Contributions
Abstract: PB1843
Type: Publication Only
Background
The differential diagnosis of hereditary hemolytic anemia is generally carried out by applying different diagnostic protocols depending on the specific congenital erythrocyte defects. Thermogravimetric analysis (TGA) coupled with chemometrics has recently been proposed as a rapid and cost effective diagnostic tool for β-thalassemia screening. This model, consisting of Partial Least Square-Discriminant Analysis (PLS-DA), permitted the discrimination of thalassemic patients and healthy individuals, using thermogravimetric curves of blood samples [1].
Aims
In this study, the capability of thermogravimetry in conjuction with a multivariate statistical analysis was investigated for the screening of hereditary hemolytic anemias due to different erythrocyte defects.
Methods
Whole blood samples collected in K2EDTA were obtained, after informed consent, from patients suffering from congenital hemolytic anemias and were analyzed using the thermobalance TG7 (Perkin Elmer) without any pretreatment and the resulting curves were compared with those of healthy individuals. Two groups of hereditary hemolytic anemias were considered: the hemoglobinopathies (sickle cells anemia and thalassemia) and the erythrocyte membrane defects (hereditary elliptocytosis and hereditary spherocytosis).
Results
The characteristic profile of the blood sample thermal decomposition and the first derivative (DTG) of the TG curve showed that blood samples from anemic patients were clearly distinguished from those of healthy individuals as a result of different amounts of water and corpuscular fraction. The chemometric approach based on Principal Components Analysis (PCA) allowed a quick identification of differences between healthy and anemic patients in order to point out a model of prediction in patients with heterogeneous congenital hematological disorders.
Conclusion
The achieved results allow to consider the coupling TGA/Chemometrics as a promising diagnostic approach to provide a high-throughput and sensitive tool to obtain an early detection of hereditary hemolytic anemias using only a few microliters of blood without any pretreatment and with an hour of analysis time.
Session topic: 27. Enzymopathies, membranopathies and other anemias
Keyword(s): Thalassemia, Hereditary spherocytosis, Hemolytic anemia, Diagnosis
Abstract: PB1843
Type: Publication Only
Background
The differential diagnosis of hereditary hemolytic anemia is generally carried out by applying different diagnostic protocols depending on the specific congenital erythrocyte defects. Thermogravimetric analysis (TGA) coupled with chemometrics has recently been proposed as a rapid and cost effective diagnostic tool for β-thalassemia screening. This model, consisting of Partial Least Square-Discriminant Analysis (PLS-DA), permitted the discrimination of thalassemic patients and healthy individuals, using thermogravimetric curves of blood samples [1].
Aims
In this study, the capability of thermogravimetry in conjuction with a multivariate statistical analysis was investigated for the screening of hereditary hemolytic anemias due to different erythrocyte defects.
Methods
Whole blood samples collected in K2EDTA were obtained, after informed consent, from patients suffering from congenital hemolytic anemias and were analyzed using the thermobalance TG7 (Perkin Elmer) without any pretreatment and the resulting curves were compared with those of healthy individuals. Two groups of hereditary hemolytic anemias were considered: the hemoglobinopathies (sickle cells anemia and thalassemia) and the erythrocyte membrane defects (hereditary elliptocytosis and hereditary spherocytosis).
Results
The characteristic profile of the blood sample thermal decomposition and the first derivative (DTG) of the TG curve showed that blood samples from anemic patients were clearly distinguished from those of healthy individuals as a result of different amounts of water and corpuscular fraction. The chemometric approach based on Principal Components Analysis (PCA) allowed a quick identification of differences between healthy and anemic patients in order to point out a model of prediction in patients with heterogeneous congenital hematological disorders.
Conclusion
The achieved results allow to consider the coupling TGA/Chemometrics as a promising diagnostic approach to provide a high-throughput and sensitive tool to obtain an early detection of hereditary hemolytic anemias using only a few microliters of blood without any pretreatment and with an hour of analysis time.
Session topic: 27. Enzymopathies, membranopathies and other anemias
Keyword(s): Thalassemia, Hereditary spherocytosis, Hemolytic anemia, Diagnosis