Contributions
Abstract: PB1850
Type: Publication Only
Background
Granulocyte colony-stimulating factor (G-CSF) has been widely used to mobilize peripheral blood stem cells. In addition, it also has been tried to reveal the regenerative potential in various neurodegenerative diseases.
Aims
We investigated the short-term and delayed effects of infused G-CSF for peripheral blood stem cell (PBSC) mobilization on the various cytokine secretions in children with cerebral palsy (CP).
Methods
G-CSF (10μg/kg/dose) was administered subcutaneously for 4 days to the children with CP. In first group, blood levels of G-CSF, interleukin (IL)-6, IL-10, insulin-like growth factor (IGF-1), vascular endothelial growth factor (VEGF), and brain derived neurotrophic factor (BDNF) as well as mobilized total nucleated cell (TNC)/CD34+ cell counts in peripheral blood were compared between just before G-CSF injection (D+0) and 1 day after 4 days of G-CSF injections (D+5). In second group, cytokine levels were compared between D+0 and 1 month after 4 days of G-CSF injection (D+30). Cytokine levels were measured by enzyme-linked immunosorbent assay.
Results
Baseline levels of G-CSF were significantly increased (p=0.000) and IGF-1 decreased (p=0.011) at D+5 after 4 days of G-CSF administration compared to control group. In contrast, other cytokine levels including IL-6, IL-10, VEGF, and BDNF did not show any significant changes between before and after G-CSF administration. CD34+ cell counts (p=0.000) as well as TNC counts (p=0.000) were significantly increased from D+0 to D+5 in children who received G-CSF compared to children received placebo. Regarding delayed effect of G-CSF administration, G-CSF levels were significantly increased from baseline to D+30 (p=0.000), along with the increase IL-10 (p=0.035) and VEGF levels (p=0.011) and the decrease of IGF-1levels (p=0.014).
Conclusion
G-CSF which administered to mobilize PBSCs could induce the delayed effects on the levels of G-CSF itself as well as of other cytokines which could affect on the neuroregenerative potential. Further studies would be warranted to reveal the mechanism and clinical significances of these delayed effect of G-CSF or mobilized PBSCs.
Session topic: 24. Gene therapy, cellular immunotherapy and vaccination
Keyword(s): Peripheral blood stem cell, G-CSF mobilization, Cytokine, Children
Abstract: PB1850
Type: Publication Only
Background
Granulocyte colony-stimulating factor (G-CSF) has been widely used to mobilize peripheral blood stem cells. In addition, it also has been tried to reveal the regenerative potential in various neurodegenerative diseases.
Aims
We investigated the short-term and delayed effects of infused G-CSF for peripheral blood stem cell (PBSC) mobilization on the various cytokine secretions in children with cerebral palsy (CP).
Methods
G-CSF (10μg/kg/dose) was administered subcutaneously for 4 days to the children with CP. In first group, blood levels of G-CSF, interleukin (IL)-6, IL-10, insulin-like growth factor (IGF-1), vascular endothelial growth factor (VEGF), and brain derived neurotrophic factor (BDNF) as well as mobilized total nucleated cell (TNC)/CD34+ cell counts in peripheral blood were compared between just before G-CSF injection (D+0) and 1 day after 4 days of G-CSF injections (D+5). In second group, cytokine levels were compared between D+0 and 1 month after 4 days of G-CSF injection (D+30). Cytokine levels were measured by enzyme-linked immunosorbent assay.
Results
Baseline levels of G-CSF were significantly increased (p=0.000) and IGF-1 decreased (p=0.011) at D+5 after 4 days of G-CSF administration compared to control group. In contrast, other cytokine levels including IL-6, IL-10, VEGF, and BDNF did not show any significant changes between before and after G-CSF administration. CD34+ cell counts (p=0.000) as well as TNC counts (p=0.000) were significantly increased from D+0 to D+5 in children who received G-CSF compared to children received placebo. Regarding delayed effect of G-CSF administration, G-CSF levels were significantly increased from baseline to D+30 (p=0.000), along with the increase IL-10 (p=0.035) and VEGF levels (p=0.011) and the decrease of IGF-1levels (p=0.014).
Conclusion
G-CSF which administered to mobilize PBSCs could induce the delayed effects on the levels of G-CSF itself as well as of other cytokines which could affect on the neuroregenerative potential. Further studies would be warranted to reveal the mechanism and clinical significances of these delayed effect of G-CSF or mobilized PBSCs.
Session topic: 24. Gene therapy, cellular immunotherapy and vaccination
Keyword(s): Peripheral blood stem cell, G-CSF mobilization, Cytokine, Children