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THE EXPRESSION OF SALL4 AND BMI-1 GENES IN MYELOID LEUKEMIA
Author(s):
Mona El-Nagdy
,
Mona El-Nagdy
Affiliations:
Clinical & Chemical Pathology,Cairo University,Cairo,Egypt
Hala Farawela
,
Hala Farawela
Affiliations:
Clinical & Chemical Pathology,Cairo University,Cairo,Egypt
Hamdy Zawam
,
Hamdy Zawam
Affiliations:
Medical Oncology,Cairo University,Cairo,Egypt
Hanan Al-Wakeel
,
Hanan Al-Wakeel
Affiliations:
Clinical & Chemical Pathology,Cairo University,Cairo,Egypt
Fatma El-Refaey
,
Fatma El-Refaey
Affiliations:
Clinical & Chemical Pathology,National Cancer Institute,Cairo,Egypt
Hala Abdel-Rahman
Hala Abdel-Rahman
Affiliations:
Clinical & Chemical Pathology,Cairo University,Cairo,Egypt
(Abstract release date: 05/18/17) EHA Library. El-Nagdy M. 05/18/17; 182372; PB1658
Mona El-Nagdy
Mona El-Nagdy
Contributions
PDF Abstract
Abstract

Abstract: PB1658

Type: Publication Only

Background
Sal-like protein 4 (SALL4) and B-cell specific moloney murine leukemia virus integration site-1 (BMI-1) genes are stem cell genes that modulate stem cell pluripotency and may play a role in leukemogenesis. Leukemic stem cells (LSCs) have been implicated in being the origin of the leukemic blast, therapy resistance and relapse.

Aims
The current study aimed at characterizing the expression pattern of SALL4 and BMI-1 genes in acute myeloid leukemia (AML) and chronic myeloid leukemia (CML), in patients who have achieved complete remission (CR), and in CML disease progression.

Methods
Real-time polymerase chain reaction was used to assess the gene expression patterns in 106 myeloid leukemia patients; 54 de novo AML (43 at time of diagnosis, 11 in CR), and 52 CML (31 in chronic phase (CP), 11 in deep molecular response (MR4) & 10 in accelerated/blastic phase (AP/BP), and in 21 non malignant bone marrow samples.

Results

SALL4 gene expression was increased in AML patients, AML-CR, & CML-CP (median= 5.180, 4.604 & 14.125 respectively). No significant difference was observed between de novo AML and AML in CR patients. CML-CP patients showed a significantly higher percentage of patients with a high SALL4 expression as compared to both CML- MR4 and CML-AP/BP (p=0.033). BMI-1 gene expression was not found to be increased in any of the patient groups.

Conclusion
Our data describe altered SALL4 gene expression in different phases of myeloid leukemia. The role of BMI-1 gene needs further delineation to determine its significance.

Session topic: 3. Acute myeloid leukemia - Biology

Keyword(s): Hematopoietic Stem Cell, Bmi-1, myeloid leukemia

Abstract: PB1658

Type: Publication Only

Background
Sal-like protein 4 (SALL4) and B-cell specific moloney murine leukemia virus integration site-1 (BMI-1) genes are stem cell genes that modulate stem cell pluripotency and may play a role in leukemogenesis. Leukemic stem cells (LSCs) have been implicated in being the origin of the leukemic blast, therapy resistance and relapse.

Aims
The current study aimed at characterizing the expression pattern of SALL4 and BMI-1 genes in acute myeloid leukemia (AML) and chronic myeloid leukemia (CML), in patients who have achieved complete remission (CR), and in CML disease progression.

Methods
Real-time polymerase chain reaction was used to assess the gene expression patterns in 106 myeloid leukemia patients; 54 de novo AML (43 at time of diagnosis, 11 in CR), and 52 CML (31 in chronic phase (CP), 11 in deep molecular response (MR4) & 10 in accelerated/blastic phase (AP/BP), and in 21 non malignant bone marrow samples.

Results

SALL4 gene expression was increased in AML patients, AML-CR, & CML-CP (median= 5.180, 4.604 & 14.125 respectively). No significant difference was observed between de novo AML and AML in CR patients. CML-CP patients showed a significantly higher percentage of patients with a high SALL4 expression as compared to both CML- MR4 and CML-AP/BP (p=0.033). BMI-1 gene expression was not found to be increased in any of the patient groups.

Conclusion
Our data describe altered SALL4 gene expression in different phases of myeloid leukemia. The role of BMI-1 gene needs further delineation to determine its significance.

Session topic: 3. Acute myeloid leukemia - Biology

Keyword(s): Hematopoietic Stem Cell, Bmi-1, myeloid leukemia

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