Contributions
Abstract: PB2074
Type: Publication Only
Background
Long noncoding RNAs (lncRNAs) are constantly transcribed and involved in a variety of biological activities. The contributions of lncRNAs to the development of germinal center (GCB)-like diffuse large B-cell lymphoma (DLBCL) remain largely unknown.
Aims
The aim of this study was to investigate the expression profile of lncRNAs in human GCB DLBCL cell lines (OCI-ly1 and OCI-ly19) and normal B lymphocytes by microarray.
Methods
We used Arraystar Human LncRNA Microarray V3.0 for profiling of lncRNAs in our specimens.Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology) with minor modifications. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to confirm the results of six upregulated and two downregulated lncRNAs. Bioinformatic analysis (gene ontology analysis, pathway analysis and network analysis) was performed to predict the biological functions and potential mechanisms of the differentially expressed lncRNAs in GCB DLBCL.
Results
We demonstrated that 21,539 lncRNAs were expressed in all samples analyzed, of which 1,648 lncRNAs were upregulated and 2,671 lncRNAs were downregulated in GCB DLBCL cell lines (OCI-ly1 and OCI-ly19) (≥2.0-fold, P<0.05). Pathway analysis indicated that 64 pathways corresponded to upregulated transcripts, and 62 pathways corresponded to downregulated transcripts (P<0.05). In addition, a lncRNA-mRNA co-expression network was constructed to identify potential target genes related to the 3 upregulated and 2 downregulated lncRNAs.
Conclusion
Our data suggested that lncRNAs may play an important role in the pathogenesis of GCB DLBCL, and profile of lncRNAs may be used as a potential biomarker in the diagnosis of DLBCL and predicting its clinical outcome.
Session topic: 18. Non-Hodgkin & Hodgkin lymphoma - Biology
Keyword(s): Diffuse large B cell lymphoma
Abstract: PB2074
Type: Publication Only
Background
Long noncoding RNAs (lncRNAs) are constantly transcribed and involved in a variety of biological activities. The contributions of lncRNAs to the development of germinal center (GCB)-like diffuse large B-cell lymphoma (DLBCL) remain largely unknown.
Aims
The aim of this study was to investigate the expression profile of lncRNAs in human GCB DLBCL cell lines (OCI-ly1 and OCI-ly19) and normal B lymphocytes by microarray.
Methods
We used Arraystar Human LncRNA Microarray V3.0 for profiling of lncRNAs in our specimens.Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology) with minor modifications. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to confirm the results of six upregulated and two downregulated lncRNAs. Bioinformatic analysis (gene ontology analysis, pathway analysis and network analysis) was performed to predict the biological functions and potential mechanisms of the differentially expressed lncRNAs in GCB DLBCL.
Results
We demonstrated that 21,539 lncRNAs were expressed in all samples analyzed, of which 1,648 lncRNAs were upregulated and 2,671 lncRNAs were downregulated in GCB DLBCL cell lines (OCI-ly1 and OCI-ly19) (≥2.0-fold, P<0.05). Pathway analysis indicated that 64 pathways corresponded to upregulated transcripts, and 62 pathways corresponded to downregulated transcripts (P<0.05). In addition, a lncRNA-mRNA co-expression network was constructed to identify potential target genes related to the 3 upregulated and 2 downregulated lncRNAs.
Conclusion
Our data suggested that lncRNAs may play an important role in the pathogenesis of GCB DLBCL, and profile of lncRNAs may be used as a potential biomarker in the diagnosis of DLBCL and predicting its clinical outcome.
Session topic: 18. Non-Hodgkin & Hodgkin lymphoma - Biology
Keyword(s): Diffuse large B cell lymphoma