verify the expressions of the two enzymatic markers (CD26 and CD39) in microenvironments of HL and NHL analyzed by FC

Methods

In 2016 we analyze by FC in lymph nodes of 6 HL and in 32 NHL ( 12 DLBCL, 10 FL, 5 SLL, 3 MZL, 2 MCL ) the CD4 T subset testing the expression of CD26, CD38, CD39.

Results

In CD4 T HL, CD39 is expressed in 44% of the subset and the increased presence (50%) of CD4+CD26-CD38+ cells is confirmed. Compared with HL, the cells of DLBCL are not statistically ( t Student test ) different: CD38 ( 64 vs 55; p = 0,39), CD26-CD38+ ( 50 vs 46; p = 0,66 ), CD39 ( 44 vs 59; p = 0,15 ). While HL and FL cells are significantly different: CD38 ( 64 vs 23; p < 0,05 ), CD26-CD38+ ( 50 vs 18; p < 0,05 ), CD39 ( 44 vs 23; p <0,05 ). The other three types of NHL, few in number, show a tendency to a significant difference compared with DLBCL.

Conclusion
The our data show the phenotipic variations in the microenvironements of different types of lymphoma emphasizing of DLBCL the similarity with HL and the difference with FL and other NHL.They also suggest a link between a activated environment (CD38+) and a high CD39, which, in addition to a low CD26, could enhance the generation of adenosine and, therefore, an increased immune suppressive activity. The profile by FC of CD4 T infiltrating can characterize lymphomas in its environment indicating also signals and biological mechanisms representative of possible therapeutic target

Session topic: 18. Non-Hodgkin & Hodgkin lymphoma - Biology

Keyword(s): T regulatory cells, Microenvironment, lymphoma

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