Contributions
Abstract: P17
Type: Poster presentation
Presentation during EHA Scientific Conference on Bleeding Disorders:
On Friday, September 16, 2016 from 14:00 - 15:30
Location: Cristal + Coral
Background
As known ischemic stroke provoked irreversible changes in the organism and fully recovery has not observed1,2. Some reactivity of the haemostasis system was shown during the acute phase of ischemic stroke as well as post few years3. Here the idea was explored that index of organism endo-intoxication4 such as peptide pool with Mr up to 5 kDa generated in the bloodstream during the acute phase and their presence past one year of stroke could provoke disease repetition.
Aim
To characterize qualitative composition of the acute stroke and post stroke PP fraction.
Methods
Fractions of peptide pool (PP) were separated from 10 ml of the blood plasma of each tested group: healthy donors, patients with atherothrombotic (AIS) and cardioembolic ischemic stroke (CIS) in acute phase of disease and the same patients one year past acute phase by the method of Nikolaichyk V5. Concentration was counted in respect to calibration chart and purity was controlled by 15% PAGE6. Qualitatively and quantitatively characterization of each separated PP fractions was performed by the size exclusion chromatographic on the Sephadex G-15 column7. The speed was 30 ml/hour. The column was calibrated by application of the standard markers solution.
Results
Ischemic stroke accompanied by the formation of the peptide pool with Mr up to 5 kDa. Stroke PP fractions were qualitatively and quantitatively differ comparing to healthy donor's PP faction. It was shown that concentration of acute AIS PP fraction was 3 times more higher and for acute CIS 2,5 times than healthy donor's PP fraction. One year past acute phase this correlation was close or identical to donors. Just one peptide with Mr about 1,32 kDa was presented in the healthy donor's PP fraction. The elution volume of this peptide was equal 1,1±0,1 pc. of the total column volume. The same but higher peak was noticed in the stroke PP fractions. Beside this stroke PP fractions include in average 7 peptides. Certain peptides specific for some stroke PP fractions were noticed. The fact is that peptide with Mr 2,95 kDa was typical just for the CIS PP fraction in acute phase as well as past one year. The buffer volume for elution of this peptide was 0,45 ± 0,05 pc. of the column volume. The peptide with Mr 1,45 kDa was typical just for acute AIS PP fraction. The elution volume of this peptide was 0,9 pc. The total area under the peaks was significantly bigger for the one year past acute AIS as well as CIS PP fractions. This value was equal 2631 and 3417 s.u. respectively. The analogical index for the acute stroke PP fractions was equal in averaged 1648 s.u. Healthy donor's PP fraction was characterized by the 263 s.u. of under peaks area.
Discussions
Despite the reduction in PP concentration over time, the year past acute PP fractions had more diverse qualitative composition comparing with acute phase PP fractions. We assumed that components of peptide pool forms complexes with receptors on the platelet membrane and blocked the normal physiological processes in this way. Also it is possible binding formation between PP fractions and other proteins or molecules in plasma which could lids to prevention of the right binding. Perhaps mechanism triggered by the competitive inhibition reactions.
References
1. Shuaib A, Hussain M. Eur. Neurol. 2008; 59: 41-43
2. Woodward М, Lowe D, Camрbell J. Stroke. 2005; 36: 2143–2147
3. Hirsh Jack. Hemostasis and thrombosis: basic principles and clinical practice. 2006
4. Karjakin E.V, Belov S.V. Biochemistry. 2004; 3-8.
5. Nykolaychyk B. B Moyn VM, Kyrkovskyy VV. Laboratornoe case. 1991; 10: 13-18
6. Cleveland, Don W. Journal of Biological Chemistry. 1977; 252.3: 1102-1106
7. Paula H, Stephan K, Edouard S. Journal of Liquid Chromatography&Related Technologies. 2012; 35: 2923–2950
Abstract: P17
Type: Poster presentation
Presentation during EHA Scientific Conference on Bleeding Disorders:
On Friday, September 16, 2016 from 14:00 - 15:30
Location: Cristal + Coral
Background
As known ischemic stroke provoked irreversible changes in the organism and fully recovery has not observed1,2. Some reactivity of the haemostasis system was shown during the acute phase of ischemic stroke as well as post few years3. Here the idea was explored that index of organism endo-intoxication4 such as peptide pool with Mr up to 5 kDa generated in the bloodstream during the acute phase and their presence past one year of stroke could provoke disease repetition.
Aim
To characterize qualitative composition of the acute stroke and post stroke PP fraction.
Methods
Fractions of peptide pool (PP) were separated from 10 ml of the blood plasma of each tested group: healthy donors, patients with atherothrombotic (AIS) and cardioembolic ischemic stroke (CIS) in acute phase of disease and the same patients one year past acute phase by the method of Nikolaichyk V5. Concentration was counted in respect to calibration chart and purity was controlled by 15% PAGE6. Qualitatively and quantitatively characterization of each separated PP fractions was performed by the size exclusion chromatographic on the Sephadex G-15 column7. The speed was 30 ml/hour. The column was calibrated by application of the standard markers solution.
Results
Ischemic stroke accompanied by the formation of the peptide pool with Mr up to 5 kDa. Stroke PP fractions were qualitatively and quantitatively differ comparing to healthy donor's PP faction. It was shown that concentration of acute AIS PP fraction was 3 times more higher and for acute CIS 2,5 times than healthy donor's PP fraction. One year past acute phase this correlation was close or identical to donors. Just one peptide with Mr about 1,32 kDa was presented in the healthy donor's PP fraction. The elution volume of this peptide was equal 1,1±0,1 pc. of the total column volume. The same but higher peak was noticed in the stroke PP fractions. Beside this stroke PP fractions include in average 7 peptides. Certain peptides specific for some stroke PP fractions were noticed. The fact is that peptide with Mr 2,95 kDa was typical just for the CIS PP fraction in acute phase as well as past one year. The buffer volume for elution of this peptide was 0,45 ± 0,05 pc. of the column volume. The peptide with Mr 1,45 kDa was typical just for acute AIS PP fraction. The elution volume of this peptide was 0,9 pc. The total area under the peaks was significantly bigger for the one year past acute AIS as well as CIS PP fractions. This value was equal 2631 and 3417 s.u. respectively. The analogical index for the acute stroke PP fractions was equal in averaged 1648 s.u. Healthy donor's PP fraction was characterized by the 263 s.u. of under peaks area.
Discussions
Despite the reduction in PP concentration over time, the year past acute PP fractions had more diverse qualitative composition comparing with acute phase PP fractions. We assumed that components of peptide pool forms complexes with receptors on the platelet membrane and blocked the normal physiological processes in this way. Also it is possible binding formation between PP fractions and other proteins or molecules in plasma which could lids to prevention of the right binding. Perhaps mechanism triggered by the competitive inhibition reactions.
References
1. Shuaib A, Hussain M. Eur. Neurol. 2008; 59: 41-43
2. Woodward М, Lowe D, Camрbell J. Stroke. 2005; 36: 2143–2147
3. Hirsh Jack. Hemostasis and thrombosis: basic principles and clinical practice. 2006
4. Karjakin E.V, Belov S.V. Biochemistry. 2004; 3-8.
5. Nykolaychyk B. B Moyn VM, Kyrkovskyy VV. Laboratornoe case. 1991; 10: 13-18
6. Cleveland, Don W. Journal of Biological Chemistry. 1977; 252.3: 1102-1106
7. Paula H, Stephan K, Edouard S. Journal of Liquid Chromatography&Related Technologies. 2012; 35: 2923–2950