Contributions
Abstract: PB1674
Type: Publication Only
Background
Aims
Methods
This retrospective analysis included 85 patients with de novo AML diagnosed from January 2005 to December 2015 submitted to BM biopsy at diagnosis. p53 expression was detected by immunohistochemistry, and staining was evaluated using the H-score (range 0-300). The t-test and Mann-Whitney U test were used to detect differences in the distribution of continuous parametric and nonparametric variables, respectively. Overall survival (OS), disease-free survival (DFS) and event-free survival (EFS) were calculated using the Kaplan-Meier method. The log-rank test was used for comparison of survival curves. The interaction between the examined prognostic variables was tested with univariate and multivariate Cox regression analysis.
Results
Median age was 60 years (17-81). There was a predominance of patients >60 years (54.1%) and males (56.5%). The median H-score for p53 was 11.8 (0.4-161.1), with no significant correlation with age or cytogenetic risk. p53 expression was significantly higher in patients with a complex karyotype (p = 0.0031) and high risk by European Leukemia Net (ELN) criteria (p = 0.047). There was a positive correlation with complex karyotype and prognostic risk by ELN. Excluding early deaths (<30 days from induction), patients younger than 60 years with H-score > 60 showed worse overall survival when compared with patients with H-score <60 (0% vs 14.6%, respectively) (p = 0.048). There was no statistical difference in disease-free survival and event-free survival. In the Cox univariate analysis including all cases, peripheral leukocyte counts at diagnosis (p = 0.014), cytogenetic risk groups (p = 0.07), ELN risk categories (p = 0.023) and H-score (p = 0.025) were significant. In a multivariate model including leukocytes, ELN risk and p53, all variables remained in the model.
Conclusion
Session topic: 4. Acute myeloid leukemia - Clinical
Keyword(s): Immunohistochemistry, Acute Myeloid Leukemia, Survival, P53
Abstract: PB1674
Type: Publication Only
Background
Aims
Methods
This retrospective analysis included 85 patients with de novo AML diagnosed from January 2005 to December 2015 submitted to BM biopsy at diagnosis. p53 expression was detected by immunohistochemistry, and staining was evaluated using the H-score (range 0-300). The t-test and Mann-Whitney U test were used to detect differences in the distribution of continuous parametric and nonparametric variables, respectively. Overall survival (OS), disease-free survival (DFS) and event-free survival (EFS) were calculated using the Kaplan-Meier method. The log-rank test was used for comparison of survival curves. The interaction between the examined prognostic variables was tested with univariate and multivariate Cox regression analysis.
Results
Median age was 60 years (17-81). There was a predominance of patients >60 years (54.1%) and males (56.5%). The median H-score for p53 was 11.8 (0.4-161.1), with no significant correlation with age or cytogenetic risk. p53 expression was significantly higher in patients with a complex karyotype (p = 0.0031) and high risk by European Leukemia Net (ELN) criteria (p = 0.047). There was a positive correlation with complex karyotype and prognostic risk by ELN. Excluding early deaths (<30 days from induction), patients younger than 60 years with H-score > 60 showed worse overall survival when compared with patients with H-score <60 (0% vs 14.6%, respectively) (p = 0.048). There was no statistical difference in disease-free survival and event-free survival. In the Cox univariate analysis including all cases, peripheral leukocyte counts at diagnosis (p = 0.014), cytogenetic risk groups (p = 0.07), ELN risk categories (p = 0.023) and H-score (p = 0.025) were significant. In a multivariate model including leukocytes, ELN risk and p53, all variables remained in the model.
Conclusion
Session topic: 4. Acute myeloid leukemia - Clinical
Keyword(s): Immunohistochemistry, Acute Myeloid Leukemia, Survival, P53