Contributions
Abstract: PB1703
Type: Publication Only
Background
Translocation and chromosomal anomalies have prognostic implications in acute myeloid leukemia (AML). Cytogenetic analysis assumes great importance in their diagnosis and treatment stratification which are assessed by karyotyping and/or reverse transcriptase polymerase chain reaction (RT-PCR). Given the dependency of karyotyping on sample quality, more and more centers are now relying on RT-PCR to detect specific translocations. Varying rates of concordance between Karyotyping and RT-PCR have been reported and no consensus has prevailed. Given the resource constraint, it is economically non-viable to perform both for prognosis in real world scenarios. In addition, the cost of the extra tests also adds to the burden of healthcare economy.
Aims
In 132 patients of AML, we aimed at determining the incidence of cytogenetic abnormalities and molecular anomalies detected by Karyotyping and RT-PCR respectively. Concordance rates between conventional cytogenetic tests and RT-PCR were also calculated.
Methods
We conducted a retrospective analysis on the medical records of 132 patients of AML at a tertiary health care facility in India, treated during 2010-2017. Results from commercially available molecular assays for detection of specific translocations by RT-PCR and of adequate samples of karyotype analysis were compared.
Results
Conclusion
Session topic: 4. Acute myeloid leukemia - Clinical
Keyword(s): RT-PCR, Cytogenetic abnormalities, AML
Abstract: PB1703
Type: Publication Only
Background
Translocation and chromosomal anomalies have prognostic implications in acute myeloid leukemia (AML). Cytogenetic analysis assumes great importance in their diagnosis and treatment stratification which are assessed by karyotyping and/or reverse transcriptase polymerase chain reaction (RT-PCR). Given the dependency of karyotyping on sample quality, more and more centers are now relying on RT-PCR to detect specific translocations. Varying rates of concordance between Karyotyping and RT-PCR have been reported and no consensus has prevailed. Given the resource constraint, it is economically non-viable to perform both for prognosis in real world scenarios. In addition, the cost of the extra tests also adds to the burden of healthcare economy.
Aims
In 132 patients of AML, we aimed at determining the incidence of cytogenetic abnormalities and molecular anomalies detected by Karyotyping and RT-PCR respectively. Concordance rates between conventional cytogenetic tests and RT-PCR were also calculated.
Methods
We conducted a retrospective analysis on the medical records of 132 patients of AML at a tertiary health care facility in India, treated during 2010-2017. Results from commercially available molecular assays for detection of specific translocations by RT-PCR and of adequate samples of karyotype analysis were compared.
Results
Conclusion
Session topic: 4. Acute myeloid leukemia - Clinical
Keyword(s): RT-PCR, Cytogenetic abnormalities, AML