Contributions
Abstract: P20
Type: Poster presentation
Presentation during EHA Scientific Conference on Bleeding Disorders:
On Friday, September 16, 2016 from 14:00 - 15:30
Location: Cristal + Coral
Background
Often immunoglobulin class G (IgG) appeared and circulated in the patient's bloodstream after disease including cardiovascular. Elevation of the IgG concentration after ischemic stroke was proved previously. Moreover IgG ability to induce releasing from the platelets granules of the certain proteins, fragments and protein complexes was showed in our last research1. It is well known that interaction between IgG and platelets surface causes modulation of the cellular response2-3.
Aim
Was to investigate the healthy donor's platelets aggregation under the influence of IgG separated from the patients with ischemic stroke in the acute stroke as well as year past acute phase of ischemic stroke.
Methods
Blood plasma samples were taken from 35 healthy donors and 66 patients with atherothrombotic ischemic stroke (AIS) and 56 patients with cardioembolic ischemic stroke (CIS) during the acute phase of disease; 57 patients with AIS and 57 patients with CIS one year past acute phase of stroke. IgG was separated from the blood plasma by affinity chromatography4. All separated fractions of IgG were freeze-dried (LyoQuest, Spain), dissolved in vehicle and brought to concentration 300 mkg/ml. ADP-induced platelet aggregation was performs according the standard protocol during the first 2 hours after healthy donor's blood collection on the photo-optical aggregometer AT-02 (Medtech, PF)5. Control platelets aggregation sample included equal volume of vehicle instead of IgG. Statistical processing of the data was done.
Results
Has been shown impact of IgG was characterized by one-wave irreversible ADP-induced platelet aggregation. The influence of the IgG fraction separated from the healthy donors was equal to the control platelets aggregation. The maximum aggregation was shoved under influence of IgG fraction separated from the patients with AIS. This influence was on the 15 % more intensive in comparison with influence of IgG fraction separated from the healthy donors. One year past disease all tested IgG fractions provoked inhibition of platelets aggregation up to 25 %. The maximum inhibition of ADP-dependent healthy donor's platelets aggregation was provided by fraction separated from the patients with AIS one year past acute phase.
Conclusion
Atherothrombotic and cardioembolic ischemic subtypes of acute stroke accompanied by increased concentrations of immunoglobulin class G. All investigated IgG fractions separated from the stroke patients are able to influence certain parts of the haemostasis system instead of the IgG fraction separated blom the healthy donor's blood plasma. In particular IgG separated from the plasma of patients with AIS and CIS in the acute phase have caused the activation of ADP-induced healthy donor's platelets aggregation. In contrast, IgG separated from the plasma of patients with AIS and CIS one year past acute phase have caused inhibition of healthy donor's platelets aggregation. Results could be an evidence of the potentially different immunoglobulin fractions formation in the bloodstream of the patients with different pathology.
References
1. Katrii T. B. et al, International Journal of Chemical and Biomolecular Science 2015; 278-283.
2. Katrii T.B. et al, Jornal Blood Coagulation & Fibrinolisis 2016; Forthcoming.
3. Martin J. et al, Thrombosis research 1983; 443-460.
4. Vovk T.et al, Selected methods for Physics of life 2010; 59-63.
5. Halenova T. I. et al, Ukr. Biochem. J., 2015; 87. 5.
Abstract: P20
Type: Poster presentation
Presentation during EHA Scientific Conference on Bleeding Disorders:
On Friday, September 16, 2016 from 14:00 - 15:30
Location: Cristal + Coral
Background
Often immunoglobulin class G (IgG) appeared and circulated in the patient's bloodstream after disease including cardiovascular. Elevation of the IgG concentration after ischemic stroke was proved previously. Moreover IgG ability to induce releasing from the platelets granules of the certain proteins, fragments and protein complexes was showed in our last research1. It is well known that interaction between IgG and platelets surface causes modulation of the cellular response2-3.
Aim
Was to investigate the healthy donor's platelets aggregation under the influence of IgG separated from the patients with ischemic stroke in the acute stroke as well as year past acute phase of ischemic stroke.
Methods
Blood plasma samples were taken from 35 healthy donors and 66 patients with atherothrombotic ischemic stroke (AIS) and 56 patients with cardioembolic ischemic stroke (CIS) during the acute phase of disease; 57 patients with AIS and 57 patients with CIS one year past acute phase of stroke. IgG was separated from the blood plasma by affinity chromatography4. All separated fractions of IgG were freeze-dried (LyoQuest, Spain), dissolved in vehicle and brought to concentration 300 mkg/ml. ADP-induced platelet aggregation was performs according the standard protocol during the first 2 hours after healthy donor's blood collection on the photo-optical aggregometer AT-02 (Medtech, PF)5. Control platelets aggregation sample included equal volume of vehicle instead of IgG. Statistical processing of the data was done.
Results
Has been shown impact of IgG was characterized by one-wave irreversible ADP-induced platelet aggregation. The influence of the IgG fraction separated from the healthy donors was equal to the control platelets aggregation. The maximum aggregation was shoved under influence of IgG fraction separated from the patients with AIS. This influence was on the 15 % more intensive in comparison with influence of IgG fraction separated from the healthy donors. One year past disease all tested IgG fractions provoked inhibition of platelets aggregation up to 25 %. The maximum inhibition of ADP-dependent healthy donor's platelets aggregation was provided by fraction separated from the patients with AIS one year past acute phase.
Conclusion
Atherothrombotic and cardioembolic ischemic subtypes of acute stroke accompanied by increased concentrations of immunoglobulin class G. All investigated IgG fractions separated from the stroke patients are able to influence certain parts of the haemostasis system instead of the IgG fraction separated blom the healthy donor's blood plasma. In particular IgG separated from the plasma of patients with AIS and CIS in the acute phase have caused the activation of ADP-induced healthy donor's platelets aggregation. In contrast, IgG separated from the plasma of patients with AIS and CIS one year past acute phase have caused inhibition of healthy donor's platelets aggregation. Results could be an evidence of the potentially different immunoglobulin fractions formation in the bloodstream of the patients with different pathology.
References
1. Katrii T. B. et al, International Journal of Chemical and Biomolecular Science 2015; 278-283.
2. Katrii T.B. et al, Jornal Blood Coagulation & Fibrinolisis 2016; Forthcoming.
3. Martin J. et al, Thrombosis research 1983; 443-460.
4. Vovk T.et al, Selected methods for Physics of life 2010; 59-63.
5. Halenova T. I. et al, Ukr. Biochem. J., 2015; 87. 5.